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Inspection method of streptococcus in cosmetics

An inspection method and technology of cosmetics, which is applied in the field of cosmetic microbial inspection and bacteria inspection in cosmetics, and can solve problems such as dermatitis, folliculitis, boils, and no detection method

Inactive Publication Date: 2009-05-06
新疆出入境检验检疫局检验检疫技术中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, streptococcus can cause infectious diseases, such as streptococcus in cosmetics, it is easy to cause dermatitis, folliculitis and boils, etc., but there is no corresponding detection method

Method used

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  • Inspection method of streptococcus in cosmetics
  • Inspection method of streptococcus in cosmetics
  • Inspection method of streptococcus in cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] In order to determine whether SCDLP, brain heart infusion medium and sodium azide glucose broth can be used as pre-enrichment solutions for Streptococcus, five standard strains were added to the above three pre-enrichment media, cultured at 37°C, and each Draw 1 mL of culture solution from each enrichment solution every 4 hours, and after ten-fold gradient dilution with sterile normal saline, select the appropriate dilution and apply it to the brain heart infusion agar, and count the colonies. The experiment was carried out continuously for 48 hours. Compare their growth curves to determine the optimal enrichment medium.

[0038] Streptococcus constellation CMCC(B)32116, Streptococcus pyogenes CMCC(B)32002, Streptococcus faecalis CMCC(B)32223, Streptococcus alpha-hemolyticus CMCC(B)32205 and Streptococcus lactis were selected as the standard bacteria, respectively. It was made into a 0.5 McFarland concentration of bacteria, diluted ten times with sterile normal saline, ...

Embodiment 2

[0042] To determine whether blood agar, brain heart infusion agar and KF agar are suitable as isolation media for streptococci. The 5 standard strains with the same amount and appropriate concentration were respectively inserted into blood agar, brain heart infusion agar and KF agar, counted after culturing at 37°C for 24 hours, and observed the growth characteristics and number of growth in the three agar media. , to determine the optimal isolation medium for streptococci.

[0043] Streptococcus constellation CMCC(B)32116, Streptococcus pyogenes CMCC(B)32002, Streptococcus faecalis CMCC(B)32223, Streptococcus alpha-hemolyticus CMCC(B)32205 and Streptococcus lactis were selected as the standard bacteria, respectively. It was made into a bacterial solution of 0.5 McFarland concentration, diluted ten times with sterile physiological saline, and 1 mL was coated on blood agar, brain heart infusion agar and KF agar, cultivated at 37 °C for 24 hours and counted, and counted each sta...

Embodiment 3

[0047] Example 3: Isolation of Streptococcus by Artificial Pollution of Cosmetics

[0048] Streptococcus constellation CMCC(B)32116, Streptococcus pyogenes CMCC(B)32002, Streptococcus faecalis CMCC(B)32223, Streptococcus alpha-hemolyticus CMCC(B)32205 and Streptococcus lactis were selected as the standard bacteria, respectively. It is made to be adjusted to 0.5 McFarland concentration, and mixed into 6 kinds of cosmetics (skin rejuvenation lotion (liquid), skin softening lotion (liquid), facial cleanser (ointment), moisturizing lotion (emulsion semi-emulsion) according to the amount of 1%. solid), nutritional cream (oily), perfume (aerosol and organic solvent)), mix well. The fully mixed liquid, semi-solid and other samples were prepared with reference to GB7918.1-87 (General Principles of Microbiological Standard Test Methods for Cosmetics). Take 10 mL of the 1:10 diluted sample and inoculate it into 90 mL of SCDLP liquid medium, set it in a 37 °C incubator, and cultivate fo...

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Abstract

The invention discloses a method for testing streptococcus in cosmetics, against that no method for testing the streptococcus in the cosmetics is reported at home or abroad. The method identifies and determines spoilage microorganisms of the streptococcus mainly existing in the cosmetics through adopting SCDLP as pre-enriched liquid, blood agar and brain heart infusion agar as an isolation medium through catalase tests and vancocin series biochemical tests. The method is significant to safety of the cosmetics.

Description

Field of Invention [0001] The invention relates to the technical field of cosmetic microorganism testing. Specifically, the present invention relates to the technical field of bacteria detection in cosmetics. Background technique [0002] Cosmetics are widely used in people's daily life, so the detection and evaluation of their safety is particularly important. Cosmetics provide a good nutritional environment for the growth and reproduction of microorganisms due to the presence of many nutritious ingredients such as oil, protein, starch, vitamins, and water in the raw materials. Although cosmetic deterioration is prevented by adding preservatives, microbial contamination cannot be avoided during production and use. Microorganisms metabolize and decompose the nutrients in cosmetics, resulting in spoilage of cosmetics, which not only changes the color, aroma, taste and dosage form of cosmetics, but also causes serious harm to the health of users. Therefore, the separation a...

Claims

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Application Information

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IPC IPC(8): C12Q1/14
Inventor 黄玲蒋刚强刘小兰田延河窦辉季新成
Owner 新疆出入境检验检疫局检验检疫技术中心
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