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32 results about "Guanosine triphosphate" patented technology
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Guanosine-5'-triphosphate (GTP) is a purine nucleoside triphosphate. It is one of the building blocks needed for the synthesis of RNA during the transcription process. Its structure is similar to that of the guanine nucleobase, the only difference being that nucleotides like GTP have a ribose sugar and three phosphates, with the nucleobase attached to the 1' and the triphosphate moiety attached to the 5' carbons of the ribose.
Blocking induction of tetrahydrobioterin to block induction of nitric oxide synthesis
InactiveUS6153615AReduce inhibitionRestore sensitivityBiocidePeptide/protein ingredientsSide effectTreatment effect
Guanosine triphosphate pathway tetrahydrobiopterin synthesis antagonist and / or pterin salvage pathway tetrahydrobiopterin synthesis antagonists are administered to inhibit nitric oxide synthesis from arginine in vascular cells in a subject in need of such inhibition (e.g., for prophylactic or curative effect for endotoxin- or cytokine-induced hypotension or for restoration of vascular contractile sensitivity to pressor agents in the treatment of such hypotension). The tetrahydrobiopterin synthesis antagonist may be administered with alpha 1-adrenergic agonist or with nitric oxide synthase inhibitor. The tetrahydrobiopterin synthesis antagonists are also administered to attenuate inflammation caused by induced nitric oxide production in immune cells. Unwanted counterproductive or side effects can be eliminated or ameliorated by administration additionally of levodopa with or without carbidopa and L-5-hydroxytryptophane.
Owner:CORNELL RES FOUNDATION INC
Diguanylate cyclase gene, vector, engineering bacteria and application
InactiveCN104152472ABacteriaMicroorganism based processesEscherichia coliRecombinant escherichia coli
The invention provides a diguanylate cyclase gene for coding diguanylate cyclase, a recombinant vector containing the gene, recombinant gene engineering bacteria obtained by transformation of the recombinant vector, and application thereof in the preparation of recombinant diguanylate cyclase. The diguanylate cyclase can be bonded with an expression vector to construct intracellular expression recombinant plasmid containing the genes for respectively correspondingly transforming to escherichia coli to obtain recombinant escherichia coli. The recombinant escherichia coli containing the recombinant diguanylate cyclase can be used as an enzyme source for biological catalysis and transformation. The recombinant diguanylate cyclase as a transformation enzyme can use 5'-guanosine triphosphate (GTP) trisodium as a substrate for transformation for preparation of cyclic di-guanylic monophosphate (c-di-GMP).
Owner:陶飞
Reagent, method and kit for detection of biological activity of glucagon-like peptide-1 (GLP-1)
ActiveCN103344773ASmall batch-to-batch varianceEasy to purifyReceptors for hormonesBiological testingDipeptidyl peptidaseMethyl xanthine
The invention relates to a reagent for detection of biological activity of glucagon-like peptide-1 (GLP-1). Each 100 microliters of the reagent mainly comprises 20 micrograms of a gene recombinant GLP-1 acceptor, a dipeptidyl peptidase-4 inhibitor of which the weight is 1% of the weight of a blood plasma sample, 480 micrograms of triphosadenine, 10 micrograms of guanosine triphosphate, 0.5 milligrams of 3-isobutyl-1-methylxanthine and 100 microliters of a membrane reaction solution. The GLP-1 acceptor is obtained by genetic recombination, is convenient for purification and reduces batch difference of the reagent. The GLP-1 acceptor is used as a probe for capturing GLP-1 so that detection sensitivity is improved, biological activity can be directly determined, sensitivity is high, and practicality is strong.
