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High yield adenomethionine strain

An adenosylmethionine, high-yield technology, applied in the field of microorganisms, can solve problems such as low efficiency of strain screening

Inactive Publication Date: 2011-02-09
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to address the above defects in the prior art, provide a high-yielding adenosylmethionine strain and its screening method, improve the yield of adenosylmethionine, and solve the problem of low strain screening efficiency in the adenosylmethionine production process

Method used

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Examples

Experimental program
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Embodiment 1

[0058] Screening of High-Adenosylmethionine-producing Strains

[0059] The screening method of the present invention is a screening method for ultraviolet mutation breeding, and the steps are as follows: prepare Saccharomyces cerevisiae suspension; get 3mL of bacterial suspension to a sterilized plate with a diameter of 6cm, and magnetically stir; 15W, 30cm away from ultraviolet radiation; take 0.5mL After ultraviolet irradiation, the bacterial solution was spread to the slant medium of a sulfur-deficient plate containing 150 μg / ml ethionine; cultured at 30°C in the dark for 36 hours. The mutant strains with ethionine resistance were selected, the feedback inhibition of adenosylmethionine production was removed, cultured in shake flasks, the ability of adenosylmethionine production was detected, and the strains with high adenosylmethionine production ability were screened out.

[0060] 1. Experimental Preparation

[0061] (1) Prepare medium:

[0062] Shake flask medium: gluc...

Embodiment 2

[0072] The preparation method of adenosylmethionine

[0073]1. Fermentation culture: Get the Saccharomyces cerevisiae strain (SAM0238) screened in Example 1, and after activation, cultivate it to the logarithmic growth phase through shaking flasks to obtain a fermented seed liquid. Inoculate the fermented seed liquid with 5% inoculation amount into the fermentation medium and cultivate it for 24 hours to obtain the fermentation liquid. The culture condition is 220rpm, 30°C. 4 NO 3 , 0.08% KH 2 PO 4 , 0.03% CaCl 2 , 0.016%K 2 HPO 4 , 0.01% MgSO 4 7H2O, 0.04% FeSO 4 ·7H 2 O, pH 5.0, the liquid volume in a 500mL Erlenmeyer flask is 100mL.

[0074] 2. Fermentation broth pretreatment: take 1L of the mixed fermentation broth, mix the fermentation broth with 1.5mol / L perchloric acid in a volume ratio of 1:2, bathe in 40°C water for 30min, centrifuge at 4000r / min for 15min, and collect the supernatant.

[0075] 3. Ion exchange adsorption and elution: add 2% Reye's salt to th...

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Abstract

The invention relates to the technical field of microorganism, in particular to a high-yielding ademetionine strain and a screening method thereof. The screened high-yielding ademetionine strain is named as SAM0238; the colony of the strain is milky white, lustrous and flat and has smooth edges; the classification name thereof is Saccharomyces cerevisiae; the collection number is CGMCC No.2842; and the collection unit is China General Microbiological Culture Collection Center(CGMCC). The strain can produce the high-yielding ademetionine. The invention further discloses a method for preparing ademetionine by using the strain.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a high-yield adenosylmethionine strain and a screening method thereof, and also discloses a method for preparing adenosylmethionine by using the strain. Background technique [0002] S-adenosylmethionine is also called active methionine, the English name is S-adenosylmethionine, and the abbreviation is SAM, SAMe or AdoMet. Its chemical structural formula is as follows: [0003] [0004] It is an intermediate metabolite that widely exists in organisms and has important physiological activities. Its importance in cell metabolism is second only to adenosine triphosphate (ATP). As an important physiologically active substance, it participates in the methylation of body metabolism and is closely related to many key body functions including gene regulation, hormonal activity, nerve conduction development and detoxification. Studies have shown that SAM has obvious curative e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/40C12N1/16C12R1/865
Inventor 周长林杨明华李登奎窦洁
Owner CHINA PHARM UNIV
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