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Levulose group fermentation raw material and uses thereof

A fermented raw material, fructose-based technology, applied in the field of fructose-based fermented raw materials and its application

Inactive Publication Date: 2008-08-20
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limited area of ​​cultivated land in my country, my country produces 120 million tons of corn annually, which is less than half of that of the United States. The per capita share is less than 100 kilograms, and the consumption of breeding accounts for 70-80%. The corn used for industry accounts for 10% of the total corn production. About 10 million tons per year, therefore, the domestic cornstarch is far from being able to meet the development of large-scale fermentation industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Dry the tubers of Jerusalem artichoke, crush them into powder through a pulverizer, and sieve through 50-200 mesh to make Jerusalem artichoke powder.

Embodiment 2

[0017] After dissolving the Jerusalem artichoke powder prepared in Example 1 with 55°C water to make a 10% solution, adjust its pH value to 6.5 through 1M NaOH and 1M HCl, and prepare the enzymatic hydrolysis of Jerusalem artichoke powder at the ratio of adding 10000U of inulinase per liter The reaction solution (inulinase enzyme activity is defined as the amount of enzyme needed to hydrolyze the substrate to produce 1 micromole of fructose per minute) was reacted in the reactor at 45°C for 9 hours, and was detected by HPLC. After the enzymatic hydrolysis reaction was complete, fructose Jerusalem artichoke powder liquid (glucose 0.9%, fructose 9.1%) is spray-dried to make fructified Jerusalem artichoke powder.

Embodiment 3

[0019] After the Jerusalem artichoke powder prepared in Example 1 was dissolved in 70°C water to make a 20% solution, the pH value was adjusted to 4.2 by 1M NaOH and 1M HCl, and the enzymatic hydrolysis of Jerusalem artichoke powder was prepared by adding 50,000 U units of inulinase per liter The reaction solution (inulinase enzyme activity is defined as the amount of enzyme needed to hydrolyze the substrate to produce 1 micromole of fructose per minute) was reacted in the reactor at 70°C for 5 hours, and was detected by HPLC. After the enzymatic hydrolysis reaction was complete, fructose Jerusalem artichoke powder liquid (glucose 1.4%, fructose 16.6%) is spray-dried to make fructified Jerusalem artichoke powder.

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PUM

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Abstract

The invention relates to a fructosyl fermented raw material and the application, 1) an inulin-rich organ of an inulin plant is dried, smashed into powder by a smashing machine, screened by 50 to 200 meshes and produced into powder; 2) the powder is dissolved by water at 45 to 70 DEG C to be made into the solution with a weight concentration of 10 to 20 percent, an inulin powder enzymolysis reaction liquid is prepared according to the proportion of each liter solution added with 1000U to 100000U units of inulinase, the pH value is regulated to 4.2 to 6.5, the reaction is carried out in a reactor at 45 DEG C to 70 DEG C for 3 to 9 hours, a fructose inulin powder liquid is formed after the enzymolysis reaction, or the fructose inulin powder is prepared by further spray drying. The production cost is low, the sources of the raw material are wide, the technique is simple, the technical route is mature and the industrial implementation can be realized.

Description

technical field [0001] The invention relates to a fructose-based fermentation raw material prepared from inulin plants as a fermentation substrate instead of starch fermentation raw materials such as corn starch, which is applied to the production process of food, fuel, chemicals, materials and biological preparations in the fermentation industry. Background technique [0002] Starch is the most simple and easy-to-obtain glucose-based raw material, and it is also very easy for microorganisms to use. At present, my country's starch is mainly made from corn. However, due to the limited area of ​​cultivated land in my country, my country produces 120 million tons of corn annually, which is less than half of that of the United States. The per capita share is less than 100 kilograms, and the consumption of breeding accounts for 70-80%. The corn used for industry accounts for 10% of the total corn production. About, only more than 10 million tons per year, therefore, the domestic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/00C12P7/00C12P7/02C12P7/40C12P13/04C12P19/02C12P21/02C12C11/00C12J1/00C12N9/00
Inventor 杜昱光周正曹海龙李曙光曲天明
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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