Tobacco and method for separating liquid phase chromatography of amino acid contained in product

A technology of liquid chromatography and separation method, applied in the field of liquid chromatography separation of amino acids contained in tobacco and its products, can solve problems such as no chromatographic separation method, and achieve the effect of good chromatographic peak shape

Active Publication Date: 2008-10-01
CHINA TOBACCO GUANGDONG IND
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  • Abstract
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Problems solved by technology

[0006] There is no report about the complete technical scheme of the above-mentioned chromatographic separation method at present

Method used

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  • Tobacco and method for separating liquid phase chromatography of amino acid contained in product
  • Tobacco and method for separating liquid phase chromatography of amino acid contained in product
  • Tobacco and method for separating liquid phase chromatography of amino acid contained in product

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Embodiment 1

[0024] 1. Instruments, materials and reagents

[0025] LC-MS LTQ Liquid Chromatography-Ion Trap Mass Spectrometer from Thermo Electric Company, equipped with autosampler, quaternary gradient mass spectrometer pump, LTQ ion trap mass spectrometer charged spray ionization source (ESI), Xcalibur 1.4SR1 system control software; Italy Claind N2LCMS Nitrogen generator; French Millipore Element A10 pure water machine; American Cole-Parmer 8892 ultrasonic cleaner; Sartorious CP225D electronic balance (Max 220g, d=0.01mg (80g), 0.1mg (220g)); Whatman 0.2μm nylon needle type filter; tobacco powder. Mixed amino acid standard solution (Fluka Company), containing 17 kinds of amino acids, the concentration is 1mM each; internal standard L-norvaline (Shanghai Livzon Dongfeng Biotechnology Co., Ltd.); asparagine water (Shanghai Boao Biotechnology Co., Ltd. Co., Ltd.); tryptophan, proline and glutamine (Sinopharm Chemical Reagent Co., Ltd.); γ-aminobutyric acid (Sigma); L-piperidine-2-carboxy...

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Abstract

The present invention discloses a method for separating a liquid chromatogram of the amino acid contented in tobacco and the product thereof. The method comprises the procedures of preparing an extraction liquid of the sample to be measured; and taking an A phase 99%water-1%ethane nitrile-0.1%nonafluoro valeric acid and a B phase 10%water-90%ethane nitrile-0.1%nonafluro valeric acid as a flowing phase gradient for elution and separation on the HyPURITY C18 reverse-phase chromatographic column. Aiming at the disadvantage of the method for analyzing the content of amino acid contended in tobacco and the product thereof, the invention accurately limits the condition of the liquid chromatogram analysis and provides an integral and whole experiment plan for obtaining an excellent chromatographic peak shape and an appropriate color spectrum runtime. Furthermore, the isomeride of the amino acid can be facilitated to obtain a base line separation. The chromatogram separation method provided by the invention can obtain an accurate analyzing result to the content of the amino acid in tobacco and the product thereof when combined with a mass spectrometric detection.

Description

technical field [0001] The invention belongs to the technical field of chemical analysis and detection, and in particular relates to a liquid chromatography separation method for amino acids contained in tobacco and its products. Background technique [0002] Amino acids are important components in tobacco leaves, which are closely related to smoke quality and cigarette brand characteristics; amino acids participate in the non-enzymatic brown reaction in tobacco leaves, namely the Maillard reaction, which can produce a variety of important aroma components. Therefore, the study of amino acids in tobacco is of great significance for formula development, process production and quality control. [0003] Because there are many kinds of amino acids in tobacco, and they have three different chemical properties: acidic, neutral and basic, and the matrix of tobacco is complex, it is difficult to simultaneously measure multiple amino acids in tobacco. Currently, liquid chromatograph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/89
Inventor 黄翼飞
Owner CHINA TOBACCO GUANGDONG IND
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