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Application of Ampelopsis grossedentata total flavone in preparing medicament for preventing and treating adverse reaction of tumor chemoradiotherapy

A technology that reveals the adverse reactions of snake grapes and is applied in the direction of antineoplastic drugs, drug combinations, and pharmaceutical formulas, and can solve problems such as macromolecule function damage, inactivation, and physical decline

Inactive Publication Date: 2009-07-22
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemotherapy drugs and radiotherapy can fight tumors, but they all produce some adverse reactions more or less, and it is difficult to control and eliminate adverse reactions, especially body mutations and secondary tumors, etc.
During radiotherapy and chemotherapy, chemotherapy drugs induce free radicals in the body, or themselves can be transformed into chemical structures in the form of free radicals, attacking cell membrane lipids, proteins and nucleic acid molecules, destroying the conformation and structural stability of these macromolecules, resulting in macromolecules The function is damaged or gradually inactivated; due to the functional inactivation of macromolecular proteins and the breakage of the linear structure of nucleic acids (DNA and RNA), gene mutations occur, cell repair functions are severely reduced, and the accumulation of gene mutations will trigger new secondary Tumor; organ emergency response function and cell stress repair ability are reduced, and the accumulation of chemical toxic side effects will lead to tissue and organ damage, such as bone marrow suppression and leukopenia, resulting in decreased immunity and complicated infection is difficult to control, hair follicle and dermis damage leading to hair loss or Rash, digestive tract and digestive organs are often more susceptible to the toxic effects of cytotoxic chemotherapy drugs, causing anorexia, vomiting, nausea, severe decline in physical strength, etc.
[0006] Tumor patients generally have low immunity, and their ability to repair the emergency response and toxic effects of exogenous chemical substances is also very low. Or prevent and treat adverse reactions of tumor drug treatment, but rarely produce adverse drug reactions or toxic side effects of new anti-tumor drugs

Method used

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  • Application of Ampelopsis grossedentata total flavone in preparing medicament for preventing and treating adverse reaction of tumor chemoradiotherapy
  • Application of Ampelopsis grossedentata total flavone in preparing medicament for preventing and treating adverse reaction of tumor chemoradiotherapy
  • Application of Ampelopsis grossedentata total flavone in preparing medicament for preventing and treating adverse reaction of tumor chemoradiotherapy

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: Extraction and separation preparation of THT

[0018] Extraction process method: add the mixture of dried young leaves and buds of the plant of Snake dentatus to 1:8-10 weight ratio (raw material: water), add purified water, heat to 70°C and soak for 0.5-1 hour , heated to boiling, heat preservation and extraction for 30-60 minutes, then tangential flow ultrafiltration with ceramic membrane (30KD cut-off) while hot, to collect the extract; add again according to the weight ratio of 1:5-1:8 (dry material: water) Purified water, heated to boiling, heat-preserved and extracted for 20-40 minutes, tangential flow ultrafiltration with ceramic membrane (30KD cut-off) while hot, combined and collected extracts; concentrated extracts under reduced pressure to 20%-40% of the original volume % (or the relative density is 1.06-1.10), add edible alcohol until the final concentration of ethanol is 50%-75%, heat to boiling and reflux for 20-60 minutes, press filter or ce...

Embodiment 2

[0019] Embodiment 2: the colorimetric method determination of the flavonoid content of THT

[0020] 1. Principle: The phenolic hydroxyl group in flavonoids can interact with Al 3+ The ions form a yellow complex with a characteristic absorption wavelength of 314nm. There is a linear relationship between color concentration and flavonoid content within a certain range.

[0021] 2. Instruments and Reagents

[0022] Analytical balance, sensitivity 0.0001g; UV-visible spectrophotometer;

[0023] Anhydrous methanol (AR); 5% AlCl3 solution; dihydromyricetin (control standard).

[0024] 3. Detection method

[0025] (1) Preparation of the standard solution: put the standard dihydromyricetin in a weighing dish, dry it at 105°C to constant weight, take it out and let it cool naturally in a desiccator, weigh 0.0504g accurately immediately, dissolve it in methanol, and constant volume to 50ml.

[0026] (2) Preparation of standard concentration gradient sample solution: draw the above...

Embodiment 3

[0030] Example 3: The inhibitory effect of THT on the formation of methemoglobin caused by tertiary peroxide (TBHP)

[0031] 1. Experimental method

[0032] Add packed erythrocytes to double distilled water to make 1% erythrocytes completely hemolyzed, add each sample component and incubate at 37°C for 30min, add TBHP to make the final concentration reach 250uM, continue to incubate for 30min, measure OD 630 Relative changes in methemoglobin content were detected. Calculation of high ferritin production inhibition rate:

[0033] Generation inhibition rate=(OD of oxidative damage group 630 - Sample group OD 630 ) / (Oxidative damage group OD 630 - control group OD 630 )

[0034] 2. Experimental results

[0035] TBHP releases H gradually in the aqueous phase 2 o 2 , H 2 o 2 Catalyzed by free or bound iron in heme to produce superoxide anion and hydroxyl radicals through the Fenton reaction. The maximum light absorption values ​​of the α and β peptide chains of normal ery...

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Abstract

The invention discloses application of total flavone of ampolopsis grossedentata in preparing medicines for preventing and treating untoward reaction in tumor radiation treatment and chemo-treatment, and in particular relates to application of the total flavone of ampolopsis grossedentata in preparing medicines for preventing and treating the untoward reaction in tumor radiation treatment and chemo-treatment, preventing and treating arrest of bone marrows and alopecia, having anti-mutagenic function, preventing and treating generation of secondary tumor and tumor metastasis, and preventing and treating breast carcinoma, cervical carcinoma, intestinal carcinoma and the like. The invention creatively finds out that the total flavone of ampolopsis grossedentata eliminates free radicals, inhibits free radical reaction chains, uses THT for anti-mutation, inhibits tumor gene expression and initiates tumor cell apoptosis so as to prevent and treat tumor generation, secondary tumor and tumor metastasis, uses the THT to inhibit virus transfection so as to prevent and treat infection, and realizes prevention, control or elimination of hazards or toxic hazards of chemical medicines and radiation on histiocyte and organs.

Description

technical field [0001] The present invention relates to the new medical application of the total flavonoids of S. chinensis, especially the application of the total flavonoids of Sn. Drugs or health food to prevent tumor occurrence and tumor metastasis, prevent and treat concurrent infectious diseases in tumor treatment. Background technique [0002] A. grossedentata (A. grossedentata) is a plant of the genus Vitis vinifera. Its young stems, leaves and buds are processed and dried to become white tea, also known as vine tea, Tianpo tea, etc. White tea in the Guangdong and Guangxi regions includes Cantonese snake grape (A·cantoniesis) in addition to the toothed snake grape. [0003] Snake-toothed snake grapes are widely distributed in Guangdong, Guangxi, Yunnan, Hunan, Hubei, Jiangxi and other provinces and regions in my country. The content of THT in the dry weight of young stems and leaves is more than 20%, which is the highest content of physiologically active component m...

Claims

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Application Information

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IPC IPC(8): A61K36/87A61P7/00A61P17/14A61P43/00A61P35/00
Inventor 张晓元郭勇
Owner SOUTH CHINA UNIV OF TECH
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