Gingeli antioxidation polypeptide composition and method for producing the same

A technology for antioxidant peptides and peptide compositions, which is applied in the preparation methods of peptides, chemical instruments and methods, peptides, etc., and can solve the problems of no patent reports in process research.

Inactive Publication Date: 2009-05-06
JIANGSU UNIV
View PDF5 Cites 16 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no patent report on the process research on the pre

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Gingeli antioxidation polypeptide composition and method for producing the same
  • Gingeli antioxidation polypeptide composition and method for producing the same
  • Gingeli antioxidation polypeptide composition and method for producing the same

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0019] Example 1:

[0020] (1) Protein extraction from sesame meal

[0021] The sesame meal was pulverized with a pulverizer and dried in an oven at 105°C for 1 h. Weigh 500 g, according to the volume-to-mass ratio of n-hexane and sesame meal 3:1, degreased by stirring in a water bath at 45°C for 3 h, and centrifuged at 4000 r / min for 15 min. The supernatant was evaporated by rotary evaporation, and n-hexane was recovered, and the residue was extracted sesame oil. The centrifugal sediment was repeated twice, and air-dried to obtain a defatted sesame meal with a residual oil rate of less than 1%.

[0022] Take 500g of defatted sesame cake and dissolve it in NaHCO according to the material-liquid ratio of 1:20 3 -In NaOH buffer solution, adjust the pH value to 12.0, extract at 80°C for 1.5 hours, centrifuge at 4000 r / min for 15 minutes, repeat the extraction of the precipitate twice, combine the supernatants, adjust the pH to 5.0, concentrate, and then adjust the pH of the con...

Example Embodiment

[0041] Example 2:

[0042] (1) Extraction of protein from sesame meal is the same as in Example 1.

[0043] (2) Preparation of sesame antioxidant polypeptide:

[0044] Take 100 g of sesame protein obtained in step (1), dissolve it in 1000 mL of distilled water, adjust the pH to 8.0 with 1 mol / L NaOH solution, and preheat at 65° C. for 30 min. Add the same preheated alkaline protease, the dosage is 13.5:1 according to the ratio of substrate to enzyme, and adjust the pH to 8.0 with 2mol / L NaOH, keep the pH and temperature of the solution constant, stir the enzymolysis for 6.6h, after the reaction is completed , 85 ℃ water bath to inactivate the enzyme for 10min, adjust the pH to 5.0, centrifuge at 4500r / min for 10min, take the supernatant and discard the precipitate.

[0045] The enzymatic hydrolyzed solution was spray-dried, and the drying conditions were as follows: the air inlet was 185±5°C, the air outlet was 85±5°C, and it should be collected quickly after drying to obtai...

Example Embodiment

[0046] Example 3:

[0047] (1) Extraction of protein from sesame meal is the same as in Example 1.

[0048] (2) Preparation of sesame antioxidant polypeptide:

[0049] 100 g of sesame protein obtained in step (1) was taken, dissolved in 1000 mL of distilled water, adjusted to pH 9.0 with 1 mol / L NaOH solution, and preheated at 55° C. for 30 min. Add the same preheated alkaline protease, the dosage is 15.74:1 according to the ratio of substrate to enzyme, and adjust the pH to 9.0 with 2mol / L NaOH, keep the pH and temperature of the solution constant, and stir the enzymolysis for 7.45h. After the reaction, The enzyme was inactivated in a water bath at 90 °C for 10 min, the pH was adjusted to 5.5, 4500 r / min, centrifuged for 10 min, and the supernatant was discarded.

[0050] The enzymatic hydrolysate was spray-dried under the following drying conditions: inlet air 185±5°C, outlet air 85±5°C, and should be collected quickly after drying to obtain 92.5 g of sesame antioxidant po...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a producing technology for extracting sesame isolated protein from sesame cake and preparing sesame anti-oxidation polypeptide. The producing technology adopts alkali extraction and acid precipitation to extract the sesame protein, and adopts an enzymolysis technique to prepare the sesame anti-oxidation polypeptide; and the production process comprises degreasing and drying of the sesame cake, and extracting, condensing and drying, enzymolysis and spray drying of the protein. The technology is simple, has various product forms, and is suitable for industrialized production. The product can be directly eaten, and can be used as a food nutrient enhancer to improve the content of the protein, for example, the product is used for vegetable protein beverages, hams, microbe culture media, and the like, and can also be used as a food antioxidant to prolong guarantee period of food, such as refrigerated storage of meat emulsion, coat of fried food, and the like.

Description

technical field [0001] The invention relates to products and processing methods processed with sesame cakes as raw materials, in particular to an antioxidant polypeptide prepared from sesame cakes and a production process, in particular to an enzymatic preparation of sesame antioxidant polypeptides and a production process. technical background [0002] Sesame cake, a by-product of sesame oil production, is rich in protein, up to 38-50%, but due to the high temperature treatment in the oil production process, most of the protein has been denatured, and its nutritional value is low. Studies have shown that after enzymatic hydrolysis of sesame protein, not only the functional properties of the protein are greatly improved, but also have certain physiological activities, such as lowering blood pressure, inhibiting bacteria, and anti-tumor. [0003] There are many research reports on sesame cake, such as: "Research on Improving the Palatability of Sesame Dregs with Biotechnology...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P21/06C07K1/30A23L1/305A23L1/28A23L1/36A23J3/14A23L33/185
Inventor 董英徐斌敬璞崔恒林邵元龙杨杰
Owner JIANGSU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap