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Maackia amurensis extract as well as extracting method and use thereof

A technology of extract, ethanol, applied in the field of medicine

Active Publication Date: 2009-07-08
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no commercially available Sophora japonica extract, so developing a simple and feasible method to obtain Sophora japonica extract for the preparation of estrogenic drugs can fill in the gaps in the field about the extraction and application of Sophora japonica

Method used

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  • Maackia amurensis extract as well as extracting method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Take 1Kg of dry bark of Sophora japonica, crush it, and pass through a 20-mesh sieve. Add 15L of 60% ethanol and reflux extraction at 90°C for 3 times, each time for 1.5 hours. The extract was filtered, and the filtrate was concentrated under reduced pressure into a viscous extract (130 g). Suspend the extract with 300ml of 4M hydrochloric acid and hydrolyze at 90°C for 4 hours. Add 300 ml of ethyl acetate to the hydrolyzate for extraction, separate the ethyl acetate layer, repeat the above operation 3 times, and combine the ethyl acetate layers. Concentrate the ethyl acetate layer to dryness under reduced pressure to obtain the total isoflavone extract of Sophora japonica, wherein the content of total isoflavone aglycone is 31.2%.

Embodiment 2

[0028] Take 1Kg of dry bark of Sophora japonica, crush it, and pass through a 20-mesh sieve. Add 20L of 80% ethanol to soak at room temperature and extract 3 times, 3 days each time. The extract was filtered, and the filtrate was concentrated under reduced pressure into a viscous extract (100 g). Suspend the extract with 300ml 4M acetic acid and hydrolyze at 80°C for 6 hours. 300 ml of dichloromethane was added to the hydrolyzate for extraction, the dichloromethane layer was separated, the above operation was repeated 3 times, and the dichloromethane layers were combined. The dichloromethane layer was concentrated to dryness under reduced pressure to obtain the total isoflavone extract of Sophora japonica, wherein the content of total isoflavone aglycone was 26.4%.

Embodiment 3

[0030] Take 1Kg of dry bark of Sophora japonica, crush it, and pass through a 20-mesh sieve. Add 15L of 60% ethanol and reflux extraction at 90°C for 3 times, each time for 1.5 hours. The extract was filtered, and the filtrate was concentrated under reduced pressure into a viscous extract (130 g). Suspend the extract with 400ml of pH6 potassium phosphate buffer, add 800U / g of β-glucosidase, stir and hydrolyze at 36°C for 3 hours. Add 400 ml of ethyl acetate to the hydrolyzate for extraction, separate the ethyl acetate layer, repeat the above operation 3 times, and combine the ethyl acetate layers. Concentrate the ethyl acetate layer to dryness under reduced pressure to obtain the total isoflavone extract of Sophora japonica, wherein the content of total isoflavone aglycone is 35.7%.

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Abstract

The invention relates to a Maackia amurensis extract and the application thereof. The Maackia amurensis extract is prepared by the method including the following steps: heart wood, root cortices, stem barks, branch barks, leaves, leaf stalks, flowers, fruits and / or seeds of the Maackia amurensis are extracted by methanol or ethanol; the extracts are filtered and are concentrated and dried into extractum or dry powder; the extractum or the dry powder is catalyzed and hydrolyzed by inorganic acid, organic acid or enzyme; acid hydrolysate or enzymatic hydrolysate is obtained through extraction by aliphatic ester, methylene dichloride or methenyl chloride; and organic phase is concentrated to be dry to obtain the Maackia amurensis extract. The total content of isoflavone glycoside of the Maackia amurensis extract prepared by the method ranges from 15 percent to 85 percent. The invention also uses the Maackia amurensis extract as an estrogenic drug for preventing the breast cancer, the prostatic cancer, the colon cancer, the osteoporosis and the climacteric syndrome.

Description

Technical field: [0001] The invention belongs to the technical field of medicine, and relates to an extract of pagoda japonica and its extraction method and application, in particular to the extract of pagoda japonica and its extraction method and its application in the preparation of estrogen drugs. Background technique: [0002] North Korean locust tree (Maackia amurensis Rupr. et Maxim.), also known as mountain locust tree, Korean locust tree, and Huai locust tree, is a plant of the genus Saddle Tree in the family Fabaceae. The whole plant of Sophora japonica contains a variety of flavonoid compounds such as formoononetin, genistein, afromosin, afromosin, irisin, gehuating, etc. It is one of the few plants containing 4 kinds of isoflavones (phytoestrogens) and its glycosides. [0003] Isoflavones have weak estrogen activity and can bind to estrogen receptors. Depending on individual hormone status, isoflavones can exert estrogen-like or anti-estrogen activity, showing a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48A61P5/30A61K125/00A61K127/00A61K129/00A61K131/00A61K133/00A61K135/00
Inventor 崔征黎雄王东张会梅代英辉
Owner SHENYANG PHARMA UNIVERSITY
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