Glass carbon electrode decorated by carbon nano tube-DNA complex and its production method and application

A carbon nanotube and composite technology, which is applied in the application field of carbon nanotube-DNA composites, can solve the problems of poor conductivity of multi-walled carbon nanotubes, limited detection efficiency, small specific surface area, etc., and achieves good detection effect and method. The effect of simple process and simple preparation process

Inactive Publication Date: 2008-09-24
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the conductivity of multi-walled carbon nanotubes is relatively poor, it is not easy to disperse on the surface of the electrode, and the specific surface area is relatively small, which limits the detection efficiency

Method used

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  • Glass carbon electrode decorated by carbon nano tube-DNA complex and its production method and application
  • Glass carbon electrode decorated by carbon nano tube-DNA complex and its production method and application
  • Glass carbon electrode decorated by carbon nano tube-DNA complex and its production method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Take 1mL (GT) 20 Single-stranded DNA aqueous solution (concentration: 1 mg / mL), weighed 1 mg of single-walled carbon nanotubes and put them in the solution, dispersed in an ice-water bath with 100 W ultrasonic for 2 hours, and controlled the temperature below 4°C. The obtained mixture was centrifuged at a high speed of 10000 r / min for 2 hours, the precipitate was filtered off, and the supernatant was taken to obtain a carbon nanotube-DNA solution. The carbon nanotube solution is frozen at the temperature of liquid nitrogen (put the test tube containing the carbon nanotube solution into a liquid nitrogen container and freeze-dry for 20 minutes); then in the frozen state, put the carbon nanotube solution into a rotary evaporator , and dried for 24 hours to obtain powdery carbon nanotube powder. Then 0.5 g of the powder was ultrasonically dispersed in 500 μL of ethanol to obtain a solution of carbon nanotubes dispersed in ethanol. The glassy carbon electrode (Φ3mm) was s...

Embodiment 2

[0023] Take 1mL (GT) 60 For single-stranded DNA solution (0.5 mg / mL), weigh 2.0 mg of single-walled carbon nanotubes and place them in the solution, and ultrasonicate at 50 W for 3 hours in an ice-water bath, with the temperature controlled below 4°C. The obtained mixture was centrifuged at a high speed of 15000 r / min for 1 hour, the precipitate was filtered off, and the supernatant was taken to obtain a carbon nanotube-DNA solution. Freeze the carbon nanotube solution at liquid nitrogen temperature (put the test tube containing the carbon nanotube solution into a liquid nitrogen container and freeze-dry it for 20 minutes); then put the carbon nanotube solution into a rotary evaporator in a frozen state , and dried for 24 hours to obtain powdery carbon nanotube powder. Then 0.5 g of the powder was ultrasonically dispersed in 500 μL of ethanol to obtain carbon nanotubes dispersed in ethanol. The glassy carbon electrode (Φ3mm) was successively coated with 0.1μm, 0.05μm Al 2 o...

Embodiment 3

[0025] Take 1mL (GT)60 For single-stranded DNA solution (1.5 mg / mL), weigh 1.0 mg of multi-walled carbon nanotubes and place them in the solution, ultrasonicate at 200 W for 1 hour in an ice-water bath, and control the temperature below 4°C. The obtained mixture was centrifuged at a high speed of 18000 r / min for 0.5 hour, the precipitate was filtered off, and the supernatant was taken to obtain a carbon nanotube-DNA solution. The carbon nanotube solution is frozen at the temperature of liquid nitrogen (put the test tube containing the carbon nanotube solution into a liquid nitrogen container and freeze-dry for 20 minutes); then in the frozen state, put the carbon nanotube solution into a rotary evaporator , and dried for 24 hours to obtain powdery carbon nanotube powder. Then 0.5 g of the powder was ultrasonically dispersed in 500 μL of ethanol to obtain carbon nanotubes dispersed in ethanol. The glassy carbon electrode (Φ3mm) was successively coated with 0.1μm, 0.05μ Al 2 o...

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Abstract

The invention discloses a modifying glassy carbon electrode with a carbon nanotube-DNA complex, a preparation method and an application thereof. The modifying glassy carbon electrode with the carbon nanotube-DNA complex is formed by coating a carbon nanotube-DNA complex film at the outside of a glassy carbon electrode substrate. The process of the preparation method is that a carbon nanotube and water solution of single stranded DNA which only contains G and T are mixed to prepare carbon nanotube-DNA mixed liquid, the carbon nanotube-DNA solution is prepared by the ultrasonic breaking mixed treatment and the centrifugal separation, the powder carbon nanotube-DNA complex is obtained by freeze drying, and the modifying glassy carbon electrode with the carbon nanotube-DNA is prepared by coating the carbon nanotube-DNA complex film formation on the glassy carbon electrode. The prepared modifying glassy carbon electrode with the carbon nanotube-DNA has application in the detection of the concentration of hydrogen peroxide solution. The preparation method has the advantage that the preparation process is simple, which is particularly easy to realize the detection of the concentration of the hydrogen peroxide solution in a biological body.

Description

technical field [0001] The invention relates to a glassy carbon electrode modified by a carbon nanotube-DNA compound, a preparation method and an application thereof, and belongs to the application technology of the carbon nanotube-DNA compound. Background technique [0002] Hydrogen peroxide is the product or intermediate product of many biochemical reactions, and is related to many biological processes. By measuring hydrogen peroxide, the content of many substrates or enzymes can be determined indirectly. At the same time, hydrogen peroxide is also an important chemical product, which is widely used in industries such as textile, paper making, chemical industry, electronics, light industry, and sewage treatment. Traditional hydrogen peroxide detection techniques such as chromatography, colorimetry, titration, UV-visible spectroscopy, and chemiluminescence are generally time-consuming, susceptible to interference, and difficult to detect automatically. How to continuously ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/36G01N27/327
Inventor 杨全红向东亚王琪
Owner TIANJIN UNIV
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