Method for preparing hydroxylapatite-silk fibroin compound stent material using two-step method

A technology of hydroxyapatite and silk fibroin, which is applied in medical science, prosthesis, etc., can solve the problems of poor interfacial bonding force, easy damage of interfacial layer, loss of effective strength, etc., and achieve the effect of good mechanical properties.

Active Publication Date: 2009-07-08

ZHEJIANG SCI-TECH UNIV

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AI-Extracted Technical Summary

Problems solved by technology

However, according to relevant literature reports, the interfacial binding force between hydroxyapatite and organic matrix is poor. In a physiological environment, the interface layer betwee...

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Abstract

The invention discloses a two-step method for preparing hydroxyapatite-silk fibroin composite stent material, comprising the following steps: (1) under the environment of alkaline solution, chemical bonding is formed by carboxyl on silk fibroin molecular and Ca ions in the solution; when PO4 irons are dripped, the Ca ions react with the PO4 irons to generate the hydroxyapatite, therefore, hydroxyapatite-silk fibroin composite slurry is prepared; (2) after silk fibroin solution is added in the hydroxyapatite-silk fibroin composite slurry, the intermolecular hydrogen bonding is formed between silk fibroin molecular in the composite and silk fibroin molecular in the solution, then, the mixed solution is poured in a die to obtain the porous hydroxyapatite-silk fibroin composite stent material after freezing-freeze drying. Compared with hydroxyapatite-silk fibroin composite stent material which is directly prepared by a prior blending method, the stent material prepared by the invention has perfect mechanical property.

Application Domain

Prosthesis

Technology Topic

IonFreeze dry +12

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Examples

Experimental program(5)
Comparison scheme(1)

Example Embodiment

[0018] Example 1

[0019] (1) Add 100 mL of CaCl with a concentration of 0.25 mol/L to 10 mL of a 2.5% (w/v) silk fibroin solution. 2 Solution and 100mL NaH with a concentration of 0.15mol/L 2 PO 4 The solution is constantly stirred, and the pH of the solution is adjusted to 9 during the reaction. After the dripping is completed, the reaction solution is aged for 24h, washed and centrifuged (8000rpm, 5min) three times to obtain a hydroxyapatite-silk fibroin composite slurry;

[0020] (2) Add a 5% (w/v) concentration of silk fibroin solution to the hydroxyapatite-silk fibroin composite slurry and stir continuously. The dry weight of the composite slurry and the mass ratio of silk fibroin are 4:1 , Adjust the pH to 9; after stirring for 20-30 minutes, inject the mixture into the mold, pre-frozen at -20°C for 12h, freeze-dry at -80°C for 2h, and then freeze-dry to obtain a porous hydroxyapatite-silk fibroin composite scaffold material.

Example Embodiment

[0021] Example 2

[0022] (1) In 25mL of 1% (w/v) Bombyx mori silk fibroin solution, 100mL of Ca(NO 3 ) 2 Solution and 100mL Na with a concentration of 0.15mol/L 2 HPO 4 The solution is constantly stirred, and the pH of the solution is adjusted to 9 during the reaction. After the dripping is completed, the reaction solution is aged for 24h, washed and centrifuged (8000rpm, 5min) three times to obtain a hydroxyapatite-silk fibroin composite slurry;

[0023] (2) Add a 2% (w/v) silk fibroin solution to the hydroxyapatite-silk fibroin composite mud and stir continuously. The dry weight of the composite mud and the mass ratio of silk fibroin are 4:1 , Adjust the pH to 9; after stirring for 20-30 minutes, inject the mixture into the mold, pre-frozen at -20°C for 12h, freeze-dry at -80°C for 2h, and then freeze-dry to obtain a porous hydroxyapatite-silk fibroin composite scaffold material.

Example Embodiment

[0024] Example 3

[0025] (1) In 50 mL of the silk fibroin solution of 0.5% (w/v), 100 mL of Ca(NO 3 ) 2 Solution and 100mL K with a concentration of 0.15mol/L 2 HPO 4 The solution is constantly stirred, and the pH of the solution is adjusted to 9 during the reaction. After the dripping is completed, the reaction solution is aged for 24h, washed and centrifuged (8000rpm, 5min) three times to obtain a hydroxyapatite-silk fibroin composite slurry;

[0026] (2) Add 8% (w/v) silk fibroin solution to the hydroxyapatite-silk fibroin composite slurry and stir continuously. The dry weight of composite slurry and the mass ratio of silk fibroin are 4:1 , Adjust the pH to 9; after stirring for 20-30 minutes, inject the mixture into the mold, pre-frozen at -20°C for 12h, freeze-dry at -80°C for 2h, and then freeze-dry to obtain a porous hydroxyapatite-silk fibroin composite scaffold material.

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Description & Claims & Application Information

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Classification and recommendation of technical efficacy words

Good mechanical properties

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Mixed solution Slurry Freeze-drying Mechanical property Stent Physical chemistry

Method for preparing hydroxylapatite-silk fibroin compound stent material using two-step method

A technology of hydroxyapatite and silk fibroin, which is applied in medical science, prosthesis, etc., can solve the problems of poor interfacial bonding force, easy damage of interfacial layer, loss of effective strength, etc., and achieve the effect of good mechanical properties.

AI-Extracted Technical Summary

Problems solved by technology

Abstract

Image

Examples

Example Embodiment

Example Embodiment

Example Embodiment

PUM

Description & Claims & Application Information

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