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Method for determining saponin content of SmilaxscobinicaulisC.H.Wringh or its formulation

A technology of smilax smilax and saponin, which is applied in the field of indirect determination of sarsaparilla smilax or its preparations by colorimetry, can solve the problems of quality control, resource evaluation, extraction and separation, unfavorable modernization utilization of Chinese herbal medicines, etc. , to achieve the effect of stable and reliable method, strong reaction specificity and good reproducibility

Inactive Publication Date: 2009-01-07
NORTHWEST A & F UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The main active ingredients in Smilax chinensis or its preparations are various steroidal saponins with Laxogenin as the non-sugar part. At present, there is no literature about the determination method of saponin in Smilax chinensis or its preparations According to reports, this has brought great troubles to the quality control, resource evaluation and extraction and separation of active ingredients of Smilax nigra medicinal materials and their preparations with saponin as the main active ingredient, which is not conducive to the modern utilization of traditional Chinese medicinal materials. Therefore, It is of great significance to determine the content of sapogenin in Smilax chinensis and its preparations

Method used

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  • Method for determining saponin content of SmilaxscobinicaulisC.H.Wringh or its formulation
  • Method for determining saponin content of SmilaxscobinicaulisC.H.Wringh or its formulation
  • Method for determining saponin content of SmilaxscobinicaulisC.H.Wringh or its formulation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: Maximum Absorption Wavelength Determination

[0016] Precisely draw 1mL of Laxogenin solution into a colorimetric tube, heat in a water bath to evaporate the solvent, add 1mL of vanillin-glacial acetic acid-perchloric acid solution, heat in a water bath at 60°C for 15min, immediately cool in an ice-water bath for 10min after taking it out, and then accurately Add 5.0mL of glacial acetic acid to dilute, shake well, and scan in the range of 400-650nm with a UV-visible spectrophotometer. It is found that both the reference solution and the sample solution have a maximum absorption peak at 450nm and are relatively stable, while the blank solution has only weak absorption here. , the absorption curve is shown in the appendix figure 1 .

Embodiment 2

[0017] Embodiment 2: color reaction temperature is determined

[0018] Precisely draw 1mL of Laxogenin solution into a colorimetric tube, heat in a water bath to evaporate the solvent, add 1mL of vanillin-glacial acetic acid-perchloric acid solution, heat in a water bath at different temperatures for 15min, and immediately cool in an ice-water bath for 10min after taking it out. Accurately add 5.0 mL of glacial acetic acid to dilute, shake well, and scan with a UV-visible spectrophotometer in the range of 400-650 nm. The scanning results are shown in Table 1. It can be seen from Table 1 that when the water bath temperature is 40-60°C, λmax changes little; when the temperature is greater than 70°C, λmax changes greatly; when the temperature is 60°C, the absorbance value (A) is the largest, and the λmax changes the least, Therefore, the color reaction temperature was set at 60°C.

[0019] Table 1 Water bath temperature versus λ max and A influence

[0020] Water bath...

Embodiment 3

[0021] Embodiment 3 stability test

[0022] Accurately draw 0.5mL of the reference solution into the colorimetric tube (make a blank control at the same time), heat in a water bath to evaporate the solvent, add 1.0mL of vanillin-glacial acetic acid-perchloric acid solution, heat in a water bath at 60°C for 15min, take it out immediately Cool in an ice-water bath for 10 minutes, then accurately add 5.0 mL of glacial acetic acid to dilute, shake well, measure the absorbance value A at 450 nm with a UV-visible spectrophotometer, measure A within 0 to 100 minutes, and see the results in figure 2 .

[0023] figure 2 It shows that the absorbance value A of the chromogenic system is unstable within 0-30 minutes; the absorbance value is relatively stable within 30-70 minutes; after more than 70 minutes, A decreases obviously. Therefore, the data measured within 30-70 minutes after color development are reliable.

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Abstract

The invention discloses a method which is used for determining the content of sapogenin in smilax scobinicaulis or a preparation thereof. In the method, acidic aqueous solution is added into the smilax scobinicaulis or the preparation thereof to decompose the saponins; the soluble organic solvents of dichloroethane or chloroform, ethyl acetate and so on, which can not be dissolved in water, are used for extracting the sapogenin; under the influence of perchloric acid, the sapogenin and vanillin conduct color reaction in glacial acetic acid; the sapogenin is used as a reference substance; the colorimetry is used for determining the content of the sapogenin in the position of the wavelength of 450nm. The method can determine the content of the sapogenin in the smilax scobinicaulis or the preparation thereof, and has the advantages of strong reaction specificity, excellent reproducibility, stability, and reliability.

Description

technical field [0001] The invention relates to a method for measuring the content of saponin in Smilax chinensis or its preparation, in particular to a method for indirect determination of sapogenin in Smilax smilax or its preparation by using vanillin as a colorimetric method content method. Background technique [0002] Black-thorned smilax (Smilax scobinicaulis C.H.Wright), also known as short-stalked smilax, is a plant of the genus Smilax in the family Smilax. It grows in low mountain shrubs or valley river banks. It is mainly produced in Shaanxi and Gansu provinces, and is widely distributed in northern regions. The resources are very rich. Its rhizome is used as medicine instead of Clematis in northern my country, and it is called "wire clematis", "King Kong thorn", "King Kong vine" and so on. Decoction or soaked in wine for oral administration; grinding into powder, grinding juice for application or decocting for external use; it has the functions of eliminating rh...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 张存莉王淼邵宜添管桦邹勇毕德明唐国强
Owner NORTHWEST A & F UNIV
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