Method for preparing hydroxylapatite-silk fibroin compound stent material using two-step method
A technology of hydroxyapatite and silk fibroin, which is applied in medical science, prosthesis, etc., can solve the problems of poor interfacial bonding force, easy damage of interfacial layer, loss of effective strength, etc., and achieve the effect of good mechanical properties.
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[0018] Example 1
[0019] (1) Add 100 mL of CaCl with a concentration of 0.25 mol / L to 10 mL of a 2.5% (w / v) silk fibroin solution. 2 Solution and 100mL NaH with a concentration of 0.15mol / L 2 PO 4 The solution is constantly stirred, and the pH of the solution is adjusted to 9 during the reaction. After the dripping is completed, the reaction solution is aged for 24h, washed and centrifuged (8000rpm, 5min) three times to obtain a hydroxyapatite-silk fibroin composite slurry;
[0020] (2) Add a 5% (w / v) concentration of silk fibroin solution to the hydroxyapatite-silk fibroin composite slurry and stir continuously. The dry weight of the composite slurry and the mass ratio of silk fibroin are 4:1 , Adjust the pH to 9; after stirring for 20-30 minutes, inject the mixture into the mold, pre-frozen at -20°C for 12h, freeze-dry at -80°C for 2h, and then freeze-dry to obtain a porous hydroxyapatite-silk fibroin composite scaffold material.
Example Embodiment
[0021] Example 2
[0022] (1) In 25mL of 1% (w / v) Bombyx mori silk fibroin solution, 100mL of Ca(NO 3 ) 2 Solution and 100mL Na with a concentration of 0.15mol / L 2 HPO 4 The solution is constantly stirred, and the pH of the solution is adjusted to 9 during the reaction. After the dripping is completed, the reaction solution is aged for 24h, washed and centrifuged (8000rpm, 5min) three times to obtain a hydroxyapatite-silk fibroin composite slurry;
[0023] (2) Add a 2% (w / v) silk fibroin solution to the hydroxyapatite-silk fibroin composite mud and stir continuously. The dry weight of the composite mud and the mass ratio of silk fibroin are 4:1 , Adjust the pH to 9; after stirring for 20-30 minutes, inject the mixture into the mold, pre-frozen at -20°C for 12h, freeze-dry at -80°C for 2h, and then freeze-dry to obtain a porous hydroxyapatite-silk fibroin composite scaffold material.
Example Embodiment
[0024] Example 3
[0025] (1) In 50 mL of the silk fibroin solution of 0.5% (w / v), 100 mL of Ca(NO 3 ) 2 Solution and 100mL K with a concentration of 0.15mol / L 2 HPO 4 The solution is constantly stirred, and the pH of the solution is adjusted to 9 during the reaction. After the dripping is completed, the reaction solution is aged for 24h, washed and centrifuged (8000rpm, 5min) three times to obtain a hydroxyapatite-silk fibroin composite slurry;
[0026] (2) Add 8% (w / v) silk fibroin solution to the hydroxyapatite-silk fibroin composite slurry and stir continuously. The dry weight of composite slurry and the mass ratio of silk fibroin are 4:1 , Adjust the pH to 9; after stirring for 20-30 minutes, inject the mixture into the mold, pre-frozen at -20°C for 12h, freeze-dry at -80°C for 2h, and then freeze-dry to obtain a porous hydroxyapatite-silk fibroin composite scaffold material.
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