Process for fabricating engineering esophagus imitating biochemistry tissue

A tissue engineering and biomimetic technology, applied in the field of organ preparation and biomimetic tissue, to achieve the effect of maintaining shape and function, promoting growth and exerting biological functions

Inactive Publication Date: 2008-06-25
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This patent only proposes a general method for preparing scaffolds suitable for various organs, and does not make the scaffolds have the unique biological functions of esophageal tissue according to the actual microstructure of the human esophagus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0019] 1.) Dissolving biodegradable polyester polymer, poly(L-lactic acid-Co-caprolactone) copolymer (PLLC) in 1, 1, 1, 3, 3 , in 3-hexafluoro-2-propanol to obtain a polymer solution;

[0020] The biodegradable polyester polymer can also be polycaprolactone, poly(L-lactic acid), poly(D-lactic acid), amorphous poly(D, L-lactic acid) (PDLLA), polyester type Any one of polyurethane, polyethylene terephthalate, polyglycolic acid, poly(D,L-lactic acid-co-glycolic acid), and copolymers of these polymers. The molecular weight of the polyester polymer may not be considered, but it is preferred to use a polyester polymer with a molecular weight ranging from 10,000 to 1,000,000.

[0021] 2.) Using the electrospinning technology to carry out the first electrospinning of the polymer solution prepared above, the operating voltage is 25-45KV, the pinhole diameter is 26G, and the receiver is a platform of 12cm×12cm, and the fiber diameter is obtained. 50-2000nm, porous fibers with a pore d...

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PUM

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Abstract

The invention relates to a preparation technique of a biochemically emulated tissue engineering gullet, which is a bio-active molecule adopts electrical weaving method to weave biodegradalbe polyester polymer for a second time, thus obtaining basement membranes of epithelium similar with gullet and an asymmetric porous nano fiber structure arranged in sequence and similar with muscular layer structure, and the fiber is chemically modified to ingraft the surface of the biochemically emulated tissue engineering gullet and facilitate the growth of cells. The porous nano fiber similar with the basement membranes can support the growth of gullet epithelium, while the oriented fiber arranged in sequence is helpful for inducing the oriented growth of gullet smooth muscle cells, and the porous nano fiber can is cultured for 3 to 4 weeks in DMEM wholly culture medium, thus preparing a biochemically emulated tissue engineering gullet with both epithelium and muscular layer. The gullet of the invention completely simulates the normal gullet tissue of human body in microstructure causing the man made gullet constructed in vitro to be much closer to human gullet in structure and functions, and the ingrafted bio-active molecules are beneficial for retaining the original form and bio-functions of the body cells for the cells ingrafted to the bio-active molecules, and can be used for the restoration and construction of diseased gullet.

Description

technical field [0001] The present invention relates to a preparation method of bionic tissues and organs, in particular to a microscopic structure that completely simulates the normal esophagus of human body to produce bionic tissue engineered esophagus, and the artificial esophagus is placed in the damaged part of the human body to replace the pathological changes Preparation technology of biomimetic tissue engineering esophagus of part of esophagus. Background technique [0002] The normal human esophagus is a hollow tube about 25 centimeters long, connecting the throat and stomach, and mainly plays the role of transporting food and water. The esophagus is composed of mucosa, submucosa, muscle layer and adventitia. The mucosa is composed of non-keratinized stratified squamous epithelial cells, which grow on the basement membrane, which provides the special proteins, growth factors and other nutrients required for cell growth. Substances to regulate cell proliferation, di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/56A61F2/04
Inventor 竺亚斌
Owner NINGBO UNIV
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