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Blood platelet additive solution and preparation method thereof

A technology for adding liquid and platelets, applied in the fields of biology and medicine, can solve the problems of high platelet activation rate and poor preservation effect of apheresis platelets, and achieve the effects of preventing aggregation, maintaining preservation quality, and inhibiting activation.

Active Publication Date: 2009-07-22
江苏省血液中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is: aiming at the high activation rate of platelets existing in the use of the current platelet additive solution, the in vitro preservation quality is lower than 100% plasma medium, which can only replace 60-70% of the plasma medium, and has poor preservation effect for apheresis platelets And other issues

Method used

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  • Blood platelet additive solution and preparation method thereof
  • Blood platelet additive solution and preparation method thereof
  • Blood platelet additive solution and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation 1 of platelet additive solution (take the preparation of 1000ml as an example):

[0029] Preparation of raw materials:

[0030] Sodium chloride 4.67g; magnesium chloride 0.41g; potassium chloride 0.38g; sodium citrate 4.41g; sodium acetate 2.05g; glucose 2.97g; sodium dihydrogen phosphate 0.63g; sodium bicarbonate 1.09g; L-arginine 30mg; (the above raw materials are pharmacopoeia grade, the same below), sufficient double distilled water.

[0031] One 1000ml container, one measuring cup, one glass stirring rod, one 1000ml sterile medical plastic bag, one 0.22μm sterile filter.

[0032] Preparation Process:

[0033] The ambient temperature is 21°C.

[0034] Put the pharmacopoeia-grade drug into a container, add 800ml of double-distilled water, stir until the color becomes clear, continue to add double-distilled water to adjust to 1000ml, stir evenly to form a medicinal liquid; filter the medicinal liquid with a 0.22 μm sterile filter To sterility, obtain 100...

Embodiment 2

[0036] Preparation 2 of platelet additive solution (take the preparation of 1000ml as an example):

[0037] Preparation of raw materials:

[0038] Sodium chloride 4.95g; magnesium chloride 0.38g; potassium chloride 0.41g; sodium citrate 4.21g; sodium acetate 2.15g; glucose 3.60g; sodium dihydrogen phosphate 0.57g; sodium bicarbonate 0.98g; L-arginine 25mg; enough double distilled water.

[0039] One 1000ml container, one 500ml container, one measuring cup, one glass stirring rod, one 1000ml sterile medical plastic bag, one 100ml sterile medical plastic bag, and one flexible packaging ventilated dry heat sterilizer.

[0040] Preparation Process:

[0041] The ambient temperature is 24°C.

[0042] Put sodium chloride, magnesium chloride, potassium chloride, sodium citrate, sodium acetate, sodium bicarbonate, and L-arginine in the raw materials into a 1000ml container, add 800ml double distilled water, and continue to add double distilled water after dissolving Adjust the dist...

Embodiment 3

[0044] Application of the platelet additive solution obtained in Example 1 in the collection of concentrated platelets from multiple people

[0045] Implementation process:

[0046] Separation of the buffy coat (BC) layer of a single blood unit: take 400ml of raw whole blood collected in a quadruple bag, centrifuge at a centrifugal force of 3450g for 10min in a centrifuge at a temperature of 22°C±2°C, and centrifuge the platelet-rich The buffy coat layer (containing platelets, red blood cells, and white blood cells) is extruded into a sub-bag, and the extruded volume is 40ml to 45ml. After heat sealing, it is separated from the mother bag, and the extruded buffy coat is left standing overnight at 22°C±2°C. .

[0047] Adding liquid to collect the buffy coat layer to prepare multiple people’s concentrated platelets: collect 6 bags of buffy coat with the same ABO and RH blood types into the same plasma bag with sterile connection technology, and then add 200-220ml of platelet ad...

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Abstract

The invention relates to a platelet additive solution and a preparation method thereof. 1,000 milliliters of the platelet additive solution comprises the following compositions: 4.21 to 5.14 grams of sodium chloride, 0.84 to 1.26 grams of sodium bicarbonate, 0.37 to 0.45 gram of magnesium chloride, 3.97 to 4.85 grams of sodium citrate, 1.85 to 2.25 grams of sodium acetate, 0.45 to 0.75 gram of sodium dihydrogen phosphate, 0.34 to 0.42 gram of potassium chloride, 22 to 35 milligrams of L-arginine and 1.80 to 3.60 grams of glucose, wherein the pH value of the additive solution is between 7.2 and 7.4. The preparation method comprises: weighing the officinal sodium chloride, magnesium chloride, potassium chloride, sodium citrate, the officinal sodium acetate, glucose, sodium dihydrogen phosphate, sodium bicarbonate and L-arginine respectively according to the compositions; and dissolving the raw materials into proper amount of redistilled water to prepare liquid medicine, performing sterilization treatment, and storing the liquid medicine by aseptic medical plastic bags at normal temperature.

Description

technical field [0001] The invention relates to the fields of biology and medicine, in particular to a platelet additive solution and a preparation method thereof. Background technique [0002] Platelet transfusion is mainly a platelet transfusion replacement therapy for patients with thrombocytopenia or abnormal platelet function, in order to achieve the purpose of hemostasis or prevention of bleeding. There are two main types of platelet products used clinically: manual platelet concentrate and platelet apheresis. At present, the conventional platelet storage method is 20℃~24℃ normal temperature shaking storage, the storage medium is plasma, and the number of platelets in each therapeutic dose of platelet products is ≥2.5×10 11 / L, in a platelet storage bag containing 250ml~300ml of plasma, the storage period is 5 days. For example, using crystal salt solution instead of plasma medium to preserve platelets not only saves precious plasma resources, but also reduces many a...

Claims

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Application Information

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IPC IPC(8): A61K47/26A61K9/08A61P7/04A61K47/16A61K47/12A61K47/02
Inventor 黄成垠唐荣才魏鹏肖健宇胡政芳肖国锋
Owner 江苏省血液中心
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