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Method for detecting algae cell activity by neutral red dyeing method

A neutral red and algal cell technology, applied in biochemical equipment and methods, microbial determination/inspection, instruments, etc., can solve the problems of high cost, time-consuming and cumbersome fluorescent dyeing method, and achieve the effect of simple dyeing operation.

Inactive Publication Date: 2008-06-18
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the time-consuming and cumbersome method of algae cell culture in the prior art when detecting the activity of algae cells, and the use of fluorescent staining requires high-cost instruments, and the fluorescent staining agent is toxic, and its detection effect is also relatively poor , and the detection result is unstable, thus providing a simple, fast and reliable method for detecting algae cell viability using neutral red staining

Method used

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  • Method for detecting algae cell activity by neutral red dyeing method
  • Method for detecting algae cell activity by neutral red dyeing method
  • Method for detecting algae cell activity by neutral red dyeing method

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Embodiment 1

[0023] For a cell density of 2 x 10 9 Each / l Microcystis aeruginosa solution is inactivated, then take 10ml of the inactivated algae cell solution, centrifuge at 3000r / min, discard the supernatant, wash with distilled water, and centrifuge, discard the supernatant solution; wash the precipitate again with distilled water, centrifuge, discard the supernatant to obtain the algal cell precipitate; add 4ml of neutral red dye solution with a concentration of 0.20mg / ml, so that the algal cell precipitate is soaked in the added dye solution During the staining process for 15 minutes, shake well to obtain a cell suspension; use a dropper to absorb a drop of the cell suspension, put it on a glass slide, cover it with a cover glass, and then use an ordinary biological microscope for detection. Record the number B of dead cells stained red by neutral red and the number A of living cells not stained, count 20 fields of view for each sample, and list them in Table 1, and then calculate the...

Embodiment 2

[0029] For a cell density of 5 x 10 8 Each / l of chlorella solution is inactivated, then take 20ml of inactivated algae cell solution, centrifuge at 3000r / min, discard the supernatant, wash with distilled water, and centrifuge, discard the supernatant ; Wash the precipitate again with distilled water, centrifuge, discard the supernatant to obtain the algal cell precipitate; add 4ml of neutral red dye solution with a concentration of 0.15mg / ml, so that the algal cell precipitate is soaked in the added dye solution , stained for 15 minutes, and shaken to obtain a cell suspension; use a dropper to draw a drop of the cell suspension, put it on a glass slide, cover it with a cover glass, and use an ordinary biological microscope to detect. Record the number B of dead cells stained red by neutral red and the number A of living cells not stained, count 20 fields of view for each sample, and list them in Table 2.

[0030] Table 2. Neutral red staining results of Chlorella

[0031] ...

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Abstract

The invention relates to a method to detect the activity of alga cells by means of neutral red staining, which comprises centrifugation of the solution containing alga to be measured; cleaning the solution with distilled water to get the alga cell precipitate; adding neutral red Ringer solution of 0.15mg / ml to 0.20mg / ml to stain the alga cell precipitate for ten to fifteen minutes to get cell suspension; detecting the stained cell number in the cell suspension under ordinary biomicroscopes; numeration of twenty visual fields for each sample, and calculating the cell death rate by using the formula. In the formula, r represents the cell death rate; A represents the number of unstained cells; B represents the number of cells stained red. The method overwhelms the defect of the prior art, and is a convenient and quick method to detect the activity of the frustules by means of neutral red staining.

Description

technical field [0001] The invention relates to a method for detecting algae cell activity by neutral red staining. Background technique [0002] Due to the discharge of a large amount of nitrogen and phosphorus-containing substances into the water body, the eutrophication of the surface water body is caused, resulting in the massive growth and reproduction of algae in the water body, and algal blooms occur in severe cases. The massive growth of algae not only causes the deterioration of the ecological environment, but also has many impacts on people's life and production, such as increasing the difficulty and cost of drinking water treatment, limiting the use of water bodies, etc. Therefore, in order to ensure the safety of water use and achieve the purpose of water use, it is necessary to control the growth of algae and inactivate algae. During the processing of algae, it is often necessary to detect changes in the activity of algae cells. In order to detect the activity...

Claims

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Application Information

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IPC IPC(8): G01N15/00C12Q1/02
Inventor 梁文艳王珂王金丽
Owner BEIJING FORESTRY UNIVERSITY
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