Method for extracting tea-polyphenol and separating monomer EGCG from tea

Abstract

The invention discloses a method of extracting tea polyphenol and separating monomer EGCG from tea, which is characterized in that: tea and solid phase alkali are mixed according to mass ratio of 1:0.1 to 2 and grinded jointly and mixed evenly before solved using water, stirred and centrifuged; inorganic acid is added into the supernatant to regulate pH value into 1.0 to 8.0, thereby getting sedimentation and filtering; filter residue is monomer EGCG; the filtrate is tea polyphenol solution which is then dried to get crude product of tea polyphenol. The extraction and separation method adoptsthe mechanical and chemical technique to extract tea polyphenol and separate monomer EGCG. The method of extracting tea polyphenol and separating monomer EGCG from tea has the advantages of new extraction and separation technique without solvent, simple technique, low cost, high yield, high product purity, short production period, no pollution basically and wide industrial prospect for extractingand separating.

Description

A method for extracting tea polyphenols and separating monomer EGCG from tea leaves (1) Technical field The invention relates to a method for extracting tea polyphenols and separating monomer EGCG from tea leaves. (2) Background technology Tea polyphenols are a mixture of polyhydric phenol derivatives extracted from tea leaves, accounting for 13% to 30% of the dry weight of tea leaves, with catechins as the main component, accounting for 60% to 80% of the total phenolic content, mainly composed of Epigallocatechin (EC), gallocatechin (GC), epigallocatechin (EGC), epigallocatechin gallate (EGCG), epicatechin gallate (ECG) composition (structure as follows). R1=R2=H, catechin (C) R1=OH, R2=H, gallocatechin (GC) R1=H, R2=X, catechin gallate (CG) R1=OH, R2=X, gallocatechin gallate (GCG) Tea polyphenol is a kind of natural antioxidant, and it has been widely used in oil, food, medicine, daily chemical, light chemical, cosmetics, health care and many other aspects. ...

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Problems solved by technology

So far, the extraction of tea polyphenols is mainly through organic solvent extraction, metal ion precipitation, resin adsorption, etc., but a large amount of use of organic solvents and metal ions has brought great safety hazards and environmental disasters to production and human beings. To obtain pure catechin monomers or other monomers, further purification is required on the basis of extracted and separated crude products. At present, various chromatographic separation techniques are most commonly used, including column chromatography separation method, high performance liquid phase preparation process, High-speed countercurrent chromatography has the disadvantages of complex operation process, long cycle, difficult control conditions, huge total column load, low preparation effect, easy pollution of chromatographic column, short service life, etc.; and most of industrial production uses toxic solvents such as dichloromethane or Chloroform removes caffeine, which are unfavorable factors in the extraction process of tea polyphenols

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