The invention discloses a method for preparing sulfated apple
pomace polysaccharide through twin-screw
extrusion, and belongs to the technical field of
food processing. The method comprises the following steps: (1) carrying out
extrusion modification on apple
pomace subjected to
moisture adjustment by adopting a double-screw extruder, and performing
drying, crushing and sieving to obtain modifiedapple
pomace; (2) adding
distilled water into the modified apple pomace, performing leaching in a water bath, removing filter residues, concentrating under reduced pressure, carrying out
alcohol precipitation, standing, centrifuging, removing supernatant, and
drying the precipitate to obtain apple pomace
polysaccharide; and (3) dispersing the apple pomace
polysaccharide in
dimethylformamide, adding
sulfamic acid under a stirring condition, uniformly mixing, carrying out
esterification reaction under an ultrasonic condition, cooling to
room temperature, regulating the pH value until the solution is neutral, carrying out
alcohol precipitation, dissolving the obtained precipitate in
distilled water, performing dialyzing, and
drying the obtained product to obtain the sulfated apple pomace polysaccharide. The method has the advantages of high production efficiency, high yield, safety and no
toxicity of the product, and high yield of the obtained product.