Wood-rotting basidiomycetes for production of ligninolytic enzymes
A technology for lignin-degrading enzymes and basidiomycetes, which is applied in the direction of using microorganisms, enzymes, enzymes, etc., can solve the problems of unsatisfactory yield or production level of lignin-degrading enzymes, and toxic inducers.
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Embodiment 1
[0033] Cerrena unicolor was used as the production strain. First, malt extract agar was inoculated, and then cultured at 27°C for about 10-12 days. Remove 1-2cm from the agar surface 2 The amount of growing mycelium was inoculated in 100ml of nutrient medium in a 500ml Erlenmeyer flask (cultivation time at 27°C was about 5 days). The preculture thus obtained was then homogenized twice for 30 seconds each in a Waring laboratory mixer. Add 50ml of preculture mycelial homogenate per liter of medium in the shake flask, ie add 25ml to a 2L shake flask containing 0.5L of medium containing ethanol production waste. The culture temperature was 27°C; the shaking frequency was 140 rpm. The culture period is 7-10 days. Laccase and manganese peroxidase yields were approximately 50000-150000 IU / L and 500-800 IU / L, respectively.
[0034] The medium composition is as follows:
[0035] a) Pre-medium (g / L):
[0036] Glucose-10.0
[0037] Peptone-0.2
[0038] Yeast Extract - 0.3
[00...
Embodiment 2
[0050] (Repeat the conditions of Example 1; 0.5L main nutrient medium contains 60g / L crushed orange peel).
[0051] Cerrena unicolor was used as the strain. First, malt extract agar was inoculated, and then cultured at 27°C for about 10-12 days. Remove 1-2cm from the agar surface 2 The amount of growing mycelium was inoculated in 100ml of nutrient medium in a 500ml Erlenmeyer flask (cultivation time at 27°C was about 5 days). The preculture thus obtained was then homogenized twice for 30 seconds each in a Waring laboratory mixer. Add 50 ml of preculture mycelial homogenate per liter of medium in the shaker flask, ie add 25 ml to a 2L shaker flask containing 0.5L medium containing crushed orange peel. The culture temperature was 27°C; the shaking frequency was 140 rpm. The culture period is 6-9 days. Laccase and manganese peroxidase yields were approximately 15000-25000 IU / L and 4000-7000 IU / L, respectively.
[0052] The medium composition is as follows:
[0053] a) Pre-...
Embodiment 3
[0068] Trametes versicolor was used as the strain. First, malt extract agar was inoculated, and then cultured at 27°C for about 10-12 days. Remove 1-2cm from the agar surface 2 The amount of growing mycelium was inoculated in 100ml of nutrient medium in a 500ml Erlenmeyer flask (cultivation time at 27°C was about 5 days). The preculture thus obtained was then homogenized twice for 30 seconds each in a Waring laboratory mixer. Add 50 ml of preculture mycelial homogenate per liter of medium in the shaker flask, ie add 25 ml to a 2L shaker flask containing 0.5L medium containing crushed orange peel. The culture temperature was 27°C; the shaking frequency was 140 rpm. The culture period is 4-5 days. Laccase and manganese peroxidase yields were approximately 15000-20000 IU / L and 200-400 IU / L, respectively.
[0069] The medium composition is as follows:
[0070] a) Pre-medium (g / L):
[0071] Glucose-10.0
[0072] Peptone-0.2
[0073] Yeast Extract - 0.3
[0074] K H 2 PO ...
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