32 results about "Guanosine triphosphate" patented technology

The invention discloses a small G-protein Rop family protein, a coding gene and an application of the hevea brasiliensis latex small G-protein Rop family protein. The protein is a protein having one of the following amino acid residue sequences: 1) protein composed of an amino acid residue sequence of SEQ ID No.1 in a sequence table; 2) SEQ ID No.1 derived protein obtained by subjecting the amino acid residue sequence of SEQ ID No.1 in the sequence table to substitution and / or deletion and / or addition of one or more amino acid residues and having GTP (guanosine triphosphate) binding function. The protein has GTP binding activity, plays a certain role in basic disease-resistant mechanism and stress resistance of plants, and has the characteristics of control for secondary xylem and phloem development of woods. The protein can improve the resistance of transgenic crop or woods against adverse situation, so as to improve the yield.

The invention provides methods to inhibit the replication of a flavivirus, methods of inhibiting the guanosine triphosphate (GTP)-binding and guanylyltransferase activity of a flavivirus RNA capping enzyme, and methods of treating a subject infected with a flavivirus. The methods can include contacting a flavivirus with an effective amount of a thioxothiazolidine compound described herein, or a derivative thereof, such as a compound of Formula (I).

The invention discloses a method for testing BNP (Brain Natriuretic Peptide) activity by using a GCA stable transfection cell line and establishes a novel BNP bioactivity testing method. GCA which is a main acceptor of BNP is an adenylate cyclase precursor and can catalyze GTP (guanosine triphosphate) to generate cGMP after being activated by the BNP and then exerts a biological effect. Wield 293 cell has low GCA expression level and low reactivity to BNP. In the method disclosed by the invention, a stable cell line 293GCAC3 with BNP acceptor (GCA) overexpression level is structured by transgenosis, the content change of cGMP secreted into cell supernatant by the 293GCAC3 stimulated by the BNP is detected to quantify the activity of the BNP. The method comprises the following specific testing steps of: laying cells on a 96-pore plate and culturing for 16-18 hours; adding the BNP for stimulating for 90 minutes; and sucking cell supernatant and detecting the content of cGMP with competitive ELISA (enzyme-linked immuno sorbent assay). The method is simple to operate, has sensitive response and small variability and is significant for the quality control and clinical application of the BNP.