Levulose group fermentation raw material and uses thereof
A fermented raw material, fructose-based technology, applied in the field of fructose-based fermented raw materials and its application
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Embodiment 1
[0015] Dry the tubers of Jerusalem artichoke, crush them into powder through a pulverizer, and sieve through 50-200 mesh to make Jerusalem artichoke powder.
Embodiment 2
[0017] After dissolving the Jerusalem artichoke powder prepared in Example 1 with 55°C water to make a 10% solution, adjust its pH value to 6.5 through 1M NaOH and 1M HCl, and prepare the enzymatic hydrolysis of Jerusalem artichoke powder at the ratio of adding 10000U of inulinase per liter The reaction solution (inulinase enzyme activity is defined as the amount of enzyme needed to hydrolyze the substrate to produce 1 micromole of fructose per minute) was reacted in the reactor at 45°C for 9 hours, and was detected by HPLC. After the enzymatic hydrolysis reaction was complete, fructose Jerusalem artichoke powder liquid (glucose 0.9%, fructose 9.1%) is spray-dried to make fructified Jerusalem artichoke powder.
Embodiment 3
[0019] After the Jerusalem artichoke powder prepared in Example 1 was dissolved in 70°C water to make a 20% solution, the pH value was adjusted to 4.2 by 1M NaOH and 1M HCl, and the enzymatic hydrolysis of Jerusalem artichoke powder was prepared by adding 50,000 U units of inulinase per liter The reaction solution (inulinase enzyme activity is defined as the amount of enzyme needed to hydrolyze the substrate to produce 1 micromole of fructose per minute) was reacted in the reactor at 70°C for 5 hours, and was detected by HPLC. After the enzymatic hydrolysis reaction was complete, fructose Jerusalem artichoke powder liquid (glucose 1.4%, fructose 16.6%) is spray-dried to make fructified Jerusalem artichoke powder.
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