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Method for accelerating biotransformation of organic matter hard-to-degrade with co-immobilized amboceptor and thalli

A technology for the biotransformation of refractory organic matter, applied in the field of co-immobilization of mediators and bacteria to promote the biotransformation of refractory organic matter, can solve the problems of refractory organic matter bioreduction and transformation, secondary pollution, etc., and achieve the improvement of biological treatment efficiency, The effect of convenient operation and simple preparation process

Inactive Publication Date: 2009-02-18
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to co-immobilize bacteria and redox mediators in a reactor, solve the technical problem of secondary pollution caused by the loss of water-soluble redox mediators with the effluent, and strengthen the biological reduction transformation of refractory organic matter

Method used

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  • Method for accelerating biotransformation of organic matter hard-to-degrade with co-immobilized amboceptor and thalli
  • Method for accelerating biotransformation of organic matter hard-to-degrade with co-immobilized amboceptor and thalli
  • Method for accelerating biotransformation of organic matter hard-to-degrade with co-immobilized amboceptor and thalli

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Calcium alginate co-immobilization mediator and bacterial cells promote biological decolorization of azo dyes

[0066] [1] Enrichment and cultivation of quinone-reducing bacteria

[0067] The composition of the enrichment medium is (g.L -1 ): NH 4 Cl, 1.0; KH 2 PO 4 ,0.5; K 2 HPO 4 , 0.6; MgCl 2 ·6H 2 O, 0.2; CaCl 2 2H 2 O, 0.05; Na 2 S·9H 2 O, 0.037; pH7.0; AQDS 0.15g.L -1 , glucose 0.5g.L -1 . The inorganic salt medium is an enriched medium without glucose and AQDS.

[0068] Put 100mL of the above-mentioned enrichment medium and 10mL of activated sludge into a 135mL serum bottle, fill the serum bottle with the enrichment medium, seal it with a silica gel plug, and place it in a 30°C incubator for static culture. 2-3 days is an enrichment period. After each cycle, the bacterial solution was transferred into fresh enrichment medium. Enrichment ended after 8 cycles. The flora was transferred to enriched medium for expansion culture. Place it in an anaer...

Embodiment 2

[0081] Calcium alginate co-immobilization mediator and bacterial cells promote the biotransformation of nitroaromatics

[0082] [1] Enrichment and cultivation of quinone-reducing flora according to Example 1

[0083] [2] Select anthraquinone as the water-insoluble mediator

[0084] [3] Preparation of co-immobilization mediator and bacteria according to Example 1

[0085] [4] Co-immobilized mediator and bacteria to accelerate the biotransformation of nitroaromatics

[0086] Performed in 135mL serum bottles. In an anaerobic incubator, add co-immobilized spheroids or immobilized spheroids, glucose, nitroaromatics dissolved in acetone to serum bottles, and fill with inorganic salt medium. The final concentration of bacteria balls is 120g.L -1 , the glucose concentration is 0.5g.L -1 , the concentration of nitroaromatics is 100mg.L -1 . Use a rubber stopper to seal the mouth of the bottle, and culture it statically in an anaerobic incubator at 30°C for 3 days.

Embodiment 3

[0088] Calcium alginate co-immobilization mediator and bacterial cells promote biological dechlorination of polychlorinated organic compounds

[0089] [1] Enrichment and cultivation of quinone-reducing flora according to Example 1

[0090] [2] Select anthraquinone as the water-insoluble mediator

[0091] [3] Preparation of co-immobilization mediator and bacteria according to Example 1

[0092] [4] Co-immobilization of mediators and bacteria to promote the biological dechlorination of polychlorinated organic compounds

[0093] Performed in 135mL serum bottles. In an anaerobic incubator, add co-immobilized spheroids or immobilized spheroids, glucose, polychlorinated organics into serum bottles, and fill with inorganic salt medium. The final concentration of bacteria balls is 120g.L -1 , the glucose concentration is 0.5g.L -1 , the concentration of polychlorinated organic compounds is 50mg.L -1 . Use a rubber stopper to seal the mouth of the bottle, and culture it in an anae...

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Abstract

The invention provides a method of promoting refractory organics to be degraded by co-immobilized mediator and thalli, belonging to the environment engineering water treatment technical field. The method is characterized in that embedding method, membrane reactor method or grain sludge method are used to co-immobilize the thalli and water insoluble mediator in a biological reactor and promote the biological reductive conversion of the refractory organics in water, with the result that the whole biological treatment efficiency of refractory organic waste water is greatly improved. The invention has the beneficial effect that the co-immobilized water insoluble mediator and thalli has simple preparation technology, is convenient to be operated, solves the secondary pollution problem caused by the running out of the water soluble mediator with the water, and can be widely used in the biological treatment of the refractory organic waste water such as azo dyes, nitro arene, polychlorinated organic compounds and so on.

Description

technical field [0001] The invention belongs to the technical field of environmental engineering water treatment, and relates to a method for co-immobilizing mediators and bacteria to promote biotransformation of refractory organic matter. Background technique [0002] With the development of industry and agriculture, a large number of toxic and refractory organic substances have been produced, such as halogenated, azo-containing and nitroaromatic compounds, etc. These compounds are widely used in people's production and life and enter the natural environment through various channels, showing the characteristics of long-term residue and high toxicity. Some of these substances have teratogenic or carcinogenic effects, and can bioaccumulate in the food chain, seriously threatening human life. [0003] The treatment of wastewater containing toxic and refractory organics includes physical, chemical, biological and combinations of these methods. Because the biological method ha...

Claims

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Application Information

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IPC IPC(8): C02F3/34
Inventor 王竞苏妍彦周集体吕红金若菲
Owner DALIAN UNIV OF TECH
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