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Purification method of super tylosin

A technology of super tylosin and purification method, which can be applied in microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., and can solve the problems of high cost, easy environmental pollution, and high operation toxicity.

Active Publication Date: 2009-03-11
CHINA ANIMAL HUSBANDRY IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above method uses an organic solvent as a solvent, the operation is highly toxic, easy to pollute the environment, and the cost is high. A safer and more environmentally friendly purification method should be found in modern industrial production applications

Method used

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  • Purification method of super tylosin

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] After about 170 hours of fermentation and cultivation of super tylosin transforming bacteria in the fermenter, 50 tons of super tylosin fermentation liquid was obtained, and diluted with water to 70 tons, and the temperature of the fermentation liquid was lowered to 20°C; Add 6mol / L hydrochloric acid solution, adjust the pH of the fermentation broth to 2, and stir for 3 hours. Sampling for HPLC detection showed that the titer was 4572u / ml, and the dissolution rate of super tylosin was 96%. Plate and frame filtration, and the filtrate enters the crystallization tank. Raise the temperature of the primary acid-soluble filtrate to 40°C, add 4mol / L potassium hydroxide solution, slowly adjust the pH value to 7.5, and let it stand for 4 hours to produce super tylosin crystals. The serum titer is 82u / ml. The primary crystals were dissolved in water, the temperature of the solution was lowered to 20°C, and 6 mol / L hydrochloric acid solution was added to dissolve it all, and th...

Embodiment 2

[0022] After about 170 hours of fermentation and cultivation of the super tylosin transformed bacteria in the fermenter, 50 tons of super tylosin fermentation broth was obtained, diluted with water to 70 tons, and the temperature of the fermentation broth was lowered to 25°C; 4mol / L sulfuric acid solution, adjust the pH of the fermentation broth to 3.8, and stir for 1 hour. Sampling for HPLC detection showed that the titer was 4821u / ml, and the dissolution rate of super tylosin was 97%. Plate and frame filtration, and the filtrate enters the crystallization tank. Raise the temperature of the primary acid-soluble filtrate to 40°C, add 4mol / L sodium carbonate solution, slowly adjust the pH value to 8, and let it stand for 8 hours to produce super tylosin crystals, obtain the primary crystals by plate and frame filtration, and detect the supernatant The titer of the solution is 80u / ml. The primary crystals were dissolved in water, the temperature of the solution was lowered to ...

Embodiment 3

[0024] After about 170 hours of fermentation and cultivation of the super tylosin transformed bacteria in the fermenter, 50 tons of super tylosin fermentation liquid was obtained, and diluted to 70 tons with water, and the temperature of the fermentation liquid was lowered to 30°C; Add 8mol / L tartaric acid solution, adjust the pH of the fermentation broth to 5.5, and stir for 2 hours. Sampling for HPLC detection showed that the titer was 4427u / ml, and the dissolution rate of super tylosin was 95%. Plate and frame filtration, and the filtrate enters the crystallization tank. Raise the temperature of the primary acid-soluble filtrate to 60°C, add 6mol / L sodium bicarbonate solution, slowly adjust the pH value to 10, and let it stand for 6 hours to produce super tylosin crystals, and obtain the primary crystals by plate and frame filtration. The serum titer was 79u / ml. The primary crystals were dissolved in water, the temperature of the solution was lowered to 35°C, and 8 mol / L ...

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Abstract

The invention relates to a method for extracting super-tylosin from fermentation products of microorganism. The method adopts the secondary crystallization method, abandons the organic solvent extraction method in the prior art, can obtain products the purity of which is equivalent to that of extract of the solvent extraction method while simultaneously reducing the environmental pollution, simplifying the purification step and reducing the production cost, and is more suitable for industrial production. The products prepared by the method can be taken as bulk drugs of super-tylosin tartrate.

Description

technical field [0001] The invention relates to a method for purifying super tylosin, in particular to a method for obtaining high-purity super tylosin while obtaining high recovery rate by using acid extraction and alkali precipitation for secondary crystallization. method. Background technique [0002] Super Tylosin, the chemical name is 3-O-acetyl-4″-O-isovaleryl Tylosin (as shown in the picture). Super Tylosin is weakly alkaline in aqueous solution, and the solution is alkaline When the solution is acidic, it will crystallize out in the form of precipitation. When the solution is acidic, super tylosin forms corresponding salts with most acids and is easily soluble in water. [0003] Tylosin has been used as a special veterinary drug and feed additive in my country for many years, but due to its drug resistance, the European Union has ordered to ban the use of tylosin phosphate as a feed additive. Super Tylosin is a brand-new macrolide antibiotic obtained by biotransfor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/62C07H1/06C12R1/465
Inventor 余志强郑应华齐鹏郭丽清王华丽
Owner CHINA ANIMAL HUSBANDRY IND
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