Method for extracting main active components of mulberry leaf and application of its extract
The technology of a mulberry leaf extract and an extraction method is applied in the fields of phytochemistry, the modernization of traditional Chinese medicine, and the prevention and treatment of diabetes, and can solve the problems of incomplete extraction and utilization of mulberry leaf active components, cumbersome technological process, inability to separate effective components, etc. The effect of simple preparation process and easy operation
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Embodiment 1
[0023] Mulberry leaves 10kg, cut into pieces, boiled in water for 30 minutes to extract twice, 50L each time. The extract was left to cool to room temperature, and the supernatant was passed through a D72 resin column (column volume 10L), washed with 10L of water and then eluted with 20L of ethanol, and then eluted with 30L of 1% ammonia / ethanol water (containing 70% ethanol) solution . After the ammonia water ethanol eluent is recovered to be free of ammonia, the previous 20L ethanol eluent is added to recover to a volume of 3L to obtain a liquid extract.
[0024] After most of the water was evaporated to 3L, the resin was precipitated by adding 7L of ethanol. The supernatant was removed by centrifugation, mixed with the liquid extract above, vacuum-dried, and pulverized to obtain 300 g of yellow powder with a yield of 3%.
[0025] Get 100g of mulberry leaf extract, add starch 3000g, microcrystalline cellulose 30g, granulate with 50% ethanol, compress into tablets to obtain...
Embodiment 2
[0027] 10kg of mulberry leaves, cut into pieces, boiled with 50% ethanol / water for 30 minutes to extract twice, 50L each time. After the extract was concentrated to recover ethanol, it was diluted with 100 L of water, and then passed through a D72 resin column (column volume 10 L). According to the method described in Example 1, 400 g of yellow powder was obtained with a yield of 4%, which was the mulberry leaf extract referred to in the present invention.
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