Production method of guanine nucleosides

Abstract

The invention discloses a guanosine production method, which consists of four major technical processes including fluid strain cultivation in a shaking bottle, production of glucose solution, production of guanosine fermented fluid and extraction and refinement of guanosine, adopts a preserved strain TA208-IMPD with genetic stability, prepares the glucose solution by steeping, pulping, liquefying, filter pressing and saccharifying cracked grains, adopts new techniques including flow feeding of the glucose solution in a fermentation process, and produces qualified guanosine through microfiltration membrane sterilization, crude guanosine crystallization, ultrafiltration membrane decoloring and impurity removing, separating and parching of the guanosine fermented fluid. The guanosine production method overcomes the disadvantages of traditional new techniques that have behindhand culture techniques, lower guanosine yield of the strain, long fermentation period, lower transformation ratio and lower synthesized extraction yield, and the like, achieves high synchronized production efficiency and guanosine production level of the strain, stable glucose solution product and high guanosine fermented production level, and can reach the guanosine product purity of over 99 percent and the yield of over 80 percent.

Description

technical field [0001] The invention belongs to a production method of guanosine, an important intermediate of food and medical products. Background technique [0002] Guanosine (trade name: GR) is also known as 9-β-D-ribofuranosylguanine, referred to as guanosine, and its English name is Guanosine. Guanosine is white crystalline powder or off-white crystalline powder, soluble in water and pyrimidine, insoluble in alcohol. Molecular formula is C 10 h 13 h 5 o 5 , the relative molecular weight is 283.24. The chemical structural formula is as follows: [0003] [0004] Guanosine has a wide range of uses and is an important intermediate in food and pharmaceutical products. In the field of pharmaceutical industry, guanosine is mainly used as an important intermediate for the synthesis of nucleoside antiviral drugs such as ribavirin and acyclovir, and is also used to manufacture acyclovir (Acyclovir), ribavirin ( ATC), sodium guanosine triphosphate (GTP), the anticance...

AI Technical Summary

Problems solved by technology

In the 1960s, Japan carried out large-scale research on the production of guanosine by fermentation, mainly focusing on traditional mutation breeding techniques to change the characteristics of strains and increase the yield of guanosine, up to 20g / L. less research

The main reason is that the breeding technology is backward, the glycoside production level of the strain is not high; the fermentation cycle is long, the conversion rate and the comprehensive yield of extraction are low

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