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Compositional bactericidal agent for preventing and treating gray mold

A fungicide and gray mold technology, applied in the field of compound fungicides for the prevention and treatment of gray mold, can solve the problems of tomato production threat, yield loss, fruit rot, etc., achieve low production cost, reduce use cost, and expand the control spectrum Effect

Inactive Publication Date: 2009-07-22
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Botrytis cinerea is an important disease worldwide, which mainly causes fruit rot and yield loss
Great threat to tomato production in protected areas

Method used

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  • Compositional bactericidal agent for preventing and treating gray mold
  • Compositional bactericidal agent for preventing and treating gray mold
  • Compositional bactericidal agent for preventing and treating gray mold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1. Determination of cross-resistance between cyproxazole and chlorothalonil

[0020] 1. Test strains

[0021] The tested strains were from Shandong, Henan, Beijing, Tianjin, Nanjing and other areas where cyproxazole and other sterol synthesis inhibitors have not been used. Including 11 strains of sensitive strains, 6 strains of natural field mutants and 28 strains of UV-induced drug-resistant mutants. A total of 45 strains were used for cross-drug resistance determination.

[0022] The above-mentioned strains were obtained as follows:

[0023] In May 2006, tomato gray mold disease fruits were collected in greenhouses that have not used cyproxazole and similar agents (other sterol synthesis inhibitors) in Beijing, Nanjing, Shandong, Henan and Tianjin. The 5-point sampling method is used to reduce the chance of collecting the same strain. Use a sterilized scalpel to remove the rotten skin of the diseased fruit, take the fresh diseased fruit pulp, cut it into small pie...

Embodiment 2

[0041] Example 2: Determination of combined toxicity of cyproxazole and chlorothalonil compound

[0042] 1. Test strains

[0043] In Example 1, a strain of B. cinerea that is sensitive to pyriconazole was selected, and a strain of B. cinerea that was resistant to pyriconazole was resistant to B. cinerea. The medicinal strains were tested for joint virulence determination.

[0044] 2. Test medicine

[0045] The test agents were 92% pyriconazole original drug and 90% chlorothalonil original drug. The mass ratios of cyproxazole single dose and chlorotoxin list reagent are 1:0 (single dose of cyproxazole), 1:1, 1:2, 1:5, 1:8, 2:1, 5: Compounding in the ratio of 1, 8:1, 0:1 (chlorophyll list agent).

[0046] 3. Test medium

[0047] YG medium (5g yeast powder, 18g glucose, 12.5g agar powder, 1000ml deionized water), sterilized.

[0048] 4. Experimental method

[0049] The virulence was determined by the mycelial growth rate method.

[0050] 4.1 Configuration of liquid medicine

[0051] ...

Embodiment 3

[0088] Example 3 Field plot disease prevention experiment of pyriconazole and chlorothalonil compound preparation

[0089] The test crop variety: tomato, the variety is Hezuo 908 (produced by the Shanghai Long March Fine Variety Experimental Site, and sold commercially). This variety is of mid-early maturity type, infinite type, vigorous plant growth, wide application area for cultivation, and strong disease and stress resistance.

[0090] The experiment set up 7 treatments: I: 25% pyriconazole EC and 75% chlorothalonil wettable powder compound formulation, in which the mass ratio of pyriconazole and chlorothalonil is 1:2, diluted by 600 times Liquid spraying; II: 25% cyproxazole EC and 75% chlorothalonil wettable powder compound preparation, wherein the mass ratio of cyproxazole and chlorothalonil is 1:2, diluted by 1250 times Carry out spraying; III: 25% pyriconazole EC and 75% chlorothalonil wettable powder compound preparation, in which the mass ratio of pyriconazole and chlor...

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Abstract

The invention discloses a compounded fungicide for controlling gray mold, which has gnotobiotic oxazole and chlorothalonil as active components of which the mass ratio is (1-8):(1-8), preferably 1:1, 1:2 or 5:1 and 8:1, and most preferably 1:1 or 1:2. The novel systemic fungicide gnotobiotic oxazole with a single action site and the protective fungicide chlorothalonil of the compounded fungicide with a multi-action site are compounded so that the compounded fungicide has important meanings for avoiding or retarding the generation of the field drug fastness and enlarging the controlling spectrum of the creative fungicide gnotobiotic oxazole in China, can reduce the use cost of the gnotobiotic oxazole simultaneously, and has potential economic meanings.

Description

Technical field [0001] The invention relates to a compound fungicide for preventing and treating gray mold. Background technique [0002] Tomato gray mold is a worldwide important disease, which mainly causes fruit rot and yield loss. It poses a great threat to tomato production in protected areas. Since the disease began to spread in my country in the 1980s, it has now occurred in various parts of the country, and it has become a restrictive obstacle to tomato cultivation. The pathogen of the disease is Botrytiscinerea Pers ex Fr (anamorph of Botryotinia fuckeliana (de Bary) Whetz), which is a fungus deuteromycotina. The bacteria can infect at least 235 kinds of plants in temperate regions, and can cause gray mold disease of a variety of important economic crops (including vegetables, flowers, bulbs and fruit trees, especially grapes). [0003] The control measures for B. cinerea mainly include agricultural control, biological control and chemical control. As the occurrence of g...

Claims

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Application Information

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IPC IPC(8): A01N43/80A01N37/34A01P3/00
Inventor 刘西莉刘敏李健强黄中乔韩平陈风平
Owner CHINA AGRI UNIV
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