Owner:斯坦姆(天津)生物技术研究有限公司
Reagent, method and kit for detection of biological activity of glucagon-like peptide-1 (GLP-1)
The invention relates to a reagent for detection of biological activity of glucagon-like peptide-1 (GLP-1). The reagent added into each 15 microliters of a detection sample comprises: 1, 35-45 microliters of a membrane reaction solution, 2, 15-25 microliters of a GLP-1 acceptor, 3, 10-20 microliters of a GLP-1 standard substance, 4, 5-15 microliters of sitgliptin phosphate, 5, 1-10 microliters of triphosadenine, 6, 1-10 microliters of guanosine triphosphate, 7, 5-15 microliters of 3-isobutyl-1-methylxanthine, 8, 1-10 microliters of bovine serum albumin, 9, 50-150 microliters of a biotin-labeled mouse anti-human cAMP monoclonal antibody, 10, a microwell plate coated with a goat anti-human cAMP polyclonal antibody, 11, 50-150 microliters of an avidin-labeled horseradish peroxidase, 12, 300-400 microliters of a PBS washing liquor, 13, 50-150 microliters of a chromogenic substrate, and 14, 50-150 microliters of a stop buffer. The GLP-1 acceptor is produced by genetic recombination, is convenient for purification and can reduce the batch difference of the reagent. The GLP-1 acceptor is used as a probe for capturing GLP-1 so that detection sensitivity is improved, biological activity can be directly determined, sensitivity is high, and practicality is strong.
Owner:MEIDE TAIPINGYANG PRECISION INSTR MFG
Guanosine triphosphate-binding protein coupled receptors
InactiveUS20070009916A1Efficient extractionQuality improvementCell receptors/surface-antigens/surface-determinantsSugar derivativesHuman DNA sequencingBioinformatics
The object of the present invention is to provide a technique for efficiently extracting GPCR sequences from human genome sequences, thereby comprehensively identifying novel GPCRs. An original automatic system for identifying GPCR sequences is disclosed, and 1035 novel GPCRs are successfully identified from the entire human genome by utilizing the system.
Owner:NAT INST OF ADVANCED IND SCI & TECH
High-energy substance for preventing and treating neurodegenerative disease of old people and medical application of substance
InactiveCN103520190ABoost energy levelsReasonable treatmentOrganic active ingredientsNervous disorderS-Adenosyl-l-methionineHigh energy
The invention relates to a high-energy substance for preventing and treating a neurodegenerative disease of old people. The high-energy substance comprises one or more of compounds and derivatives of the compounds of phosphoenolpyruvic acid, creatine phosphoric acid, acetyl coenzyme A, adenosine triphosphoric acid, L-carnitine, s-adenosylmethionine, guanosine triphosphate, cytidine triphosphate, pyrophosphoric acid, 1,3-diphosphoglycerate and succinyl coenzyme A. The high-energy substance has the benefits as follows: the high-energy substance is used for treating the neurodegenerative disease of the old people, particularly the aged population, so as to delay and prevent dementia; and the high-energy substance is proved to be a novel medicine which is reasonable, safe and economical and used for treating the neurodegenerative disease, particularly senile dementia.
Owner:陈铭
Thioxothiazolidine inhibitors
ActiveUS20140004590A1Inhibit the GTP binding and guanylation activityHigh activityOrganic active ingredientsSsRNA viruses positive-senseBiochemistryGuanylyltransferase activity
The invention provides methods to inhibit the replication of a flavivirus, methods of inhibiting the guanosine triphosphate (GTP)-binding and guanylyltransferase activity of a flavivirus RNA capping enzyme, and methods of treating a subject infected with a flavivirus. The methods can include contacting a flavivirus with an effective amount of a thioxothiazolidine compound described herein, or a derivative thereof, such as a compound of Formula (I).
