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Oleaginous yeast strain and method for preparing biological oil using the same

A yeast strain and bio-oil technology, applied in the biological field, can solve the problems of poor arable land resources and limited arable land.

Inactive Publication Date: 2009-05-20
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, my country's existing arable land resources are poor, and the arable land that can be used to develop energy crops is very limited. It is not in line with my country's national conditions to rely on planting oil crops to provide oil sources for biodiesel

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A kind of method utilizing oleaginous yeast strain to prepare biological oil

[0049] The medium used to ferment and produce bio-oil is a pretreatment solution pretreated with dilute acid. The components are as follows (measured by high performance liquid chromatography): glucose 3.79g / l, xylose 21.27g / l, acetic acid 3.98g / l, furfural 1.14 g / l, hydroxymethylfurfural 0.45g / l. pH 5.0, sterilized at 115°C for 20 minutes. After sterilization, add inorganic salt, the composition is (NH 4 ) 2 SO 4 0.1%~1%, KH 2 PO 4 0.09~0.12%; MgSO 4 ·7H 2 O0.04% ~ 0.06%.

[0050] Pick a ring of colony from the plate and insert it into the seed medium, cultivate it for 18-24 hours at 30° C. and 180 RPM, and insert the above-mentioned sulfuric acid pretreatment solution with 10% inoculum amount. The culture time is 4 days. Sampling and analysis every 24 hours showed that the metabolism of glucose was completed in 48 hours, the metabolism of acetic acid was completed in 72 hours, a...

Embodiment 2

[0053] A kind of method utilizing oleaginous yeast strain to prepare biological oil

[0054] The medium for fermentation to generate bio-oil is the enzymatic hydrolyzate after steam expansion pretreatment. The enzyme used is Accellerase; the cellulase activity is 67.8FPU, and the cellobiase activity is 152IU. The components of the hydrolyzate components are as follows (measured by high performance liquid chromatography): 40g / L of glucose, 9.5g / L of xylose, 0.32g / L of acetic acid, furfural, and the content of hydroxymethylfurfural is very little and can be ignored. 6.0, sterilized at 115°C for 20 minutes.

[0055] Select a ring of colony from the plate and insert it into the seed medium, culture at 30° C., 180 RPM for 24 hours, and insert the above enzyme hydrolyzate with 10% inoculum size. The incubation time is 3 days. The amount of grease finally generated was 3.61g / L, the oil content reached 42.17%, and the grease coefficient (the grease coefficient refers to the amount ...

Embodiment 3

[0057] A kind of method utilizing oleaginous yeast strain to prepare biological oil

[0058] The medium used for fermentation to generate bio-oil is the hydrolyzate obtained by enzymatic hydrolysis after steam expansion pretreatment. The enzyme used is Accellerase; the cellulase activity is 67.8FPU, and the cellobiase activity is 152IU. The components of the hydrolyzed solution are as follows (measured by high performance liquid chromatography): 40g / L of glucose, 9.5g / L of xylose, 0.32g / L of acetic acid, furfural, and the content of hydroxymethylfurfural is very little and can be ignored, and the pH 6.0, sterilized at 115°C for 20 minutes.

[0059] Select a ring of colony from the plate and insert it into the seed medium, culture at 30° C., 180 RPM for 24 hours, and insert the above enzyme hydrolyzate with 10% inoculum size. After culturing for 3 days, the cells were harvested, and all these cells were put back into the hydrolyzate under the above conditions, and a total of ...

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Abstract

The invention relates to an oil-producing yeast strain and a method for preparing biological grease; the oil-producing yeast strain is Trichosporon cutaneum CX1 with the collection No. of CGMCC No. 2527; the method for producing the biological grease by using the oil-producing yeast is as follows: hydrolyzate of stalks is generated after pretreatment and the hydrolysis of crop stalks; a basic inorganic salt component is added in the hydrolyzate, and the oil-producing yeast can utilize sugar and other organic matters in the hydrolyzate for growth and metabolism, thereby generating the grease; the generated grease is stored in cells, and then the biological grease is obtained by extracting the grease in the cells. The invention has the advantages that the composition of the generated grease is similar to vegetable oil, and the grease can be used as the raw material for producing biodiesel; compared with the existing other processes, the invention greatly expands the sources of the grease raw material of the biodiesel and significantly reduces the costs of the raw material grease of the biodiesel.

Description

【Technical field】 [0001] The invention relates to the field of biotechnology, in particular to an oleaginous yeast strain and a method for preparing bio-oil. The related strain is Trichosporon cutaneum CX1, and the preservation number is CGMCC No.2527. 【Background technique】 [0002] With the increasing shortage of oil resources, rising oil prices, increasing contradictions between supply and demand of oil products, and more prominent environmental pollution problems, it is inevitable to develop renewable oil resources through multiple channels. Biodiesel has excellent properties such as high energy density, low sulfur content, sufficient combustion, and good lubricity. It also has the characteristics of renewable, easy biodegradation, safe storage and transportation, and good antiknock performance. It can be used as a high-quality substitute for petrochemical diesel . [0003] The raw materials for preparing biodiesel are mainly vegetable oils, and the cost of raw material...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P7/64C12R1/645
Inventor 鲍杰陈曦
Owner EAST CHINA UNIV OF SCI & TECH
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