M-sporangiocyst bacterium Q5-1 for purifying chromium pollution in wastewater and uses thereof

A technology of metasporangium and chromium pollution is applied in the field of environmental microorganisms to achieve the effect of reducing the harm of chromium

Inactive Publication Date: 2009-05-13
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the defects of the existing chromium pollution purification technology in wastewater, and obtain a novel chromium-reducing bacterium, which can redu

Method used

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  • M-sporangiocyst bacterium Q5-1 for purifying chromium pollution in wastewater and uses thereof
  • M-sporangiocyst bacterium Q5-1 for purifying chromium pollution in wastewater and uses thereof
  • M-sporangiocyst bacterium Q5-1 for purifying chromium pollution in wastewater and uses thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Example 1 Isolation and identification of chromium-reducing bacteria

[0027] (1) Sample collection: A soil sample from a manganese mine in Hunan Province, China was collected in early July 2007.

[0028] (2) Sample enrichment: Accurately weigh 100g of soil sample into a 250ml sterilized Erlenmeyer flask, add 5ml of 100mM K 2 CrO4 , stir gently and culture in a 37°C incubator for a week, pay attention to add sterile water to ensure that the sample is not dry.

[0029] (3) Isolation of chromium-resistant bacteria: Accurately weigh K 2 CrO 4 10g of the enriched soil sample was placed in a conical flask filled with 90ml of sterile normal saline, placed in a shaker at 37°C for half an hour, and then 1ml was gradually diluted to 10ml in 9ml of sterile normal saline. -4 , 10 -5 , 10 -6 , respectively take 0.1ml coating containing 1mM K 2 CrO 4 LB solid medium plate, spread 3 plates for each dilution, culture in a 37°C incubator for one week, the grown strains are chrom...

Embodiment 2

[0037] The chromium reduction curve of embodiment 2 Metacystis Q5-1

[0038] Pick a single clone of Metacystis Q5-1 and inoculate it into 100ml LB liquid medium, place it in a shaker at 37°C for shaking culture, and take samples at intervals to measure the cell density to OD 600 It is about 0.15, stored in a refrigerator at 4°C, and used as a seed bacterial solution for inoculation. Draw 1ml into 100ml fresh LB liquid medium with 1% inoculum size, add 1ml 100mM K 2 CrO 4 , so that the final concentration was 1 mM, and placed in a shaker at 37°C for shaking culture. Sampling was started 8 hours after the start of culture, and samples were taken every 2 hours until K 2 CrO 4 until fully restored. Take 1.2ml and 1.5ml respectively each time, and measure the cell density (OD 600 ) and hexavalent chromium concentration (OD 540 ), all are taken and made now. The specific method is as follows: First, measure the cell density (OD 600 ), with deionized water as a reference, di...

Embodiment 3

[0039] Example 3: The cumulative reduction effect of Metacystis Q5-1 on hexavalent chromium in simulated chromium-polluted water

[0040] Deionized water plus K 2 CrO 4 It was used to simulate chromium-polluted water bodies, and to investigate the cumulative removal effect of Metacystis Q5-1 on hexavalent chromium. The specific method is as follows: Prepare three 250ml Erlenmeyer flasks as three repetitions, each filled with 100ml LB medium (121°C, autoclaved for 30 minutes), and absorb 1ml of cell density OD with 1% inoculum 600 For the inoculation of the Q5-1 bacterial solution of about 0.15, add 1ml of 100mM K 2 CrO 4 The mother solution was adjusted to a final concentration of 1 mM, and placed in a shaker at 37°C for shaking culture. Start to culture for 12 hours and begin to take samples for measurement until the K added for the first time 2 CrO 4 Almost completely reduced; then add 1ml 100mM K again 2 CrO 4 Mother solution, so that the final concentration is 1mM,...

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Abstract

The invention belongs to the technical field of environmental microorganisms, and particularly relates to Intrasporangiaceae sp Q5-1 with reduction effect on hexavalent chromium and application of the Intrasporangiaceae sp Q5-1 in the purification of chromium pollution in wastewater. The invention is characterized in that the Intrasporangiaceae sp Q5-1 which has reduction effect on the hexavalent chromium is obtained through the separation from manganese mine soil in Hunan province. The strain can reduce the hexavalent chromium in wastewater containing chromium into trivalent chromium, which greatly reduces the toxicity of the chromium in the wastewater. The strain is named the Intrasporangiaceae sp Q5-1, and is a newly discovered chromium-reducing bacterium, and CCTCC NO of the strain is M208239. The preliminary study shows that the strain has better application prospect in the treatment of the chromium pollution in the wastewater.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, and in particular relates to the screening of a metacystis Q5-1 strain capable of reducing inorganic hexavalent chromium and its use in purifying chromium-containing wastewater pollution. Background technique [0002] At present, heavy metal pollution has become a global problem. In heavy metal pollution, chromium is a pollutant with strong toxicity of heavy metals. Its treatment and recovery have become the focus of scientists from all over the world to study heavy metal pollution. Chromium is one of the commonly used metals in industry. It is mainly used in electroplating and special smelting. In addition, chromium salts can also be used as industrial saddles, pigments, catalysts, fungicides, wood preservatives, etc. With the continuous development of world industry, more and more chromium (VI) is discharged into the natural environment, causing serious environmental pollut...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/22
Inventor 王革娇杨锦霞何敏艳
Owner HUAZHONG AGRI UNIV
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