Owner:THE STATE OF COLORADO ACTING BY & ON BEHALF OF THE BOARD OF TRUSTEES OF THE UNIV OF NORTHERN COLORADO +1
Hevea brasiliensis latex small G-protein Rop family protein and coding gene thereof
InactiveCN102731638AImprove stress resistanceIncrease resistanceBacteriaPlant peptidesProteinGuanosine triphosphate
The invention discloses a small G-protein Rop family protein, a coding gene and an application of the hevea brasiliensis latex small G-protein Rop family protein. The protein is a protein having one of the following amino acid residue sequences: 1) protein composed of an amino acid residue sequence of SEQ ID No.1 in a sequence table; 2) SEQ ID No.1 derived protein obtained by subjecting the amino acid residue sequence of SEQ ID No.1 in the sequence table to substitution and / or deletion and / or addition of one or more amino acid residues and having GTP (guanosine triphosphate) binding function. The protein has GTP binding activity, plays a certain role in basic disease-resistant mechanism and stress resistance of plants, and has the characteristics of control for secondary xylem and phloem development of woods. The protein can improve the resistance of transgenic crop or woods against adverse situation, so as to improve the yield.
Owner:RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
Process for the enzymatic production of cyclic diguanosine monophosphate employing a diguanylate cyclase comprising a mutated rxxd motif
A process is disclosed for the production of cyclic di-guanosine monophosphate (c-di-GMP) without the use of protecting groups by means of an enzymatic synthesis. The process comprises the coupling of two guanosine triphosphate (GTP) molecules so as to form a c-di-GMP molecule. This is done under the influence of a mutant diguanylate cyclase (DGC) comprising the amino acid sequence V153M154G155G156. It has been found that the DGC is obtainable from inclusion bodies, and therewith can be made available in amounts sufficient to improve the c-di-GMP synthesis. Particularly, the latter synthesis can be conducted in a one-pot method starting from commercially available bulk chemicals and allows upscaling to a commercial production scale.
Owner:INTERVET INT BV
Small G protein Rop (Rho-related GTPase from plant) family protein of Hevea brasiliensis latex and coding gene thereof
InactiveCN102731639ARegulation generationRegulate metabolismFungiBacteriaSmall G ProteinGenetically modified crops
The invention discloses a coding gene of small G protein Rop (Rho-related GTPase from plant) and application. The protein has one of the following amino acid residual sequences: 1) amino acid residual sequence of SEQ ID NO.1 in a sequence table; and 2) protein which has an amino acid residual sequence of SEQ ID NO.1 in the sequence table by substitution and / or loss and / or addition of one or more amino acid residues, has the GTP (Guanosine Triphosphate) binding function and is derived from SEQ ID NO.1. The protein has GTP binding activity, can regulate synthesis and metabolization of the active gron ROS, praticifate seuv, participates in stress response, has the characteristics of regulating development of the secondary xylem and phloem of woods, and can be used for improving the resistance of the protein in an adverse situation in genetically modified crops or woods, so that the yield can be increased.
Owner:RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
Detection kit based on aptamer
The invention discloses a detection kit based on an aptamer. The detection kit comprises stem-loop structure nucleic acid H1, DNA (deoxyribonucleic acid) 1, DNA2 and DNA3, the stem-loop structure nucleic acid comprises the aptamer divided into two sections, the aptamer serves as a molecular recognition element, specific binding of molecules to be detected is achieved, catalytic activity of DNA enzymes is activated, substrate chains are circularly cut, high fluorescent detection signals are generated, and high-sensitivity detection is achieved. Detection limit of the detection kit for ATP (adenosine triphosphate) is 0.2 nanometer, detected linear range is wide and is 1 nanometer to 10 micrometers, a detection system has excellent specificity, common analogues comprising CTP (cytidine triphosphate), GTP (guanosine triphosphate) and UTP (uridine triphosphate) do not interfere detection results, separation and purification processes are omitted in the detecting process, detection can be achieved by simple blending, the detection kit has the advantages of simplicity in operation, low cost, quick response and the like, and the detection kit can be used for rapidly detecting and analyzingthe content of the ATP in environments or food samples.
