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System for on-line desalinization, enrichment and mass spectrum of double split flow noy upgrade liquid chromatogram

A liquid chromatography and desalting technology, which is applied in the field of dual-split nano-upgrade liquid chromatography on-line desalting, enrichment and mass spectrometry systems, can solve the problems of poor separation effect, poor system repeatability, and unfavorable automation, and reaches the application range. Wide, good versatility, strong practicability

Inactive Publication Date: 2008-06-25
徐州脉析医疗技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this method is cumbersome to operate, the repeatability of the system is poor, the separation effect is not good, and it is not conducive to automation
Some people use the method of splitting to inject samples, but it often needs to waste a lot of samples
These limit the use of this method

Method used

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  • System for on-line desalinization, enrichment and mass spectrum of double split flow noy upgrade liquid chromatogram
  • System for on-line desalinization, enrichment and mass spectrum of double split flow noy upgrade liquid chromatogram
  • System for on-line desalinization, enrichment and mass spectrum of double split flow noy upgrade liquid chromatogram

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] see figure 1 , the system consists of a pump 1, a first splitter 2, a second splitter 3, an injection valve 4, a switching valve 5, a back pressure valve 6, a (capillary) separation column 7 and a mass spectrometer 8. The mobile phase transported from the pump 1 can be split through the first splitter 2 and the second splitter 3; the injection valve 4 is connected between the first splitter 2 and the second splitter 3, and the two splitters are respectively Connected to switching valve 5. Finally, the passage leads through the back pressure valve 6 to waste. One end of the capillary separation column 7 becomes tapered after heating and stretching, not only can fix the stationary phase in the separation column directly, but also can be connected with the mass spectrometer; the other end is directly connected with the second splitter 3 .

[0027] When equilibrating the separation column as figure 1 -a, by controlling the switching valve 5, the mobile phase is split at ...

Embodiment 2

[0033] see image 3 , the system consists of pump 1, first splitter 2, second splitter 3, injection valve 4, switching valve 5, back pressure valve 6, (capillary) separation column 7, immobilized metal affinity chromatography monolithic column (IMC) 8 and mass spectrum 9 composition. The mobile phase transported from the pump 1 can be split through the first splitter 2 and the second splitter 3 . The sampling valve 4 is connected between the first flow divider 2 and the second flow divider 3 , and the two flow dividers are respectively connected with the switching valve 5 . Finally, the passage leads through the back pressure valve 6 to waste. One end of the capillary separation column 7 becomes tapered after heating and stretching, not only can fix the stationary phase in the separation column directly, but also can be connected with the mass spectrometer; the other end is directly connected with the second splitter 3 .

[0034] The sample is pumped into the immobilized me...

Embodiment 3

[0037] see figure 1 , the system consists of a pump 1, a first splitter 2, a second splitter 3, an injection valve 4, a switching valve 5, a back pressure valve 6, a (capillary) separation column 7 and a mass spectrometer 8. The mobile phase transported from the pump 1 can be split through the first splitter 2 and the second splitter 3 . The sampling valve 4 is connected between the first flow divider 2 and the second flow divider 3 , and the two flow dividers are respectively connected with the switching valve 5 . Finally, the passage leads through the back pressure valve 6 to waste. One end of the capillary separation column 7 becomes tapered after heating and stretching, not only can fix the stationary phase in the separation column directly, but also can be connected with the mass spectrometer; the other end is directly connected with the second splitter 3 .

[0038] When equilibrating the separation column as figure 1 -a, by controlling the switching valve 5, the mobil...

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Abstract

The invention relates to a double splitflow nano upgrade liquid phase chromatogram online desalting, enrichment and mass spectrum combination system which comprises a pump, a first splitter, a second splitter, a sample valve, a switching valve, a backpressure valve, a capillary tube separation column and a mass spectrometer, wherein, the pump, the first splitter, the sample valve, the second splitter, the capillary tube separation column and the mass spectrometer are connected in series in turn through pipelines; the split ports of the first splitter and the second splitter are respectively connected with the two inlets of the switching valve through the pipelines; moreover, the outlet of the switching valve is connected in series with the backpressure valve and then is communicated with a waste fluid pool through the pipeline. The system has the advantages that: during the sampling and separation of sample, the system has less pressure fluctuation; with small pre-column dead volume, the system overcomes the gradient delay effect caused by large-volume sampling adopted in the prior nano upgrade liquid phase separation system; with excellent separation repeatability, the system is particularly suitable for gradient separation; meanwhile, the system can be used in online desalting, enrichment and mass spectrum combination of sample with high salinity and low concentration; moreover, the system has a simple structure, convenient operation and easily realized automation.

Description

technical field [0001] The invention relates to a micro-separation system, in particular to a dual-split flow nano-upgrade liquid chromatography on-line desalination, enrichment and mass spectrometry system. Background technique [0002] Since the 1980s, with the development of chromatographic theory, people began to realize the advantages of micro-separation systems, and devoted themselves to the research and development of micro-scale, micro-flow high performance liquid chromatography (HPLC) separation systems. In such systems, fused silica capillary tubes, PEEK tubes or PEKSIL tubes with an inner diameter of 20-250 μm are generally used as column tubes, and filled with fillers. Separation of samples at a low flow rate of 0.02-10 μL / min can obtain higher column efficiency and resolution than conventional HPLC systems. At the same time, in such a micro-scale system, the traditional UV detector is no longer suitable due to its short detection optical path and low detection ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/08G01N30/20G01N30/89
Inventor 张丽华段继诚王晖梁作成张维冰梁振张玉奎
Owner 徐州脉析医疗技术有限公司
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