Common camellia flavone glycosides A with estrogen liveness, preparation method and application thereof
A technology of camellia flavonoid glycosides and estrogens is applied in the directions of organic active ingredients, medical preparations containing active ingredients, organic chemistry, etc., and can solve the problems of little medicinal value of camellia plants.
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[0031] The present invention will be further described below in the form of specific examples. Unless otherwise specified, mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR) analysis techniques ( 1 H-NMR, 13 C-NMR, H-H COZY, HMBC, HMQC) to analyze the material structure.
[0032] One, the preparation of camellia flavonoid glycoside A
[0033] ①Preparation of ethyl acetate extract of Nanshan tea seed (Camellia semiserrata Chi.) oil meal: heat and reflux extraction with 95% industrial ethanol, solid-liquid ratio 1:10 (g:ml), temperature 95°C, extract 3 times, each time 3 hours; the ethanol extract was concentrated into an extract, suspended with 3 times distilled water, and extracted 3 times with 3 times petroleum ether and 3 times ethyl acetate in sequence according to the volume ratio, to obtain ethyl acetate extract;
[0034] ② Separation of the above-mentioned ethyl acetate extract by silica gel column chromatography, chloroform:methanol 15:1 (volume) mixed s...
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