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Capparis spinosa extract as well as preparation method and application thereof

A technology of extracts and capers, applied in the field of natural medicinal chemistry, can solve the problems of insufficient research on chemical components

Inactive Publication Date: 2009-04-15
SHANGHAI UNIV OF T C M +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, patents 200610118369.8 and 200310113957.9 did not go deep enough on chemical components. The present invention has carried out in-depth research on the components of three kinds of extracts prepared for treating rheumatoid arthritis, rheumatoid arthritis, gout and frozen shoulder. The research has a clear understanding of the basis of its active ingredients, and found 5 new chemical ingredients

Method used

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  • Capparis spinosa extract as well as preparation method and application thereof
  • Capparis spinosa extract as well as preparation method and application thereof
  • Capparis spinosa extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: Get the dried caper fruit, crush it, weigh 11.5kg, heat and reflux extraction with 95% ethanol for 3 times, each time for 3 hours, the first 7 times the amount of 95% ethanol, and the latter two times are 5 times the amount solvent. Combine the reflux liquid, recover under reduced pressure (temperature 50-80°C, pressure 0.08-0.1MPa) to thick extract (specific gravity 1.1-1.4), freeze-dry to obtain crude extract dry extract (2.40kg).

[0041] The crude extract is dispersed and dissolved in water (1-4 times), extracted 2-3 times with petroleum ether (1-4 times), concentrated under reduced pressure (temperature 30-60°C, pressure 0.08-0.1MPa), freeze-dried to obtain petroleum Ether extract (100 g). Then extract with ethyl acetate (1-4 times), concentrate under reduced pressure (temperature 30-60°C, pressure 0.08-0.1MPa), and freeze-dry to prepare ethyl acetate extract 2 (60g) and water-soluble active fraction extract 3 (1.81kg).

Embodiment 2

[0042] Example 2: Get the dried caper fruit, crush it, weigh 11.5kg, heat and reflux extract with 85% ethanol for 3 times, each time for 3 hours, the first 7 times the amount of 85% ethanol, and the latter two times are 5 times the amount solvent. Combine the reflux liquid, recover under reduced pressure (temperature 50-80°C, pressure 0.08-0.1MPa) to concentrated extract (specific gravity 1.1-1.4), freeze-dry to obtain crude extract dry extract (2.45kg).

[0043] The crude extract is dispersed and dissolved in water (1-4 times), extracted 2-3 times with petroleum ether (1-4 times), concentrated under reduced pressure (temperature 30-60°C, pressure 0.08-0.1MPa), freeze-dried to obtain petroleum Ether extract (103g). Then extract with ethyl acetate (1-4 times), concentrate under reduced pressure (temperature 30-60°C, pressure 0.08-0.1MPa) and freeze-dry to make ethyl acetate extract (64g) and water-soluble Sexually active site extract (1.9kg).

[0044] The above-mentioned cru...

Embodiment 3

[0045] Example 3: Get the dried caper fruit, crush it, weigh 11.5kg, heat and reflux extract with 80% ethanol for 3 times, each 3h, the first 7 times the amount of 80% ethanol, and the latter two times are 5 times the amount solvent. Combine the reflux liquid, recover under reduced pressure (temperature 50-80°C, pressure 0.08-0.1MPa) to thick extract (specific gravity 1.1-1.4), freeze-dry to obtain crude extract dry extract (2.5kg).

[0046] The crude extract is dispersed and dissolved in water (1-4 times), extracted 2-3 times with petroleum ether (1-4 times), concentrated under reduced pressure (temperature 30-60°C, pressure 0.08-0.1MPa), freeze-dried to obtain petroleum Ether extract (106 g). Then extract with ethyl acetate (1-4 times), concentrate under reduced pressure (temperature 30-60°C, pressure 0.08-0.1MPa) and freeze-dry to make ethyl acetate extract (66g) and water-soluble Sexually active site extract (1.87kg). The above-mentioned crude extract, ethyl acetate ext...

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Abstract

The invention discloses caper extract and a preparation method thereof. The method comprises the following steps: medicinal materials are crushed, decocted in water or subjected to heating reflux extraction by utilization of ethanol with different concentrations, and added with the ethanol for impurity precipitation after concentration of extract; supernatant or more than 80 percent ethanol extract is subjected to concentration at reduced pressure; extract 1 is obtained after recovery of the ethanol, dried and dissolved in water, subjected to deesterification by utilization of petroleum ether, and extracted by ethyl acetate; an extracted position and a water-soluble position of the ethyl acetate are subjected to vacuum concentration and drying respectively and prepared into extract 2 and extract 3 respectively; the extract 1 contains 30 compounds including 5 novel compounds such as caper alkali A, and so on; the extract 2 contains 18 compounds such as a para hydroxybenzoic acid, and so on ; and the extract 3 contains 18 compounds including 5 novel compounds such as the caper alkali A, and so on. As proved by pharmacodynamic experiments, the extract 1, the extract 2 and the extract 3 have analgesic and anti-inflammatory activity, and can be used for preparing medicines for treating rheumatic arthritis, rheumatoid inflammation or scapulohumeral periarthritis.

Description

technical field [0001] The invention relates to natural medicinal chemistry, in particular to capers extract, its preparation method and its application in the preparation of medicines for treating rheumatoid arthritis, rheumatoid inflammation and frozen shoulder. Background technique [0002] Capparis spinosa L., also known as mouse melon, wild watermelon, mallet fruit vine, is called "Polik fruit" or "chuck" in Uighur, and is a vine of the genus Capparidaceae (Capparidaceae). It is a drought-resistant plant with great cultivation value in desert, semi-desert, arid and semi-arid areas. The plant is mainly distributed in Europe, North America, Oceania, and western Asia, and it is distributed in Xinjiang, Gansu, Inner Mongolia, Tibet and other provinces and regions in my country. Only one genus and one species are produced in China's sandy areas, and they are distributed in various sandy areas in Xinjiang, with the most deserts in the Turpan Basin. Xinjiang Uyghur folks oft...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61K31/7068A61P19/02A61P29/00A61K131/00
Inventor 王长虹王峥涛杨涛程雪梅周吉燕
Owner SHANGHAI UNIV OF T C M
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