Formula and preparation of biocatalyst for modification and improvement of petroleum fuel oil

Abstract

The invention relates to a formulation for a biocatalyst for modifying and improving petroleum fuel oil and a production method thereof. The formulation comprises the main components: 1, vegetable fat; 2, methanol; 3, candida rugosa lipase; 4, candida lipase; 5, horse radish peroxidase; 6, hydrogen peroxidase; 7, polyethylene glycol 1,000 to 5,000 (PEG for short); and 8, single methoxy polyethylene glycol 5,000 to 10,000 (MPEG for short). The production method comprises the following production processes and usage amount of raw materials is as follows, according to 1,000 liters of biocatalyst product produced once for modifying and improving the petroleum fuel oil: 1, the first step: using 'biological generator' equipment to produce 'fatty acid methyl ester or ethyl ester'; 2, the second step: taking 10 to 12 grams of hydrogen peroxidase powder and 5 grams of single methoxy polyethylene glycol 5,000 powder, adding 10 milliliters of pure distilled water into the mixture for mixing and preparing; and keeping stand the mixture for 30 minutes; 3, the third step: taking 12 to 15 grams of horse radish peroxidase powder and 8 grams of the single methoxy polyethylene glycol 5,000 powder, adding 12 milliliters of pure distilled water into the mixture for mixing; and keeping stand the mixture for 30 minutes; 4, the fourth step: mixing hydrogen peroxidase preparation and horseradish peroxidase preparation in turn in an organic solution of fatty acid monoester, and keeping stand the mixture for 60 minutes; and 5, packaging the finished product by 200 liters per bucket for standby.

Description

technical field [0001] The invention relates to a formulation of a biocatalyst used for the modification and improvement of petroleum fuel oil and a preparation method thereof. Background technique [0002] At present, there are various kinds of additive products used for fuel oil modification and improvement, and it is difficult to distinguish between good and bad; however, in general, they can be divided into three categories, one is inorganic chemical synthesis agent, and the other is nuclear magnetic resonance catalytic cracking The three types of physical action additives are bio-organic catalysts; there are few bio-organic additives on the market, and the difference from other types of products is that the proportion of bio-catalysts mixed with fuel oil is small but effective, and other types of products need to be mixed Between 1 / 1000 and 1 / 2500; biocatalysts rely on biological enzymes as catalysts, and other products rely on heavy metals such as manganese, barium, an...

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