Enteric oral vaccine for fishing gear and preparation method thereof

Abstract

The invention relates to an enteric-coated oral vaccine for fishing, belonging to the field of oral vaccines for fishing. The enteric-coated oral vaccine for fishing comprises particles or pellets sprayed with a vaccine layer and sequentially coated with an isolating layer and an enteric-coated layer outside the vaccine layer. A dry powder composition A for spraying vaccine and coating the isolating layer comprises the following components (by weight parts) one or more film-forming agents selected from hydroxypropyl methylcellulose, polyvinyl alcohol and polyvinyl pyrrolidone 40-60 parts, one or more plasticizing agents selected from phosphoglyceride, triethyl citrate and polyethylene glycol 3-10 parts, one or more antisticking agents selected from silicon dioxide, magnesium stearate, talcum powder and titanium oxide 30-50 parts, and surfactant selected from lecithin and Tween-80 0-5 parts. The vaccine can be directionally released in intestinal tract.

Description

technical field [0001] The invention belongs to the field of fish oral vaccines. Background technique [0002] Diseases of farmed fish are increasing year by year, causing huge economic losses and seriously hindering the development of aquaculture. At present, the main means of controlling various diseases is extensive and large-scale input of chemical drugs and antibiotics. As a result of long-term and large-scale use of chemical drugs and antibiotics, environmental pollution problems, pathogenic and non-pathogenic microbial resistance problems, As well as food safety issues with excessive drug residues; for example, the turbot incident is a typical example, which has attracted close attention from many parties. At present, the state has banned the production and use of seriously harmful drugs. Improving the ability to prevent and control diseases in aquaculture is imminent. With the requirements of green food and environmental protection and the development of immunolog...

AI Technical Summary

Problems solved by technology

However, the above technologies have not carried out research on the physiological characteristics of the gastrointestinal tract of fish, nor have they carried out enteric test observations, and cannot establish a suitable vaccine controlled release technology. Identify the release site of microparticle and microcapsule vaccines in the gastrointestinal tract

The materials used in its preparation technology do not have a definite pH dependence, can only achieve oral delivery and exert a certain sustained release effect, and cannot effectively achieve the purpose of intestinal release. If the vaccine is released too early, it will be digested by gastric acid and digestive enzymes , destroy, and cannot effectively protect the vaccine from acting on the immune-related lymphoid tissue of the intestinal mucosa

Moreover, in the preparation process of the above technology, the vaccine needs to be miscible into the organic solvent of the polymer material. The existence of the organic solvent will destroy the immunogenicity of the vaccine, resulting in unstable immune effect of the vaccine, which further limits its application in production.

[0005] Due to the influence of gastrointestinal factors, most protein drugs are easily inactivated and poorly absorbed, resulting in poor bioavailability, and their oral drug delivery system still faces challenges

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