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Process for degrading cultivation waste water nitrite using immobilized nitrifying bacteria

A technology of nitrifying bacteria and nitrite, applied in the field of degrading nitrite in aquaculture wastewater, can solve the problems of energy consumption and difficulty in realization, and achieve the effects of low cost, cost reduction and high biological activity

Inactive Publication Date: 2011-04-27
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method involves a lot of equipment in the process of operation and consumes a lot of energy
This is relatively difficult to achieve under the current domestic farming conditions

Method used

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  • Process for degrading cultivation waste water nitrite using immobilized nitrifying bacteria
  • Process for degrading cultivation waste water nitrite using immobilized nitrifying bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Preparation of nitrifying bacteria concentrated solution: measure the optical density (OD) of 500ml fermented Nitrobacter victorii 600 ) is 0.765, the bacterium solution is left standstill for 8h, the bacterium will be flocculated and deposited on the bottom of the container, remove the supernatant, collect the concentrated thalline at the bottom, measure the concentrated solution volume 10ml, the thalline concentration of the concentrated solution can be obtained by the following formula:

[0033] Bacteria concentration (g / L) = 1.271×OD 600 ×V 1 / V 2 =1.271×0.765×500 / 10=48.62

[0034] Then the concentrated bacterial solution was released to 30 g / L with physiological saline to obtain the concentrated solution of nitrifying bacteria for embedding.

[0035](2) Preparation of immobilized nitrifying bacteria particles: 200ml weight percent concentration is 8% polyvinyl alcohol (PVA), 3% sodium alginate (SA) and 2% water mixture heating of silicon dioxide to fully dis...

Embodiment 2

[0040] (1) Preparation of nitrifying bacteria concentrated solution: measure the optical density (OD 600 ) is 0.780, the bacterium liquid is left standstill for 14h, the bacterium will be flocculated and deposited on the bottom of the container, remove the supernatant, collect the concentrated thalline at the bottom, measure the concentrated liquid volume 15ml, the thalline concentration of the concentrated liquid can be obtained by the following formula

[0041] Bacteria concentration (g / L) = 1.271×OD 600 ×V 1 / V 2 =1.271×0.780×500 / 15=33.05 Then release the concentrated bacterial solution to 20g / L with physiological saline to obtain the concentrated solution of nitrifying bacteria for embedding;

[0042] (2) Preparation of immobilized nitrifying bacteria granules: fully dissolve by heating the water mixture of 10% polyvinyl alcohol (PVA), 1% sodium alginate (SA) and 2% silicon dioxide with 200ml weight percent concentration 1. Mix well to make embedding solution. After coo...

Embodiment 3

[0047] (1) Preparation of nitrifying bacteria concentrated solution: measure the optical density (OD 600 ) is 0.803, the bacterium solution is left standstill for 11h, the bacterium will be flocculated and deposited on the bottom of the container, remove the supernatant, collect the concentrated thalline at the bottom, measure the concentrated solution volume 14ml, the thalline concentration of the concentrated solution can be obtained by the following formula

[0048] Bacteria concentration (g / L) = 1.271×OD 600 ×V 1 / V 2 =1.271×0.803×500 / 14=36.45

[0049] Then release the concentrated bacterial solution to 25g / L with physiological saline to obtain the concentrated solution of nitrifying bacteria for embedding;

[0050] (2) Preparation of immobilized nitrifying bacteria particles: fully dissolving by heating the water mixture of 9% polyvinyl alcohol (PVA), 2% sodium alginate (SA) and 1% silicon dioxide with 200ml weight percent concentration 1. Mix well to make embedding s...

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Abstract

The invention discloses a process for removing nitrite in aquaculture wastewater by immobilized nitrobacteria. The process comprises the preparation of nitrobacteria concentrated solution, the preparation of nitrobacteria immobilized particles, the activation of the nitrobacteria immobilized particles, the preparation of a nitrobacteria immobilized particle reaction packet and the removal of nitrite in the aquaculture wastewater by the reaction packet, wherein the preparation of the nitrobacteria immobilized particles comprises the steps that an aqueous mixture of polyvinyl alcohol, sodium alginate and silicon dioxide is heated and fully dissolved, and is evenly mixed so as to be made into embedding solution; when the embedding solution is cooled down to room temperature, embedding nitrobacteria concentrated solution is added in the embedding solution; after evenly mixed, the mixed solution is dripped into a crosslinking agent; the crosslinking agent consists of calcium chloride solution and saturated boric acid solution which are mixed according to the volume ratio of between 1 to 1 and 1 to 3; and finally, the immobilized particles are filtered out by a piece of gauze. The process has the advantages that nitrite has quick, continuous and stable degradation effect, and maintains high biological activity for a long time; moreover, the reaction packet can be used repeatedly andhas the advantages of simple manufacturing method and low cost, thereby having enormous application prospect.

Description

technical field [0001] The invention relates to a process for degrading nitrite in aquaculture wastewater, in particular to a process for treating nitrite in aquatic high-density aquaculture wastewater by using immobilized nitrifying bacteria. Background technique [0002] In recent years, due to economic and social development, people's demand for aquatic products is increasing, which prompts the rapid promotion of high-density aquaculture. High-density aquaculture often suffers from the deterioration of the water environment. Accumulation of nitrite in aquaculture water is one of the common problems. At present, people generally tend to directly add probiotics to water bodies to reduce nitrite. But this method has the disadvantages that microorganisms are easy to die and easy to lose. In order to solve this problem, some people gradually use immobilized microorganisms to solve the problem of aquatic water bodies. The immobilization of microorganisms can be divided into...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/04C02F3/34C02F1/58
Inventor 林炜铁崔华平朱雅楠罗剑飞
Owner SOUTH CHINA UNIV OF TECH
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