Owner:GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
Cell-free protein synthesis method and cell-free protein synthesis reaction solution using adenosine 3',5'-bisphosphate
InactiveUS20090155881A1Enhanced inhibitory effectConveniently suppressing mRNA degradationTransferasesFermentationCreatine kinasePhosphate
The present invention provides a method of conducting cell-free protein synthesis by conveniently suppressing mRNA degradation, and a reaction solution enabling cell-free protein synthesis by conveniently suppressing mRNA degradation. A cell-free protein synthesis method using a cell-free protein synthesis reaction solution containing at least an extract liquid derived from a living cell, a potassium salt, a magnesium salt, adenosine triphosphate, guanosine triphosphate, creatine phosphate, creatine kinase, amino acid, a tRNA, an mRNA, a buffer, and adenosine 3′,5′-bisphosphate.
Owner:SHIMADZU CORP
Method for testing BNP (Brain Natriuretic Peptide) activity by using GCA stable transfection cell line
ActiveCN102174636APromote research and developmentQuality is easy to controlMicrobiological testing/measurementForeign genetic material cellsSorbentTransgene
The invention discloses a method for testing BNP (Brain Natriuretic Peptide) activity by using a GCA stable transfection cell line and establishes a novel BNP bioactivity testing method. GCA which is a main acceptor of BNP is an adenylate cyclase precursor and can catalyze GTP (guanosine triphosphate) to generate cGMP after being activated by the BNP and then exerts a biological effect. Wield 293 cell has low GCA expression level and low reactivity to BNP. In the method disclosed by the invention, a stable cell line 293GCAC3 with BNP acceptor (GCA) overexpression level is structured by transgenosis, the content change of cGMP secreted into cell supernatant by the 293GCAC3 stimulated by the BNP is detected to quantify the activity of the BNP. The method comprises the following specific testing steps of: laying cells on a 96-pore plate and culturing for 16-18 hours; adding the BNP for stimulating for 90 minutes; and sucking cell supernatant and detecting the content of cGMP with competitive ELISA (enzyme-linked immuno sorbent assay). The method is simple to operate, has sensitive response and small variability and is significant for the quality control and clinical application of the BNP.
Owner:NAT INST FOR FOOD & DRUG CONTROL
Process for the enzymatic production of cyclic diguanosine monophosphate employing a diguanylate cyclase comprising a mutated rxxd motif
InactiveUS20110288286A1Better addressSugar derivativesTransferasesEnzymatic synthesisInclusion bodies
A process is disclosed for the production of cyclic di-guanosine monophosphate (c-di-GMP) without the use of protecting groups by means of an enzymatic synthesis. The process comprises the coupling of two guanosine triphosphate (GTP) molecules so as to form a c-di-GMP molecule. This is done under the influence of a mutant diguanylate cyclase (DGC) comprising the amino acid sequence V153M154G155G156. It has been found that the DGC is obtainable from inclusion bodies, and therewith can be made available in amounts sufficient to improve the c-di-GMP synthesis. Particularly, the latter synthesis can be conducted in a one-pot method starting from commercially available bulk chemicals and allows upscaling to a commercial production scale.
Owner:INTERVET INT BV
High temp ersistant triphosphate guanosine cyclohydrolase gene sequence and coded polypeptide and method for preparing said polypeptide
The present invention discloses a high-temp. resistant triphosphoric acid guanosine cyclohydrolase gene, its coded polypeptide and preparation method. It relates to coding the separate DNA with activity or its function-identical variant and producing the polypeptide with high-temp. resistant triphosphoric acid guanosine cyclohydrolase activity or its function-identical variant by DNA recombination technology and said separate DNA. Said ivnention uses the sequencing and analysis of Tengchong thermophilic anaerobe hologenome as basis to clone and separate the high-temp. resistant triphosphoric acid guanosine cyclohydrolase gene, and utilizes said gene to prepare its transgenic microbe or transgenic animal and plant for producing said high-temp. resistant triphosphoric acid guanosine cyclohydrolase and recover the enzyme obtaining said gene code.
