Novel method for extracting natural vitamin E, phytosterol, fatty acid methyl ester by enzyme catalysis and molecular distillation

A technology of fatty acid methyl esters and natural vitamins, which is applied in the field of phytosterols, molecular distillation to extract natural vitamin E, fatty acid methyl esters, and enzyme catalysis. It can solve the problems of cumbersome process, long time, and poor exchange catalytic effect.

Active Publication Date: 2008-07-23
唐河金海生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since vegetable oil deodorized distillates contain more triglycerides, lipase Novozym435, Lipozyme TL, and Lipozyme RM have good catalytic effects on the esterification of free fatty acids and The catalytic effect of alcohol transesterification is poor, and triglyceride cannot be completely converted into fatty acid ester, which makes it difficult to purify vitamin E, and the obtained vitamin E content is not high
It can be seen from the implementation examples of this patent that this method uses three times of esterification, the time is relatively long, and the process is cumbersome in actual production.
In this method, vitamin E is destroyed by conventional distillation methods.

Method used

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  • Novel method for extracting natural vitamin E, phytosterol, fatty acid methyl ester by enzyme catalysis and molecular distillation

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0041] A method for extracting natural vitamin E from vegetable oil deodorized distillates, comprising the steps of:

[0042] (1) Hydrolysis, add lipase Lipolase-100T in vegetable oil deodorization distillate, enzyme concentration 1.0% (namely the addition of lipase is 1% of distillate weight), add the water of material equivalent, reaction temperature 55 °C, the hydrolysis reaction time is 10 hours.

[0043] (2) Esterification, the material obtained after being processed by step (1), adding mol ratio is the methyl alcohol of 1: 1, adds lipase Novozym435, enzyme concentration 0.5% (being that lipase consumption is vegetable oil deodorization distillate weight 0.5% of ), the reaction temperature was 35° C., and the reaction time was 2 hours.

[0044] (3) Filtration, suction filtration under the condition of a pressure of 0.05MPa, the lipase is separated from the material and can be reused.

[0045] (4) Precipitation, the material from which the lipase is removed by filtration...

example 2

[0051] A method for extracting natural vitamin E from vegetable oil deodorized distillates, comprising the steps of:

[0052] (1) hydrolysis, add lipase Lipolase-100L in vegetable oil deodorization distillate, enzyme concentration 2.0% (that is the add-on of lipase is 2% of distillate weight), material: water is 1: 1, reaction temperature 50°C, reaction time 11 hours.

[0053] (2) Esterification, the material obtained after being processed by step (1), the addition of methanol with a molar ratio of 1: 1.5, the addition of lipase Novozym735, enzyme concentration 1.0% (that is, the amount of lipase is the vegetable oil deodorization distillate weight 1.0%), the reaction temperature is 45°C, and the reaction time is 4 hours.

[0054] (3) Filtration, suction filtration under the condition of a pressure of 0.06MPa, the lipase is separated from the material and reused.

[0055] (4) Precipitation, the material from which lipase B has been removed by filtration is evaporated to reco...

example 3

[0061] A method for extracting natural vitamin E from vegetable oil deodorized distillates, comprising the steps of:

[0062] (1) hydrolysis, add lipase Lipopex-100T in vegetable oil deodorization distillate, enzyme concentration 3.0% (the addition of lipase is 3% of distillate weight), material: water is 1: 1.5, reaction temperature 55°C, reaction time 12 hours, hydrolysis.

[0063] (2) Esterification, after the material obtained by step (1) is processed, add the methyl alcohol that molar ratio is 1: 1.8, add lipase LipozymeTL IM, enzyme concentration 1.5% (being that lipase consumption is vegetable oil deodorization distillate 1.5% by weight), the reaction temperature was 50° C., and the reaction time was 6 hours.

[0064] (3) Filtration, suction filtration under the condition of a pressure of 0.05MPa, the lipase is separated from the material and reused.

[0065] (4) Precipitation, the material from which the lipase is removed by filtration is evaporated to recover excess...

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Abstract

The invention relates to a method to extract a natural vitamin E, a phytosterin, and a fatty acid methyl ester by the enzyme catalysis and the molecular distillation, which is characterized in that a triglyceride in a distillate which is deodorized from a vegetable oil is hydrolyzed into a fatty acid through a lipase, a methanol is catalyzed by the lipase and the fatty acid is esterified and generated into a fatty acid methyl ester, and the natural vitamin E and the phytosterin are produced when the methanol and the fatty acid methyl ester are processed by filtering, desolventizing, freezing and removing the phytosterin, and removing the fatty acid methyl ester through the secondary molecular distillation. The the extraction method of natural vitamin E, phytosterin, and fatty acid methyl este, compared with the prior art, has the advantages of adopting the process that the oil is catalyzed and hydrolyzed first and then the enzyme catalyzes the fat to be esterified and to be processed by the secondary molecular distillation, no producing wastewater, shortening the esterification time, increasing the natural vitamin E content, deploying the secondary freezing and crystallization to remove the phytosterin with high recovery rate of phytosterin, and beneficially increasing the purity of the vitamin E.

Description

technical field [0001] The invention relates to a new method for extracting natural vitamin E, phytosterol and fatty acid methyl ester through enzyme catalysis and molecular distillation. Background technique [0002] There are eight isomers of natural tocopherol, namely α-tocopherol, β-tocopherol, γ-tocopherol, δ-tocopherol and α-tocotrienol, β-tocotrienol, γ-tocotriene Phenol, delta-tocotrienol. The eight isomers of natural vitamin E all have optical activity. Among them, the optical activity of (RRR)-α-tocopherol is very small. Specific rotation (in 2,2,4-trimethylpentane) is only +0.16°, specific rotation in ethanol [α] 25 546.1 is +0.32°. Specific rotation of β-tocopherol [α] 25 546.1 is +2.9° (c=7.15 in ethanol). Specific rotation of γ-tocopherol [α] 25 546.1 is +2.2° (c=9.32 in ethanol). δ-tocopherol Specific Rotation [α] 25 546.1 is +3.4° (c=15.5 in ethanol). [0003] Natural vitamin E is a light yellow viscous transparent liquid, odorless and tasteless...

Claims

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Application Information

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IPC IPC(8): C12P7/62C12P33/00
Inventor 谷克仁肖咏梅王宏雁江秀明夏萍郑丽梅王秀花
Owner 唐河金海生物科技有限公司
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