Transgenic cell overlapped vascular inner rack and manufacture method thereof

Abstract

The invention provides an intravascular stent covered by transgene cells, which is characterized in that: a low-temperature plasma treatment layer, an adhesive interstitial substance coat and a cell coat are orderly arranged on the surface of the naked metal stent. The adhesive interstitial substance coat is a protein coat or a peptide sequence coat; the cell coat is obtained by stabilizing transfected cells by target gene. Preparing the intravascular stent includes the following steps: obtaining the target gene; constructing and amplifying the eukaryotic expression carrier of the target gene; obtaining cells; modifying the surface of the metal stent; preparing the adhesive interstitial substance coat; rotating, cultivating, and preparing the intravascular stent covered by transgene cells. On the one hand, by expressing the target gene at high-level, the rehabilitating of the injured endovascular endoderm is enhanced and the multiplication of smooth muscle cells is inhibited; one the other hand, by the multiplication of external-source cells, the surface endothelialization of the intravascular stent is accelerated; the coating is close applied on the surface of the stent, the biocompatibility is fine, and the intravascular stent can be inhibited from turning narrower ultimately.

Description

technical field [0001] The invention relates to a transgenic cell-covered intravascular stent, in particular to a transgenic cell-covered intravascular stent for inhibiting restenosis after percutaneous coronary angioplasty or stent implantation and a preparation method thereof. Background technique [0002] Intravascular restenosis is mainly due to mechanical damage (mainly endothelial layer damage) to the vessel wall caused by interventional surgery, which causes endothelial cell exfoliation and dysfunction, resulting in long-term exposure of the damaged part to the blood environment, inducing autoimmune stress and inflammatory response , inducing the migration and proliferation of monocytes in the blood and smooth muscle cells in the vascular media to the intima, and finally forming restenosis. Currently, restenosis has become a major challenge and research hotspot in vascular interventional therapy. [0003] Vascular endothelial cells are a class of cells with complex f...

AI Technical Summary

Problems solved by technology

It is clinically required that the simpler the operation of implanting endothelial cells and the shorter the time, the better. Therefore, primary vascular endothelial cells obtained from body veins alone cannot meet the requirements of implantation.

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