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Method for cultivating yeast with beer waste water

A technology of beer wastewater and yeast, which is applied in the direction of microorganism-based methods, chemical instruments and methods, food industry wastewater treatment, etc., can solve the problem of no retrieval of beer wastewater to cultivate yeast, etc., to reduce treatment costs, improve water quality, Emission reduction effect

Inactive Publication Date: 2009-01-21
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no report on the use of beer wastewater to cultivate yeast for the treatment of polluted rivers

Method used

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  • Method for cultivating yeast with beer waste water
  • Method for cultivating yeast with beer waste water
  • Method for cultivating yeast with beer waste water

Examples

Experimental program
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Effect test

Embodiment 1

[0025] A method for culturing yeast with beer wastewater, comprising the following steps:

[0026] (1) Preparation of cornstarch saccharification solution: put 80g cornstarch into 400ml distilled water and stir evenly, indirectly heat to 75°C, gelatinization reaction for 32min, cool to 45°C, add CaCl whose mass percentage concentration is 2% 2 Aqueous solution 2ml, 0.7g α-amylase, liquefy at 50°C for 8min, heat up to 100°C, inactivate the enzyme for 16min, cool to 40°C, adjust pH to 4.0, add 0.7g saccharification enzyme, saccharify at 40°C for 25h to make corn starch saccharification liquid;

[0027] (2) Select brewery wastewater: get the wastewater of beer production plant, chemical oxygen demand (CODcr) in the wastewater is 1033mg / L, five-day biochemical oxygen demand (BOD 5 ) is 914mg / L, NH 4 -N is 11mg / L, let stand for 35min, take the supernatant;

[0028] (3) Preparation of culture medium: mix 1000ml of supernatant with 8g of commercially available industrial powdered ...

Embodiment 2

[0031] A method for culturing yeast with beer wastewater, comprising the following steps:

[0032] (1) Preparation of cornstarch saccharification solution: put 70g cornstarch into 400ml distilled water and stir evenly, indirectly heat to 70°C, gelatinize reaction for 35min, cool to 50°C, add CaCl whose mass percentage concentration is 1% 2 Aqueous solution 2.5ml, 0.8g α-amylase, liquefy at 45°C for 5min, heat up to 100°C, inactivate the enzyme for 15min, cool to 35°C, adjust the pH to 3.8, add 0.8g saccharification enzyme, saccharify at 38°C for 26h to make Corn starch saccharification liquid;

[0033] (2) choose brewery waste water: get the waste water of beer production plant, CODcr in described waste water is 1421mg / L, BOD 5 1099mg / L, NH 4 -N is 21mg / L, let stand for 40min, take the supernatant;

[0034] (3) Preparation of culture medium: mix 1000ml of the supernatant with 10g of powdered corn steep liquor, stir to dissolve it, add 6ml of the cornstarch saccharification ...

Embodiment 3

[0037] A method for culturing yeast with beer wastewater, comprising the following steps:

[0038] (1) Preparation of cornstarch saccharification solution: put 90g cornstarch into 400ml distilled water and stir evenly, indirectly heat to 80°C, gelatinize for 30min, cool to 20°C, add CaCl whose mass percentage concentration is 3% 2 Aqueous solution 2ml, 0.8g α-amylase, liquefy at 46°C for 10min, heat up to 100°C, inactivate enzyme for 17min, cool to 40°C, adjust pH to 4.5, add 0.7g saccharification enzyme, saccharify at 42°C for 24h to make corn starch saccharification liquid;

[0039] (2) Select brewery wastewater: take the wastewater from the beer production plant, and adjust the CODcr of the wastewater to be 1000 mg / L, BOD 5 1300mg / L, NH 4 -N is 10mg / L, let stand for 30min, take the supernatant; (CODcr, BOD in this example 5 , NH 4 -N concentration is laboratory adjusted)

[0040](3) Preparation of culture medium: mix 1000ml of the supernatant with 7g of powdered corn s...

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Abstract

The invention discloses a method for culturing microzyme by beer waste water, comprising the following steps: (1) a step of preparing corn flour saccharification solution; (2) a step of selecting the beer waste water, in which the waste water in a beer manufacturing plant is kept stand for 30 to 40 minutes, and clear solution is selected; (3) a step of preparing a culture medium, in which 1, 000 milliliters of the clear solution is mixed with 7 to 10 grams of powder-shaped corn steep liquor and is stirred to dissolve the corn steep liquor, 4 to 6 milliliters of the corn flour saccharification solution is added to adjust the pH value between 4 and 5, the mixture is divided and put into different containers to be sterilized and to prepare the culture medium; and (4) a step of culturing microzyme, in which the microzyme solution is inoculated in the culture medium prepared in the step (3) to culture. The method takes the mixed waste water discharged from the beer manufacturing plant as a raw material to ferment the microzyme, thereby not only reducing the emission of the beer waste water but also greatly lowering the production cost of the microbial inoculum because the nutritive material of the beer waste water is effectively utilized, and realizing that the waste is treated by the waste and changed into valuable things.

Description

technical field [0001] The invention relates to a method for culturing yeast, in particular to a method for culturing yeast with brewery wastewater. Background technique [0002] Yeast is a powerful fungus that is widely used in water treatment in addition to some traditional functions such as fermented food. Studies have shown that yeast is very effective for the treatment of polluted rivers. However, since a large amount of inoculants are required for inoculation in the river, the cost of cultivating yeast by traditional methods is too high, so the treatment cost of adding yeast to the river is often higher than that of other processes. Therefore, although the method of adding yeast to treat polluted rivers has the advantages of simplicity and no secondary pollution, it is difficult to be used in practical projects. [0003] Wastewater recycling has always been widely concerned, such as: "Method for producing photosynthetic bacteria cells with molasses alcohol mash" disc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C02F3/34C12R1/645C02F103/32
Inventor 孙井梅常素云郑毅孙宝盛刘宝山
Owner TIANJIN UNIV