Bacillus prodigiosus and prodigiosin producted thereby

A technology of Serratia marcescens and prodigiosin, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc., can solve problems such as systematic research that has not been reported, and achieve good research prospects, high safety, and thermal stability strong effect

Inactive Publication Date: 2009-03-25
XINJIANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There have been many domestic literature reports on the research on the properties of prodigiosin, but these prodigiosin-producing bacteria are mostly isolated from seawater or soil, but there is no systematic study on the prodigiosin-producing endophytic fungi. to report

Method used

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  • Bacillus prodigiosus and prodigiosin producted thereby
  • Bacillus prodigiosus and prodigiosin producted thereby
  • Bacillus prodigiosus and prodigiosin producted thereby

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: Screening of prodigiosin-producing strains

[0044] The present invention isolates the endophytic bacteria of Radix Paeoniae Alba for the first time, and screens the isolated 11 endophytic bacteria of Radix Paeoniae Alba, to obtain prodigiosin-producing strains. The specific steps are as follows:

[0045] Weigh 2 groups of root peony samples, each group has two parts of 7g each, first seal both ends of the material with a parafilm (because the root peony is a hollow plant) and then rinse with water for 3 minutes. Rinse 4 times with sterile water in the ultra-clean bench by crushing method, treat with 1% mercury liter for 13s, rinse 4 times with sterile water, use more than 100ml of sterile water in a 250ml triangular flask each time, and rinse under constant shaking 5min, rinse with sodium hypochlorite for 13 minutes, then rinse with sterile water for 5 times, finally add 6ml of sterile distilled water to grind, filter through three layers of sterile gauze, c...

Embodiment 2

[0047] Embodiment two, the identification of prodigiosin producing strain

[0048] 1. Observation of morphology and culture characteristics

[0049] The strain's individual morphological characteristics, colony morphological characteristics, motility, and physiological and biochemical characteristics were observed and measured with reference to "Bergey's Bacteria Identification Manual" and "Common Bacteria Identification Manual".

[0050] 1.1 Morphological structure observation

[0051] After the isolated bacteria were cultured on LB medium at 28°C for 1-3 days, the colony morphology was observed: needles, round, irregular, filamentous and branched; the edges of the colonies were neat, Corrugated, cracked, filamentous and curly; the height of the colony is flat, raised, raised, raised or sunken in the center, and the spore staining, Gram staining and bacterial morphology are observed, respectively according to the colony shape and color , cell morphology, presence or absence...

Embodiment 3

[0085] Example 3, Extraction of bacterial strain red pigment and identification of its structure

[0086] 1. Pigment extraction

[0087] Extraction takes the following two methods:

[0088] (1) Put an equal amount of absolute ethanol into the fermentation broth, vibrate on a shaker for 20 minutes, let it stand for 10 minutes, and centrifuge at 4000r / min for 10 minutes to remove impurities, and obtain a red supernatant, and continue to extract the residue 3 times repeatedly with absolute ethanol . The collected red supernatants were mixed and concentrated by a rotary evaporator to obtain a red viscous crude product.

[0089] (2) After culturing on MEA solid medium for 2 days, centrifuge at 4000r / min for 5min, add absolute ethanol to the precipitate to dissolve the pigment, centrifuge at 4000r / min for 5min, take the supernatant and concentrate it with a rotary evaporator to make it drying. Dissolve it with ethyl acetate, extract it twice with saturated NaCl solution, and dry...

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Abstract

The invention discloses a viscid Serratia marcescens with CGMCC.No.2593 and prodigiosin produced. The growth temperature of the strain is 28 DEG C to 38 DEG C, the pH value is 5 to 9, LB liquid medium is used for fermentation, 200mL of culture medium is filled in a 500mL triangular flask, sterilization is carried out under 1125 mutiplied by 121Pa for 20min, after being shaken for culture at temperature of 37 DEG C for 5 days, the culture medium is added with absolute ethyl alcohol and is shaken on a shaking bed for 20min, and then is placed statically for 10min, impurity removing is carried out with the centrifuge of 4000r / min for 10min, and through the extraction of absolute ethyl alcohol, prodigiosin Serratia marcescens pigment is produced with the output of 7.85g / L, the maximum absorption peak value m of the prodigiosin being 533.8nm and good heat stability. The prodigiosin obtained can be used as antineoplastic medicine, has important application prospect in the fields of foods, cosmetics and the like.

Description

field of invention [0001] The invention relates to the field of microbial fermentation and its product enzymes. Specifically, the present invention relates to an endophyte capable of producing prodigiosin and the technical field of producing prodigiosin. Background technique [0002] Pigments can be divided into natural pigments and synthetic pigments, which are widely used in food and cosmetic production. With the continuous deepening of medical toxicology and biological research, it has been found that most of the allowed synthetic pigments have different degrees of harm to the human body, especially carcinogenic, teratogenic, and mutagenic, which have attracted people's attention. For this reason, natural pigments are gradually replacing synthetic pigments. Because the growth and reproduction of animal and plant materials are affected by factors such as season, climate, and place of origin, and the raw materials are insufficient, the pigments extracted from them are exp...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P17/16C07D207/44C12R1/43
Inventor 阿依努尔·阿不都热合曼古丽祖热·佐努尼吾甫尔·米吉提
Owner XINJIANG UNIVERSITY
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