Preparation, storage and use of umbilical cord and placenta mesenchymal stem cell for clinical therapy
A kind of stem cell and stem cell technology, applied in the field of umbilical cord placenta stem cells, can solve the problems of umbilical cord pollution, cross-contamination of other specimens, cross-contamination of liquid phase, etc., to achieve clinical application guarantee, save storage space, and avoid pollution effects
Inactive Publication Date: 2008-11-05
TIANJIN HUANYUSNGQIAO BUSINESS INFORMATION CONSULTING
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The invention discloses the preparation, the storage and an application of a stem cell used for the clinical care. The establishment of the invention comprises the following steps that: 1) the decontamination treatment of a placenta and a umbilical cord is performed; a) medical iodine and alcohol or other disinfector are adopted to process the surfaces of the placenta and the umbilical cord; b) the outer surfaces of the placenta and the umbilical cord are removed by an instrument or manually; c) the loss of the bacterium and the stem cell of the placenta and the umbilical cord tissue; 2) the preparation and culture without bacteria of the mesenchyma stem cell; a) the tissue is processed manually or automatically without bacteria; b) the tissue is grinded into the smallest particles without bacteria; c) the tissue in the 2b item of the enzyme treatment is prepared; d) the mixture is washed and separated centrifugally to collect the cell; e) the cell is cultured; 3) the umbilical cord mesenchyma stem cell is stored safely; a) the cell is collected into a storage pipe or a bag in a sterile way; b) a sealing cover is added on the storage pipe; c) the double layer of the high quality plastic film is covered and each pipe is sealed by a pumping method; d) the cell is stored inside a fluid phase of a steam type liquid nitrogen storage tank, or the safe injection fluid is prepared.
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