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Method for preparing loach selenium-rich active peptide nutrient solution or nutrition powder

A technology of active peptide and nutrient solution, which is applied in the fields of bioengineering and membranes, can solve the problems of deep processing and comprehensive utilization of loach, and achieve the effects of relative molecular quality control, increased yield, and improved functionality

Inactive Publication Date: 2008-08-06
JIANGNAN UNIV
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AI Technical Summary

Problems solved by technology

[0005] Because of the rich nutritional value of loach, the development of health care products and medicines using loach as raw material has just begun at home and abroad. There are only a small amount of rough processing patents and research reports, and deep processing and comprehensive utilization of loach have not yet been possible.

Method used

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  • Method for preparing loach selenium-rich active peptide nutrient solution or nutrition powder

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Embodiment 1

[0024] Embodiment 1 Preparation of degree of hydrolysis (DH)=18% loach selenium-enriched active peptide nutrient solution and nutrient powder

[0025] The loach meat is mashed to obtain a homogenate, and water is added in a ratio of 1:1, and acid protease is used to carry out enzymolysis reaction at pH 3.0-4.0 and 40°C. The enzyme dosage is 1%-3% of the total weight of the loach homogenate and water, Enzymolysis for 2-10 hours, using the TNBS method to control the degree of hydrolysis at 18%, heating to inactivate the enzyme after the reaction, and after centrifugal filtration, the filtrate was desalted and concentrated with a polyamide nanofiltration membrane with a molecular weight cut-off of 150Dalton provided by Dow Chemical Company of the United States. During the desalination process, water is continuously added to dilute the feed liquid, and the volume of the concentrated liquid is kept constant. After desalination, the feed liquid is further concentrated to obtain the ...

Embodiment 2

[0032] Embodiment 2 Preparation of loach selenium-enriched active peptide nutrient solution and nutrient powder with degree of hydrolysis (DH)=20%

[0033] The loach meat is mashed to obtain a homogenate, and water is added at a ratio of 1:1, and the alkaline protease Alcalase is used to carry out enzymolysis reaction at 60°C and pH 8.5, and the enzyme dosage is 1%-3% of the total weight of the loach homogenate and water , enzymatic hydrolysis for 2-10 hours, using the pH-STAT method to control the degree of hydrolysis at 20%, heating to inactivate the enzyme after the reaction, and after centrifugal filtration, the filtrate is desalted and concentrated with a nanofiltration membrane with a molecular weight cut-off of 300Dalton, and water is continuously added during the desalination process Dilute the feed solution and keep the volume of the concentrate unchanged. After desalination, the feed solution is further concentrated to obtain the loach selenium-enriched active peptid...

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Abstract

The invention relates to a method for preparing selenium-rich active peptide loach nutrient solution or powder, pertaining to bioengineering and membrane technology field. Enzymatic hydrolysis-nanofiltration concentration technique is adopted in the preparation method of the selenium-rich active peptide loach nutrient solution or powder in the method for preparing the selenium-rich active peptide loach nutrient solution or the powder; loach is utilized as material and water in a proportion of 1: 1 is added; enzymolysis reaction is caused in case of pH 3-10 of protease and at a temperature of 30 to 60 DEG C, the dosage of protease is 1 to 3 percent of the total weight of uniform loach slurry and water and the enzymolysis reaction lasts for 2 to 10 hours; the degree of hydrolysis is controlled between 12 to 20 percent through a pH-STAT or TNBS method; after the completion of the reaction, the protease is inactivated and the obtained solution is centrifugally filtered to obtain the enzymolysis solution which is concentrated and desalted with a nanofiltration membrane so that the selenium-rich active peptide loach nutrient solution is prepared, which can be sprayed and dried to obtain the selenium-rich active peptide loach nutrient powder. The method for preparing the selenium-rich active peptide loach nutrient solution or the powder has the advantages of large processing quantity, moderate separation conditions, simple process and low energy consumption, and can be scale manufactured.

Description

technical field [0001] A method for preparing loach selenium-enriched active peptide nutrient solution or nutrient powder, specifically relates to the method of enzymatic hydrolysis and nanofiltration membrane concentration and desalination, using loach protein to prepare loach selenium-enriched active peptide nutrient solution or nutrient powder, which belongs to bioengineering and membrane technology. Background technique [0002] Loach is known as "ginseng in water", delicious taste, high nutritional value, protein, sugar, minerals (calcium, phosphorus, iron) and vitamins (V B , V A , V C ) were higher than that of common fish and shrimp. The protein content reaches 18 grams per 100 grams of loach, and the content of organic selenium reaches 35 micrograms per 100 grams of loach. Since ancient times, loach has been considered to have high medicinal value. According to the investigation in "Introduction to Medicine", loach is sweet and flat in nature, and has the funct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/327A23L1/305A23L2/38A23P1/02A23L33/18A23P10/28
Inventor 杨瑞金卢蓉蓉张文斌
Owner JIANGNAN UNIV
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