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Composite microbial preparation for methane tank and preparation thereof

A technology of composite microbial agent and viable count, which is applied in the field of agricultural microorganisms, can solve the problems of troublesome use, limited popularization, and development constraints, and achieve the effects of simple use, increased biogas production, and good repeatability

Inactive Publication Date: 2010-06-30
INST OF SOIL & FERTILIZER SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] But at present, the development of household biogas digesters in northern my country is restricted by two aspects
The first is the difficulty of biogas production in winter
The second problem is that it is difficult to produce gas with straw as the main material
The limitations of this method are: 1. Focusing on decomposing straws cannot solve the problem of biogas production in user biogas tanks in winter; 2. The compound bacterial agent can only be used when pretreating straws, and cannot be directly put into biogas tanks to promote biogas generation. The method of use is cumbersome. limited its promotion

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Pseudomonas fluorescens, Bacillus cereus, Bacillus subtilis and Bacillus circulus were purchased from the Culture Collection Center, and conventional methods were used to treat Pseudomonas fluorescens, Bacillus cereus, Bacillus subtilis and Bacillus circulus Separately ferment and prepare 4 kinds of bacterial liquids for future use, and the number of effective viable bacteria in each fermentation stock liquid should be kept at 1 billion / ml. The specific method is as follows:

[0041] (1) Activate the above four kinds of microorganisms into their respective slant culture medium for preservation, and culture them at 28-30° C. for 24-36 hours.

[0042] (2) Connect the activated strains to their respective liquid medium, Bacillus cereus (Bacillus cereus): 5 grams of beef extract, 5 grams of peptone, 5 grams of sodium chloride, 1 gram of yeast powder, KH 2 PO 4 0.5 g, MgSO 4 0.5g, 1000ml tap water, pH 7.0-7.5. Pseudomonas Putida uses: 30 grams of glucose, 10 grams of bee...

Embodiment 2

[0046] Embodiment 2: The preparation of the fermented liquid of 4 kinds of bacteria is as embodiment 1.

[0047] 2 parts of peat, 1 part of diatomaceous earth, sterilize peat and diatomaceous earth, add to the mixer, mix and add microbial agent, 0.1 part of Bacillus subtilis fermentation stock solution in the bacterial agent, 0.08 part of Bacillus cereus fermentation stock solution , 0.1 part of Pseudomonas fluorescens fermentation stock solution, 0.05 part of Bacillus circulans fermentation stock solution, the effective number of viable bacteria in each bacterial solution is 1 billion / ml, and the microbial agent is mixed with peat and diatomaceous earth for later use . The composite microbial agent is suitable for biogas digesters with straw and manure as the main raw materials.

Embodiment 3

[0048] Embodiment 3: The preparation of the fermented liquid of 4 kinds of bacteria is as embodiment 1.

[0049] 1 part of peat, 1 part of diatomaceous earth, sterilize the peat and diatomaceous earth, add to the mixer and mix, then add the microbial agent, 0.08 part of Bacillus subtilis fermentation stock solution in the bacterial agent, 0.04 part of Bacillus cereus fermentation stock solution, 0.15 part of Pseudomonas fluorescens fermentation stock solution, 0.06 part of Bacillus circulans fermentation stock solution, the effective number of viable bacteria of each bacterial solution is 1 billion / ml respectively, and the microbial bacterial agent is mixed with peat and diatomaceous earth evenly before use. The composite bacterial agent is suitable for use in biogas digesters with straw as the main raw material.

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Abstract

The invention relates to a composite microbial inoculum used in a biogas generating pit, which consists of four kinds of microbial fermentation liquor, namely, 0.06-0.15 part of pseudomonas fluorescens solution, 0.04-0.08 part of waxen bacillus solution, 0.06-0.10 part of bacillus subtillis solution and 0.05-0.08 part of bacillus circulans solution, which are mixed in proportion. The composite microbial inoculum can promote the biogas generating pit to generate biogas normally in winter and can prepare the biogas by taking straws as main raw material.

Description

technical field [0001] The invention belongs to the technical field of agricultural microbes, and in particular relates to a composite microbial bacterial agent for promoting gas production in low-temperature conditions of household biogas pits and improving the gas production of biogas pits using straw as raw materials. Background technique [0002] With the rapid development of our country's economy, the demand for energy continues to grow, and the energy shortage is becoming more and more serious. The high amount of energy has increased the pressure on farmers. Therefore, making full use of existing resources in rural areas to promote household biogas digesters in rural areas is one of the effective measures to solve rural energy problems and reduce the burden on farmers. It is estimated that a mouthful of 8-10m of normal gas production per year 3 Biogas digester, the annual biogas production is about 350-400m 3 , can replace about 800kg of coal or about 1000kg of firew...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C12N1/20C12P39/00C12P5/02C12R1/39C12R1/085C12R1/125C12R1/09
CPCY02E50/343Y02E50/30
Inventor 王梅刘兆辉江丽华刘英张文君林海涛郑福丽宋效宗王艳芹袁长波
Owner INST OF SOIL & FERTILIZER SHANDONG ACAD OF AGRI SCI