Owner:HUADA GENE RES & DEV CENT HANGZHOU
Method for preparing guanosine triphosphate by using immobilized enzyme method
PendingCN110714043AImprove responseReaction is easy to controlImmobilised enzymesTransferasesNucleotideEnzyme catalysis
The invention relates to the technical field of biology and in particular to a method for preparing guanosine triphosphate by using an immobilized enzyme method. The method specifically comprises thefollowing steps: (1) preparing a GTP producing enzyme; (2) immobilizing the GTP producing enzyme; and (3) separating a product. In the method, two GTP producing enzymes including PPK and GMPK are adopted, and GTP can be synthesized by two-step enzymatic reactions; compared with a traditional tedious process for producing GTP by using a beer yeast, the method has the advantages that the reaction process is simpler, a reaction is easier to control, and the quality of a product is more stable; GTP is prepared by adopting an immobilized enzyme catalysis method, and an immobilized enzyme can be continuously and repeatedly used for many times, so that the production cost is greatly reduced; meanwhile, impurities such as pigments and other types of nucleotides introduced by using the yeast are avoided, so that purification is easier to realize; and the method is suitable for producing GTP on a large scale.
Owner:美亚药业海安有限公司
Reagent, method and kit for detection of biological activity of glucagon-like peptide-1 (GLP-1)
ActiveCN103344773BSmall batch-to-batch varianceEasy to purifyReceptors for hormonesBiological testingDipeptidyl peptidaseMethyl xanthine
The invention relates to a reagent for detection of biological activity of glucagon-like peptide-1 (GLP-1). Each 100 microliters of the reagent mainly comprises 20 micrograms of a gene recombinant GLP-1 acceptor, a dipeptidyl peptidase-4 inhibitor of which the weight is 1% of the weight of a blood plasma sample, 480 micrograms of triphosadenine, 10 micrograms of guanosine triphosphate, 0.5 milligrams of 3-isobutyl-1-methylxanthine and 100 microliters of a membrane reaction solution. The GLP-1 acceptor is obtained by genetic recombination, is convenient for purification and reduces batch difference of the reagent. The GLP-1 acceptor is used as a probe for capturing GLP-1 so that detection sensitivity is improved, biological activity can be directly determined, sensitivity is high, and practicality is strong.
Owner:斯坦姆(天津)生物技术研究有限公司
Application of RhoC guanosine triphosphate
The invention sifts the protein with differential expression which is in cancer organises of the hepatocyte cancer and nearby cancer organises to obtain a high expression protein in the cancer organizes of the hepatocyte cancer. The differential expression of the RhoC guanosine triphosphatase between the cancers organises of the hepatocyte cancer and nearby cancer organizes exists indeed is confirmed by the immune imprint experiment. In consideration of the correlation between the RhoC guanosine triphosphatase and the hepatoma, the protein can be used as theprotein molecule mark to detect the hepatocyte cancer.
Owner:SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
Reagent, method and kit for detection of biological activity of glucagon-like peptide-1 (GLP-1)
ActiveCN103344764BSmall batch-to-batch varianceEasy to purifyBiological testingPhosphateMethyl xanthine
The invention relates to a reagent for detection of biological activity of glucagon-like peptide-1 (GLP-1). The reagent added into each 15 microliters of a detection sample comprises: 1, 35-45 microliters of a membrane reaction solution, 2, 15-25 microliters of a GLP-1 acceptor, 3, 10-20 microliters of a GLP-1 standard substance, 4, 5-15 microliters of sitgliptin phosphate, 5, 1-10 microliters of triphosadenine, 6, 1-10 microliters of guanosine triphosphate, 7, 5-15 microliters of 3-isobutyl-1-methylxanthine, 8, 1-10 microliters of bovine serum albumin, 9, 50-150 microliters of a biotin-labeled mouse anti-human cAMP monoclonal antibody, 10, a microwell plate coated with a goat anti-human cAMP polyclonal antibody, 11, 50-150 microliters of an avidin-labeled horseradish peroxidase, 12, 300-400 microliters of a PBS washing liquor, 13, 50-150 microliters of a chromogenic substrate, and 14, 50-150 microliters of a stop buffer. The GLP-1 acceptor is produced by genetic recombination, is convenient for purification and can reduce the batch difference of the reagent. The GLP-1 acceptor is used as a probe for capturing GLP-1 so that detection sensitivity is improved, biological activity can be directly determined, sensitivity is high, and practicality is strong.
