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Method for preparing hydroxylapatite-silk fibroin compound stent material using two-step method

A technology of hydroxyapatite and silk fibroin, which is applied in medical science, prosthesis, etc., can solve the problems of poor interfacial bonding force, easy damage of interfacial layer, loss of effective strength, etc., and achieve the effect of good mechanical properties.

Active Publication Date: 2009-07-08
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, according to relevant literature reports, the interfacial binding force between hydroxyapatite and organic matrix is ​​poor. In a physiological environment, the interface layer between hydroxyapatite and polymer matrix is ​​easily damaged, which leads to the failure of the composite material before the defect is completely repaired. prematurely loses its effective strength

Method used

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  • Method for preparing hydroxylapatite-silk fibroin compound stent material using two-step method
  • Method for preparing hydroxylapatite-silk fibroin compound stent material using two-step method
  • Method for preparing hydroxylapatite-silk fibroin compound stent material using two-step method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Add 100 mL of CaCl with a concentration of 0.25 mol / L dropwise in the silk fibroin protein solution of 10 mL with a concentration of 2.5% (w / v) 2 solution and 100 mL of NaH with a concentration of 0.15 mol / L 2 PO 4 The solution was stirred continuously, and the pH value of the solution was adjusted to be 9 during the reaction. After the dropwise addition, the reaction liquid was aged for 24 hours, washed and centrifuged (8000rpm, 5min) three times to obtain hydroxyapatite-silk fibroin composite mud;

[0020] (2) Add silkworm silk fibroin protein solution with a concentration of 5% (w / v) to the hydroxyapatite-silk fibroin composite mud and keep stirring. The mass ratio of the dry weight of the composite mud to silk fibroin is 4:1 , adjust the pH value to 9; after stirring for 20 to 30 minutes, pour the mixture into the mold, pre-freeze at -20°C for 12 hours, freeze at -80°C for 2 hours, and freeze-dry to prepare a porous hydroxyapatite-silk fibroin composite scaffo...

Embodiment 2

[0022] (1) Add 100 mL of 0.25 mol / L Ca(NO 3 ) 2 solution and 100 mL of Na with a concentration of 0.15 mol / L 2 HPO 4 The solution was stirred continuously, and the pH value of the solution was adjusted to be 9 during the reaction. After the dropwise addition, the reaction liquid was aged for 24 hours, washed and centrifuged (8000rpm, 5min) three times to obtain hydroxyapatite-silk fibroin composite mud;

[0023] (2) Add silkworm silk fibroin solution with a concentration of 2% (w / v) to the hydroxyapatite-silk fibroin composite mud and keep stirring. The mass ratio of the dry weight of the composite mud to silk fibroin is 4:1 , adjust the pH value to 9; after stirring for 20 to 30 minutes, pour the mixture into the mold, pre-freeze at -20°C for 12 hours, freeze at -80°C for 2 hours, and freeze-dry to prepare a porous hydroxyapatite-silk fibroin composite scaffold Material.

Embodiment 3

[0025] (1) Add 100 mL of 0.25 mol / L Ca(NO 3 ) 2 solution and 100 mL of K with a concentration of 0.15 mol / L 2 HPO 4 The solution was stirred continuously, and the pH value of the solution was adjusted to be 9 during the reaction. After the dropwise addition, the reaction liquid was aged for 24 hours, washed and centrifuged (8000rpm, 5min) three times to obtain hydroxyapatite-silk fibroin composite mud;

[0026] (2) Add silkworm silk fibroin solution with a concentration of 8% (w / v) to the hydroxyapatite-silk fibroin composite mud and keep stirring, the mass ratio of the dry weight of the composite mud to silk fibroin is 4:1 , adjust the pH value to 9; after stirring for 20 to 30 minutes, pour the mixture into the mold, pre-freeze at -20°C for 12 hours, freeze at -80°C for 2 hours, and freeze-dry to prepare a porous hydroxyapatite-silk fibroin composite scaffold Material.

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Abstract

The invention discloses a two-step method for preparing hydroxyapatite-silk fibroin composite stent material, comprising the following steps: (1) under the environment of alkaline solution, chemical bonding is formed by carboxyl on silk fibroin molecular and Ca ions in the solution; when PO4 irons are dripped, the Ca ions react with the PO4 irons to generate the hydroxyapatite, therefore, hydroxyapatite-silk fibroin composite slurry is prepared; (2) after silk fibroin solution is added in the hydroxyapatite-silk fibroin composite slurry, the intermolecular hydrogen bonding is formed between silk fibroin molecular in the composite and silk fibroin molecular in the solution, then, the mixed solution is poured in a die to obtain the porous hydroxyapatite-silk fibroin composite stent material after freezing-freeze drying. Compared with hydroxyapatite-silk fibroin composite stent material which is directly prepared by a prior blending method, the stent material prepared by the invention has perfect mechanical property.

Description

technical field [0001] The invention relates to the technical field of material preparation, in particular to a two-step method for preparing a hydroxyapatite-silk fibroin composite support material. Background technique [0002] Bone tissue in the body is a biomineralized tissue that grows under the control of cell height regulation, and is composed of two major parts—organic matter and inorganic matter. Organic matter (mainly collagen fiber bundles) acts as a bone network scaffold, endowing bone with elasticity and toughness; while inorganic matter (mainly hydroxyapatite crystals) determines the hardness and rigidity of bone. At the same time, hydroxyapatite (HAP) has good biocompatibility and osteoconduction, and is one of the bone repair / replacement materials with good performance. However, materials prepared from a single hydroxyapatite have low strength and poor toughness, and cannot be widely used as bone repair / replacement materials. Therefore, the preparation of h...

Claims

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Application Information

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IPC IPC(8): A61L27/46A61L27/22A61L27/12
Inventor 姚菊明吴娟红沈凯蔡玉容
Owner ZHEJIANG SCI-TECH UNIV
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