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Long period optical fiber optical grating nucleic acid sensor system and detection method for detecting mononucleotide polymorphism

A single nucleotide polymorphism and nucleic acid sensor technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of poor feasibility of discovering and screening SNPs, and the inability to determine the location and characteristics of SNPs. Effect of unstable light source, realize distributed measurement, easy to operate and move

Inactive Publication Date: 2009-02-18
NANJING UNIV OF AERONAUTICS & ASTRONAUTICS
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Problems solved by technology

Since these methods require fluorescent labels and specialized analytical instruments, the feasibility of large-scale discovery and screening of SNPs is poor, and only the region where the SNP is found, but the specific location and characteristics of the SNP cannot be determined

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  • Long period optical fiber optical grating nucleic acid sensor system and detection method for detecting mononucleotide polymorphism
  • Long period optical fiber optical grating nucleic acid sensor system and detection method for detecting mononucleotide polymorphism
  • Long period optical fiber optical grating nucleic acid sensor system and detection method for detecting mononucleotide polymorphism

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Embodiment Construction

[0022] Depend on figure 1 It can be seen that the specific composition of the long-period fiber grating nucleic acid sensor system of the present invention is that a broadband light source 1 (LG150 type broadband light source can be used) enters a tree-shaped fiber coupler 2 (such as figure 2 shown) to the input end of the tree fiber coupler 2, and then through the coupler joint 3, through the sensing fiber 4 to the terminal reflective long-period fiber grating nucleic acid sensor probe 5, and the sample to be tested The DNA molecules interact with each other to produce the coupling effect between the core and the cladding. After passing through the reflective metal film on the end face of the core in the probe 10, the reflected light is formed and enters the spectrometer 6 through the reflective end of the tree fiber coupler 2 (AQ6317 can be used type spectrometer), and then processed by the computer 7 to output the relationship curve between the reflected light intensity an...

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Abstract

The invention relates to a distribution type long period fiber grating nucleic acid sensor system and a method for detecting the polymorphism of a single nucleotide which belong to the testing field of a biosensor. The system consists of a Broad Band light source (1), a tree type fiber coupler (2),a sensing fiber (4), a computer (7), a spectrograph and a terminal reflection type long period fiber grating nucleic acid sensor probe (5) or a distribution type long period fiber grating nucleic acid sensor probe. In the method, the SNP of the DNA in a sample to be detected is detected through measuring the change of a resonance peak caused by the change of a dielectric constant in the test liquid of the sample to be detected. The distribution type long period fiber grating nucleic acid sensor system and a method for detecting the polymorphism of a single nucleotide have the characteristics of high sensitivity, the realization of distributed measurement and real-time high pass detection.

Description

technical field [0001] The invention relates to a novel optical fiber DNA sensor system combining long-period optical fiber grating refractive index sensitivity and optical fiber detection technology, which can be applied to human genome research single nucleotide polymorphism (SNP). Background technique [0002] Long-period fiber grating (hereinafter referred to as LPG) is a sensing grating based on the coupling theory of core guided mode and cladding mode. concentration. Biosensing technology based on this coupling theory has been widely used in many biochemical research fields such as the measurement of antigen content in blood, the identification of protein substances, and drug screening. [0003] With the completion of the precise sequence map of the human genome, the focus of human genome research has entered the post-genome era. One of its main contents is the establishment of deoxyribonucleic acid (deoxyribonucleic acid) represented by single nucleotide polymorphism...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 曾捷梁大开刘宏月齐名张晓丽
Owner NANJING UNIV OF AERONAUTICS & ASTRONAUTICS
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