Owner:MEIDE TAIPINGYANG PRECISION INSTR MFG
Fermentation method for increasing yield of riboflavin
PendingCN113755551AIncrease productionPromote accumulationBacteriaMicroorganism based processesBiotechnologyGluconic acid
The present invention provides a fermentation method for increasing the yield of riboflavin, the gluconic acid metal complex is added into the fermentation culture medium, fermentation nutrients are fed, the functions of carbohydrates can be diversified, absorption of thalli to metal elements is optimized, and the toxic and side effects of the metal elements to the thalli are reduced; along with the proceeding of fermentation, the nutritional ingredients in the fermentation tank are gradually reduced, the vitality of thalli is gradually weakened, and fed-batch fermentation nutrients can supplement the nutritional ingredients required by the thalli in the fermentation process and maintain the growth vitality of the thalli; due to the addition of citric acid, the EMP pathway is inhibited, and the HMP pathway is enhanced; glutamine enhances the generation of riboflavin precursor guanosine triphosphate, and is beneficial to the accumulation of riboflavin; and the bacillus subtilis is used as a growth coupling type strain for producing riboflavin, the quantity of thalli influences the yield of riboflavin to a great extent, and the fed-batch of KH2PO4 can keep the thalli in a good growth state, so that the yield of riboflavin is increased.
Owner:TIANJIN UNIV OF SCI & TECH
Hevea brasiliensis latex small G-protein Rop family protein and coding gene thereof
InactiveCN102731638BImprove stress resistanceIncrease resistanceBacteriaPlant peptidesGenetically modified cropsBiology
Owner:RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
Thioxothiazolidine inhibitors
ActiveUS9206412B2Inhibit the GTP binding and guanylation activityHigh activityOrganic active ingredientsSsRNA viruses positive-senseBiochemistryGuanylyltransferase activity
The invention provides methods to inhibit the replication of a flavivirus, methods of inhibiting the guanosine triphosphate (GTP)-binding and guanylyltransferase activity of a flavivirus RNA capping enzyme, and methods of treating a subject infected with a flavivirus. The methods can include contacting a flavivirus with an effective amount of a thioxothiazolidine compound described herein, or a derivative thereof, such as a compound of Formula (I).
Owner:THE STATE OF COLORADO ACTING BY & ON BEHALF OF THE BOARD OF TRUSTEES OF THE UNIV OF NORTHERN COLORADO +1
Preparation method of adenylosuccinic acid or adenylosuccinate
ActiveCN108018323ABreak through monopoly salesSimple preparation processSugar derivativesMicroorganism based processesDiseaseAdenylosuccinate
The invention belongs to the technical field of scale production of candidate medicines for treating glucose-lipid metabolism disorder diseases. In order to solve the problems that existing S-AMP is difficultly produced, the price of the S-AMP is expensive, and raw material sources of the druggability of the S-AMP is difficulty ensured, the invention provides a preparation method of adenylosuccinic acid or adenylosuccinate. PhAdSS of which the N terminal is provided with an His-tag sequence is expressed by utilizing a pET-28-a carrier; with the PhAdSS as a catalyst, inosinic acid IMP, L-aspartic acid and guanosine triphosphate (GTP) are catalyzed and subjected to reaction to synthesize S-AMP; after the reaction is carried out, components in a product are analyzed by adopting silica gel thin-layer chromatography, and separation and purification on the S-AMP are carried out by adopting silica gel coluumn chromatography. The content of the s-AMP in a synthetic product is 61 percent, and arecycling ratio is about 20 percent. A preparation process is simple, the cost is low, and a basis is laid for building an industrialized S-AMP synthetic process; monopoly sale on S-AMP by foreign sellers is expected to be broken through, and the S-AMP druggability research cost can be greatly reduced.
