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Sensing element based on titanic oxide embedding technology and manufacturing method thereof

A technology of titanium dioxide and sensing elements, which is applied in the field of sensors for biological system detection, can solve the problems of low sensitivity, easy inactivation of biomolecules, and long film forming time, etc., and achieve a wide range of applications

Inactive Publication Date: 2009-04-22
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The sensitive film formed in this way is stable and orderly, but the film formation time is long, the sensitivity is low, and the immobilized biomolecules are easily inactivated

Method used

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  • Sensing element based on titanic oxide embedding technology and manufacturing method thereof
  • Sensing element based on titanic oxide embedding technology and manufacturing method thereof
  • Sensing element based on titanic oxide embedding technology and manufacturing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 The sensor element structure used in the surface plasmon resonance sensor of the present invention and the comparison with the sensor element structure of the background technology.

[0029] figure 1 It is a structural schematic diagram of the sensing element in the background technology. figure 1 Among them, 1 is a glass prism, 2 is a metal film, 3 is a carboxy-terminally acylated 3-mercaptopropionic acid (MPA) film, and 4 is a rabbit anti-human IgG film. ( ) is the antibody, ( ) is an antigen.

[0030] Figure 5 It is a schematic diagram of the sensing element based on the titanium dioxide embedding technology of the present invention. Figure 5 Among them, 1 is a glass prism, 2 is a metal film, 5 is the first layer of titanium dioxide film, 6 is the second layer of titanium dioxide film (embedded gold nanoparticles), 7 is the third layer of titanium dioxide film (embedded coupled with rabbit anti-human IgG gold nanoparticles). ( )Antibody,( ...

Embodiment 2

[0032] Example 2 Fabrication of the sensing element of the present invention.

[0033] The gold nanoparticles used in the experiment were prepared in the following manner: all glass instruments used were soaked in aqua regia for 24 hours and washed with double distilled water. All solutions used need to be filtered through a 0.22 μm semi-permeable membrane before use. Under constant stirring, heat 100mL of 0.01% chloroauric acid to boiling, then add 4mL of 1% sodium citrate, and continue stirring and heating for 10 minutes. During this process, the solution turns from colorless to wine red. The preparation of the gold nanoparticles can be completed when the measured ultraviolet absorption peak of the solution is at 519.7 nm.

[0034] The gold nanoparticles are coupled with the rabbit anti-human immunoglobulin, and the rabbit anti-human immunoglobulin is added to the prepared gold nanoparticles under vigorous stirring and incubated at room temperature; centrifuged; and phospha...

Embodiment 3

[0038] Example 3 Antigen detection (1).

[0039] Fix the prism, inject double-distilled water and PBS into the flow cell, and rinse until the SPR resonance wavelength is stable. Inject different concentrations of human IgG into the flow cell sequentially, and record the change of SPR resonance wavelength with time. Due to the specific combination of rabbit anti-human IgG and human IgG, the SPR resonance wavelength shifts to the long-wave direction, and different concentrations of human IgG correspond to The resonance wavelength shift is also different. Each concentration of human IgG can react with rabbit anti-human IgG to reach equilibrium within 30 minutes. After each sample was monitored, PBS buffer was injected to rinse the flow cell. After the measurement, the sensing element needs to be stored in PBS and kept at 4°C.

[0040] figure 2 The relationship curves of human IgG concentration and resonance wavelength shift corresponding to the two sensitive membranes are gi...

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PUM

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Abstract

The invention discloses a sensing element based on an embedding technique of titanium dioxide and a preparation method thereof, which pertain to the technical field of a sensor used for detecting biological system. The sensing element is composed of a metal film (2) which is plated on the bottom surface of a glass prism (1), a titanium dioxide sol-gel film (5) on which a first layer is blank, a titanium dioxide sol-gel film (6) on which gold nano-particles are embedded in a second layer and a titanium dioxide sol-gel film (7) on which antibodies and gold nano-particles are embedded in a third layer. The processes of preparing the sensing element comprise metal film preparation, gold nano-particles and antibodies coupling and sensitive membrane preparation. The preparation method of the invention has the advantages of simpleness, high sensitivity, good stability and wide application range and can be used for monitoring all antibody antigen as well as measuring protein and medicine.

Description

technical field [0001] The invention belongs to the technical field of sensors used for biological system detection, and in particular relates to a sensing element embedded in titanium dioxide sol-gel and a manufacturing method thereof for a surface plasmon resonance sensor. Background technique [0002] Surface Plasmon Resonance (SPR) is a physical optical phenomenon. The evanescent wave of total internal reflection of light at the two-phase interface is used to induce free electrons on the metal surface to generate surface plasmons. At a certain wavelength or incident angle, the frequency of the surface plasmon and the evanescent wave are equal, and the two will resonate, the incident light is absorbed, and the energy of the reflected light drops sharply, so that a resonance peak appears on the reflection spectrum. When the refractive index of the medium on the surface of the metal film is different, the position of the resonance peak will be different. Since Liedberg et...

Claims

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Application Information

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IPC IPC(8): G01N21/55G01N21/41G01N21/552
Inventor 宋大千张寒琦曹彦波王健王丽英孙颖朱晓楠毕宁
Owner JILIN UNIV
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