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Gingeli antioxidation polypeptide composition and method for producing the same

A technology for antioxidant peptides and peptide compositions, which is applied in the preparation methods of peptides, chemical instruments and methods, peptides, etc., and can solve the problems of no patent reports in process research.

Inactive Publication Date: 2009-05-06
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no patent report on the process research on the preparation of antioxidant polypeptide products from sesame cake protein

Method used

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  • Gingeli antioxidation polypeptide composition and method for producing the same
  • Gingeli antioxidation polypeptide composition and method for producing the same
  • Gingeli antioxidation polypeptide composition and method for producing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Sesame cake protein extraction

[0021] The sesame cake was pulverized with a pulverizer, and dried in an oven at 105°C for 1 h. Weigh 500g, according to the volume-to-mass ratio of n-hexane and sesame cake 3:1, stir and degrease in a water bath at 45°C for 3h, and centrifuge at 4000r / min for 15min. The supernatant was rotatably evaporated to recover n-hexane, the residue was extracted sesame oil, and the centrifuged sediment was repeated twice, and naturally dried to obtain defatted sesame cake with a residual oil rate of <1%.

[0022] Take 500g of defatted sesame cake, and dissolve it in NaHCO according to the ratio of material to liquid at 1:20 3 -In NaOH buffer solution, adjust the pH to 12.0, extract at 80°C for 1.5h, centrifuge at 4000r / min for 15min, repeat the extraction twice for the precipitate, combine the supernatant, adjust the pH to 5.0, concentrate, and then adjust the pH of the concentrate to 3.0 , to obtain acid heavy protein. The acid-precipitat...

Embodiment 2

[0042] (1) Sesame cake protein extraction is the same as in Example 1.

[0043] (2) Preparation of sesame antioxidant polypeptide:

[0044] Take 100 g of sesame protein obtained in step (1), dissolve it in 1000 mL of distilled water, adjust the pH to 8.0 with 1 mol / L NaOH solution, and preheat at 65° C. for 30 min. Add the same preheated alkaline protease, the dosage is 13.5:1 according to the ratio of substrate to enzyme, and adjust the pH to 8.0 with 2mol / L NaOH, keep the pH and temperature of the solution constant, stir for 6.6h, and after the reaction , inactivate the enzyme in a water bath at 85°C for 10 minutes, adjust the pH to 5.0, centrifuge at 4500 r / min for 10 minutes, take the supernatant and discard the precipitate.

[0045] Spray-dry the enzymatic hydrolysis solution under the following drying conditions: air inlet 185±5°C, outlet air 85±5°C, and should be collected quickly after drying to obtain 90.32g of sesame antioxidant polypeptide composition product. The...

Embodiment 3

[0047] (1) Sesame cake protein extraction is the same as in Example 1.

[0048] (2) Preparation of sesame antioxidant polypeptide:

[0049] Take 100 g of sesame protein obtained in step (1), dissolve it in 1000 mL of distilled water, adjust the pH to 9.0 with 1 mol / L NaOH solution, and preheat at 55 ° C for 30 min. Add the same preheated alkaline protease, the dosage is 15.74:1 according to the ratio of substrate to enzyme, and adjust the pH to 9.0 with 2mol / L NaOH, keep the pH and temperature of the solution constant, stir for 7.45h, and after the reaction, Inactivate the enzyme in a water bath at 90°C for 10 minutes, adjust the pH to 5.5, centrifuge at 4500 r / min for 10 minutes, take the supernatant and discard the precipitate.

[0050] Spray-dry the enzymatic hydrolysis solution under the following drying conditions: air inlet 185±5°C, outlet air 85±5°C, and should be collected quickly after drying to obtain 92.5g of sesame antioxidant polypeptide composition product. The...

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Abstract

The invention relates to a producing technology for extracting sesame isolated protein from sesame cake and preparing sesame anti-oxidation polypeptide. The producing technology adopts alkali extraction and acid precipitation to extract the sesame protein, and adopts an enzymolysis technique to prepare the sesame anti-oxidation polypeptide; and the production process comprises degreasing and drying of the sesame cake, and extracting, condensing and drying, enzymolysis and spray drying of the protein. The technology is simple, has various product forms, and is suitable for industrialized production. The product can be directly eaten, and can be used as a food nutrient enhancer to improve the content of the protein, for example, the product is used for vegetable protein beverages, hams, microbe culture media, and the like, and can also be used as a food antioxidant to prolong guarantee period of food, such as refrigerated storage of meat emulsion, coat of fried food, and the like.

Description

technical field [0001] The invention relates to products and processing methods processed with sesame cakes as raw materials, in particular to an antioxidant polypeptide prepared from sesame cakes and a production process, in particular to an enzymatic preparation of sesame antioxidant polypeptides and a production process. technical background [0002] Sesame cake, a by-product of sesame oil production, is rich in protein, up to 38-50%, but due to the high temperature treatment in the oil production process, most of the protein has been denatured, and its nutritional value is low. Studies have shown that after enzymatic hydrolysis of sesame protein, not only the functional properties of the protein are greatly improved, but also have certain physiological activities, such as lowering blood pressure, inhibiting bacteria, and anti-tumor. [0003] There are many research reports on sesame cake, such as: "Research on Improving the Palatability of Sesame Dregs with Biotechnology...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/30A23L1/305A23L1/28A23L1/36A23J3/14A23L33/185
Inventor 董英徐斌敬璞崔恒林邵元龙杨杰
Owner JIANGSU UNIV
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