Substrate with spatially selective metal coating, method for production and use thereof
A metal coating, selective technology, used in metal material coating process, coating, liquid chemical plating and other directions
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Embodiment 1
[0048] The surface is covered with metal clusters by controlled plating with a biological template (S-Layer-Patches of Bacillus sphaericus NCTC 9602).
[0049] The preparation of the S-layer is based on the publication of Engelhard H.; Saxton, W.; Baumeister, W. "Three-dimensional structure of tetragonal surface layer of Sporosarcinaurea", J. Bacteriol. 168(1), 309, 1986 get on. Standard buffer used to store insulated and purified S-layers at 4°C by adding 3mM NaN 3 And 1mMMgCl 2 It is composed of 50mM tris(hydroxymethyl)aminomethane hydrochloride (TRIS / HCl) solution.
[0050] The S-layer solution for all other experiments is based on the standard with a concentration of 10 mg / ml.
[0051] This preamplifier is 3mM K for at least 24 hours 2 PtCl 4 The solution, based on the calculation used to cover the protein with metal clusters, was spiked with 13 μl of protein solution. The alternating action between the S-layer solution and the metal complex solution was carried out under inci...
Embodiment 2
[0054] As in Example 1, the bio-template was plated in a controlled manner, but first, the protein monomer was recrystallized on each substrate to cover the surface with metal clusters in a targeted manner.
[0055] The S-layer solution used in accordance with the standard was freeze-dried and then suspended in a 0.8 M TRIS buffered guanidine hydrochloride solution, so that the final concentration of the protein solution was 10 mg / ml. After 30 minutes of alternating action time between reagents, transfer the solution to a prepared dialysis hose (VISKING Typ 27 / 32) or dialysis room, and use water and subsequently MgCl-free 2 The standard buffer for dialysis. After this step, the solution present in the dialysis hose was transferred to an appropriate reaction vessel, and centrifuged at 4°C and 20000 g for 10 minutes. The pellets formed after this step are discarded, and the remaining monomer solution is used for subsequent operations (the monomer solution can be kept for about 5 day...
Embodiment 3
[0059] Through controlled plating with biological templates (S-Layer-Patches of Bacillus sphaericus NCTC 9602 (Bacillus sphaericus NCTC 9602)), and avoid the use of chloride and hydrazine, targeted Cover the surface of exhaust gas catalysis with noble metal clusters.
[0060] The preparation of the S-layer is based on Engehard H.; Saxton, W,; Baumeister, W. "Three-dimensional structure of tetragonal surface layer of Sporosarcinaurea", J. Bacteriol. 168(1), 309, 1986. To proceed. Standard buffer used to store (at 4°C) insulated and purified S-layers, by adding 3mM NaN 3 And 1mMMgCl 2 It is composed of 50mM tris(hydroxymethyl)aminomethane hydrochloride (Tris / HCl) solution.
[0061] The S-layer solution for all other experiments is based on the standard with a concentration of 10 mg / l.
[0062] Alumina particles (per 100 mg) were mixed with 825 μl of active S-layer solution and kept for 24 hours for alternating action. After that, use distillation H twice 2 O rinse.
[0063]Add 10.83...
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