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Preparation, storage and use of umbilical cord and placenta mesenchymal stem cell for clinical therapy

A kind of stem cell and stem cell technology, applied in the field of umbilical cord placenta stem cells, can solve the problems of umbilical cord pollution, cross-contamination of other specimens, cross-contamination of liquid phase, etc., to achieve clinical application guarantee, save storage space, and avoid pollution effects

Inactive Publication Date: 2008-11-05
TIANJIN HUANYUSNGQIAO BUSINESS INFORMATION CONSULTING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many normal delivery umbilical cords are not processed well, making them unusable for stem cell storage
[0007] 2Because some umbilical cords are contaminated by pathogens, some umbilical cords obtained during the culture process, bacteria, molds, etc. reproduce, causing pollution to other cells and other individual storage
[0008] 3 What is more serious is that at present, the method of immersion in liquid nitrogen is often used in general storage, which will increase the risk of cross-contamination between the stored cells through the liquid nitrogen liquid phase
[0009] 4 When using a plastic storage tube with a cover, the chance of cross-contamination of other specimens is also greatly increased due to leakage from the cover of the tube
[0010] The umbilical cord and placental mesenchymal stem cells can be stored in these individuals due to the carrier nature of normal delivery, and even these storages are limited to cesarean section
What's more serious is that bacteria, mold, liquid-phase cross-contamination in various items, and cross-contamination caused by leakage of stored materials from the nozzle will cause huge problems for the application of clinical treatment in the future
It also leaves hidden dangers for the stored stem cells to be polluted by pathogens, and for the cross-contamination of cells, DNA, RNA, etc. between stored stem cells of different individuals
Thereby burying potential dangers for the clinical application of these stem cells, making these stem cells lose their clinical application value

Method used

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  • Preparation, storage and use of umbilical cord and placenta mesenchymal stem cell for clinical therapy
  • Preparation, storage and use of umbilical cord and placenta mesenchymal stem cell for clinical therapy

Examples

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Effect test

Embodiment 1

[0036] Normal vaginal delivery placenta, decontamination of umbilical cord. Flush the placenta and umbilical cord with sterile saline to remove visible dirt and residual blood. Use 2% medical iodine for 30 seconds, then deiodine with 75% medical alcohol. The outer surface of the umbilical cord placenta was separated by hand about 0.5 mm. The outer surface does not touch the sterile inner tissue during removal. The stored individuals are routinely tested for tissue typing (HLA), biology, and pathogenic microorganisms. At the same time, we will establish databases for clinical umbilical cords and placental mesenchymal stem cells, and prepare data cards and barcodes that are consistent with the various types of cells stored, personal data databases, and various reports. Preparation and culture of mesenchymal stem cells.

[0037] Umbilical cord or placental tissue is processed to less than 1 mm in size using sterile manual, or automated methods. Centrifuge at 250g for 5 minut...

Embodiment 2

[0040]Normal vaginal delivery placenta, decontamination of umbilical cord. Flush the placenta and umbilical cord with sterile saline to remove visible dirt and residual blood. Use 2% medical iodine and 75% medical alcohol to treat the surface of the umbilical cord and placenta. According to the situation, about 0.5mm of the outer surface of the umbilical cord placenta was removed manually and with special instruments. The outer surface does not touch the sterile inner tissue during removal. The stored individuals are routinely tested for tissue matching (HLA), biology, and pathogenic microorganisms. At the same time, we will establish databases for clinical umbilical cords and placental mesenchymal stem cells, and prepare data cards and barcodes that are consistent with the various types of cells stored, personal data databases, and various reports. Preparation and culture of mesenchymal stem cells. Allows automated methods to process umbilical cord or placental tissue to ...

Embodiment 3

[0043] The placenta and umbilical cord were flushed with sterile saline containing penicillin and streptomycin to remove residual blood. With medical H 2 o 2 Quickly process placenta, umbilical cord surface. According to the situation, the outer surface of the umbilical cord placenta of about 0.5 mm is removed by manual separation or special equipment. The outer surface does not touch the sterile inner tissue during removal. The stored individuals are routinely tested for tissue typing (HLA), biology, and pathogenic microorganisms. At the same time, we will establish databases for clinical umbilical cords and placental mesenchymal stem cells, and prepare data cards and barcodes that are consistent with the various types of cells stored, personal data databases, and various reports.

[0044] Umbilical cord or placental tissue is processed to less than 1 mm in size using sterile manual, or automated methods. Tissue can further be processed to smaller particles using sterile...

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Abstract

The invention discloses the preparation, the storage and an application of a stem cell used for the clinical care. The establishment of the invention comprises the following steps that: 1) the decontamination treatment of a placenta and a umbilical cord is performed; a) medical iodine and alcohol or other disinfector are adopted to process the surfaces of the placenta and the umbilical cord; b) the outer surfaces of the placenta and the umbilical cord are removed by an instrument or manually; c) the loss of the bacterium and the stem cell of the placenta and the umbilical cord tissue; 2) the preparation and culture without bacteria of the mesenchyma stem cell; a) the tissue is processed manually or automatically without bacteria; b) the tissue is grinded into the smallest particles without bacteria; c) the tissue in the 2b item of the enzyme treatment is prepared; d) the mixture is washed and separated centrifugally to collect the cell; e) the cell is cultured; 3) the umbilical cord mesenchyma stem cell is stored safely; a) the cell is collected into a storage pipe or a bag in a sterile way; b) a sealing cover is added on the storage pipe; c) the double layer of the high quality plastic film is covered and each pipe is sealed by a pumping method; d) the cell is stored inside a fluid phase of a steam type liquid nitrogen storage tank, or the safe injection fluid is prepared.

Description

technical field [0001] The invention relates to an umbilical cord placental stem cell used for clinical treatment, and its preparation, storage and application. Specifically, it relates to a method different from general cell banks, but umbilical cord and placental stem cells, banks, and their preparation and storage aimed at clinical treatment. [0002] background [0003] Due to the potential clinical therapeutic effect of stem cells on various diseases, stem cells have been extensively studied in basic medical experimental methods. However, due to the characteristics of its basic research and the characteristics of the excessive way to clinical application, these researches are lacking in the preparation, storage and application of stem cells for clinical treatment. It also leaves hidden dangers for the contamination of the stored stem cells by pathogens and the cross-contamination between different stored individual stem cells. Thereby laying potential dangers for the c...

Claims

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Application Information

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IPC IPC(8): C12N5/08G06F7/00C12N5/0775
CPCA01N1/0278A01N1/02
Inventor 王福生
Owner TIANJIN HUANYUSNGQIAO BUSINESS INFORMATION CONSULTING
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