Method for preparing collagen peptide

A fish scale collagen and fish scale technology, which is applied in the biological field, can solve the problems of inability to fully utilize fish resources and waste, and achieve the effects of high absorption and utilization rate, good acid resistance and high temperature resistance.

Inactive Publication Date: 2008-08-13
湖北瑞邦生物科技有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

At present, the traditional fish processing industry can no longer realize the full utilization of fish resources. A large number

Method used

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  • Method for preparing collagen peptide

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Embodiment

[0018] 1) Bacillus subtilis CICC10071 is expanded and cultivated, and the bacterial cell content in the seed liquid is 10 8 per ml, considered as mature seeds.

[0019] 2) After cleaning the fish scales and removing impurities, add 8 times the weight of pure water to cook at 100°C for 30 minutes, and then enter the colloidal grinding slurry. The slurry passes through a 60-mesh screen and enters the fermenter at 121°C for 30 minutes for high-temperature sterilization , ready to inoculate and ferment after cooling.

[0020] 3) Inoculate the mature seeds with 5% of the volume of the fish scale slurry, heat-preserve and ferment for 60 hours at 35°C, sterilize at 121°C for 10 minutes, cool to 45°C-50°C, separate and remove the slag, and take the supernatant for preparation.

[0021] 4) The supernatant is separated by a membrane system, the macromolecules with a molecular weight of 5000 or more are intercepted, and the small molecular liquid with a molecular weight of 5000 or less ...

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Abstract

The invention provides a method for preparing fish scale collagen protein peptides comprising the following steps (1) preparing fermentation seeds culture medium, (2) preparing fermentation seeds, (3) processing fish scale, (4) fermenting with constant temperature, (5) separating, (6) delicacy, (7) drying to obtain fish scale collagen protein peptides powder. The preparing process incorporating the invention comparing with other methods is simple, prepared collagen protein peptides can be solved into water complete, its solution in water is low viscosity and also has good flow property in high concentration, has good acid resistant and has no precipitating in acid or alkaline circumference, also has good high temperature resistant which has no precipitating even it is heated up to 100 DEG C.

Description

(1) Technical field [0001] The invention relates to a biotechnology, in particular to a method for preparing fish scale collagen peptides by means of biotechnology. (2) Background technology [0002] Collagen peptide is an important functional protein. It is a hydrolyzed product of collagen. It has physical characteristics such as low molecular weight, easy absorption, and low viscosity. It is related to cell proliferation, differentiation, exercise, immunity, joint lubrication, wound healing, etc. are closely related and have strong biological activity. Due to the special functions of collagen peptides, its extracts have been widely used in industrial fields such as medicine, food, daily chemical industry, and biosynthesis. At present, the raw materials for the production of collagen products are basically the skin and bones of terrestrial animals such as pigs and cattle. Due to the deterioration of the living environment of terrestrial animals and the occurrence of mad co...

Claims

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Application Information

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IPC IPC(8): C12P21/06C12R1/125
Inventor 陈成滕春红
Owner 湖北瑞邦生物科技有限公司
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