Non-contact stationary type fluorescent molecular tomography method and device

Abstract

The invention relates to a noncontact fixed fluorescence molecular tomography method and device, and is characterized in that an imaging device comprising an excitation light source, an optical switch part, a plurality of light transmitting optical fibers, a plurality of optical lenses, an image transmitting optical fiber and a detector is arranged; the plurality of the light transmitting optical fibers and the plurality of the optical lenses are respectively arranged around an object to be detected; the optical switch part is controlled by a computer; excitated light emitted by the excitation light source irradiates on the object to be detected through any light transmitting optical fiber; fluorescence excitated by the object to be detected is imaged on each forky end of the image transmitting optical fiber through each optical lens and then is transmitted to the detector arranged on the light outlet of the image transmitting optical fiber through the composite end of the image transmitting optical fiber; the excitated light is switched to the light transmitting optical fibers at different positions through the optical switch part in turn; the fluorescence signal acquisition process is repeated to realize 360-degree full-angle fluorescence signal on the boundary of the object to be detected. The noncontact fixed fluorescence molecular tomography method and device can realize 360-degree full-angle and noncontact fixed acquisition of the fluorescence signal of the object to be detected, and has high imaging efficiency and quality.

Description

technical field [0001] The invention relates to a fluorescent molecular imaging method and device, in particular to a non-contact fixed fluorescent molecular tomographic imaging method and device. Background technique [0002] Fluorescence molecular tomography (Fluorescence molecular tomography, also known as fluorescence molecular tomography or fluorescence diffusion optical tomography) uses specific fluorescent molecules that have been widely used in biological and medical research as probes for labeling Specific molecules or cells, which observe the changes of molecular levels in organisms in vivo, and provide information such as the distribution of targets through image reconstruction, thereby overcoming the limitations of planar imaging, and obtaining more information about biological and medical behaviors. information. Fluorescence molecular tomography has the advantages of high sensitivity, quick and easy, low cost, and relatively high throughput. It not only support...

AI Technical Summary

Problems solved by technology

[0005] In the existing non-contact fluorescent molecular tomography, the CCD camera 114 and the excitation light source 113 must be rotated relative to the experimental animal body 111 to obtain a 360° full-angle fluorescent signal on the boundary of the experimental animal body 111. If the system imaging time is long, Not only does it affect the survival of the experimental animal body, it is not conducive to the construction of small animal models in the body for a long period of time, but also the experimental imaging efficiency is low, and it is difficult to achieve high-throughput experiments, which limits the application of fluorescence molecular tomography

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