Plate-type fluorometric enzyme immunoassay for peroxidase antibody and use thereof

A peroxidase and luciferase technology, applied in the field of medical ultra-trace detection, can solve the problems of staying, low specificity and sensitivity, and achieve the effects of convenient operation, high sensitivity and specificity, and easy clinical application.
CN101430332AInactive Publication Date: 2009-05-13GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
1 Cites 1 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
Publication Date
2009-05-13
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to a peroxydase antibody plate type fluorescence enzyme lmmunoassay and the application thereof. The method is characterized in that human thyroid peroxydase (hTPO) protein is taken as antigens to cover a perforated plate, and PBST containing 1-5 percent BSA is used for sealing the perforated plate; the perforated plate is then taken as a carrier and is added with an anti-hTPO first antibody or a standard, an enzyme-labeled second antibody, fluorogenic substrate and stop solution in sequence; and finally, the fluorogenic strength is detected on a fluorogenic / chemical luminescent analyzer. The method can be used to clinically distinguish autoimmunity thyropathy from non-autoimmunity thyropathy and diagnosis lymphocytic thyroiditis. The invention has the advantages that the perforated plastic plate is taken as a solidoid carrier to carry out the fluorescence enzyme lmmunoassay, the plate material has easy sources, low cost and easy popularization; the method is special, sensitive and stable; the detection value is calibrated according to national standard with favorable accuracy and the method is applicable to clinically requirement and popularization.
Need to check novelty before this filing date? Find Prior Art

Description

【Technical field】

[0001] The invention belongs to the technical field of medical ultra-trace detection, and uses a porous plate as a carrier to prepare a solid-phase antigen microplate plate for the establishment and clinical application of a fluorescent enzyme immunoassay (FEIA) for quantitative detection of human thyroid peroxidase (hTPO) antibody . 【Background technique】

[0002] Autoimmune thyroid disease (AITD) is a group of frequently-occurring diseases, mainly including Hashimoto's thyroiditis (HT), Graves' disease (GD), postpartum thyroiditis (PPTD) and so on. Its pathogenesis is affected by various factors such as genetic background, environmental conditions, and immune system, and is manifested as abnormal thyroid function, accompanied by the production of various thyroid autoantibodies, such as thyroid peroxidase antibody (hTPOAb), thyrotropin-affected antibody (TRAb), thyroglobulin antibody (TgAb), etc.

[0003] hTPO and hTPOAb play an important role in the pat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More