Liquid bacterial culture medium for industrial preparation of hypsizygus marmoreus and preparation method thereof

Abstract

The invention provides a liquid strain culture medium for Lyophyllum shimeji industrial production and a preparation method thereof. The liquid strain culture medium per liter contains 2-5g of soybean cake powder, 2-5g of maize meal, 20-30g of sucrose, 0.3-1g of magnesium sulfate, 0.5-2g of potassium dihydrogen phosphate, and 0.1-0.5 ml of defoaming agent. The preparation method comprises weighing the sucrose, the magnesium sulfate and the potassium dihydrogen phosphate, mixing completely, adding boiling water, and stirring to dissolve completely; weighing the soybean cake powder and the maize meal, adding cold water, stirring to dissolve, and removing bubbles; mixing them together to obtain a mixed solution, and diluting to certain volume; adjusting the pH value with hydrochloric acid and adding the defoaming agent; and sub-packaging and sterilizing by high pressure steam. The liquid strain culture medium solves the advantages of prior art, such as high cost due to yeast extract and peptone as nitrogen source and monosaccharide as carbon source, and complex operation process, insufficient nutrition and inadaptation for industrialized production requirement due to decoctions of potato and bran as culture medium.

Description

Industrial production liquid culture medium of jiji mushroom and its preparation method technical field The invention relates to a culture medium for edible fungi and a preparation method thereof, in particular to a liquid culture medium for industrial production of shimeji mushrooms and a preparation method thereof. Background technique Edible mushroom liquid medium can be divided into natural medium, semi-synthetic medium and synthetic medium. An excellent medium should have the advantages of fast mycelial growth, short fermentation cycle, low cost of medium, and wide sources. Like other microorganisms in the industry, setting a reasonable medium is an important link for the normal growth and metabolism of shimeji mushrooms under liquid fermentation conditions. The medium used for the liquid fermentation of Shijiji mushroom is preferably natural medium, and the nitrogen source should be mainly organic nitrogen source. At present, most of the culture media used for liqu...

AI Technical Summary

Problems solved by technology

At present, most of the culture media used for liquid culture of edible fungi are medium for laboratory experiments. Most of these culture media use yeast extract, peptone, etc. as nitrogen sources, and monosaccharides such as glucose as carbon sources. The cost is