Owner:INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
Cell-free translation system, method and product
PendingCN113409887AOvercome inability to expressOvercome the problem of long expression cycle and low efficiencyProteomicsGenomicsProtein targetCell free
The invention relates to the field of molecular biology, in particular to a cell-free translation system, method and product. The translation system comprises mRNA of 5'cap and 3 'polyadenylic acid, an amino acid mixture, an energy molecule and a mixture of mRNA translation enzyme sources; the energy molecule comprises phosphocreatine, ATP (adenosine triphosphate) and guanosine triphosphate (GTP). Through mRNA transcription, in-vitro transcription and gel electrophoresis analysis, the translation system can rapidly obtain a large amount of target proteins and has the following characteristics: an extracellular translation technology is adopted to overcome the problem that a prokaryotic cell expression part gene cannot be expressed; the problems of long expression cycle and low efficiency of eukaryotic cells are solved by adopting an extracellular translation technology; the problems of low activity and poor efficiency of the original extracellular translation technology are solved by adopting a strategy of adding energy micromolecules such as phosphocreatine; and the stability, the receptor binding speed and the overall translation speed are enhanced by adopting mRNA of 5 ' cap and 3' polyadenylic acid.
Owner:INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
Application of GCA stable transfection cell line to test BNP (Brain Natriuretic Peptide) activity
ActiveCN102174636BPromote research and developmentQuality is easy to controlMicrobiological testing/measurementForeign genetic material cellsSorbentTransgene
Owner:NAT INST FOR FOOD & DRUG CONTROL
A detection kit based on nucleic acid aptamer
ActiveCN107653297BVersatilityEasy to operateMicrobiological testing/measurementAptamerMolecular recognition
The invention discloses a detection kit based on an aptamer. The detection kit comprises stem-loop structure nucleic acid H1, DNA (deoxyribonucleic acid) 1, DNA2 and DNA3, the stem-loop structure nucleic acid comprises the aptamer divided into two sections, the aptamer serves as a molecular recognition element, specific binding of molecules to be detected is achieved, catalytic activity of DNA enzymes is activated, substrate chains are circularly cut, high fluorescent detection signals are generated, and high-sensitivity detection is achieved. Detection limit of the detection kit for ATP (adenosine triphosphate) is 0.2 nanometer, detected linear range is wide and is 1 nanometer to 10 micrometers, a detection system has excellent specificity, common analogues comprising CTP (cytidine triphosphate), GTP (guanosine triphosphate) and UTP (uridine triphosphate) do not interfere detection results, separation and purification processes are omitted in the detecting process, detection can be achieved by simple blending, the detection kit has the advantages of simplicity in operation, low cost, quick response and the like, and the detection kit can be used for rapidly detecting and analyzingthe content of the ATP in environments or food samples.
Owner:GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
A kind of preparation method of adenylyl succinic acid or salt
ActiveCN108018323BBreak through monopoly salesSimple preparation processSugar derivativesMicroorganism based processesPhosphoric acidSuccinic acid
The invention belongs to the technical field of scale production of candidate medicines for treating glucose-lipid metabolism disorder diseases. In order to solve the problems that existing S-AMP is difficultly produced, the price of the S-AMP is expensive, and raw material sources of the druggability of the S-AMP is difficulty ensured, the invention provides a preparation method of adenylosuccinic acid or adenylosuccinate. PhAdSS of which the N terminal is provided with an His-tag sequence is expressed by utilizing a pET-28-a carrier; with the PhAdSS as a catalyst, inosinic acid IMP, L-aspartic acid and guanosine triphosphate (GTP) are catalyzed and subjected to reaction to synthesize S-AMP; after the reaction is carried out, components in a product are analyzed by adopting silica gel thin-layer chromatography, and separation and purification on the S-AMP are carried out by adopting silica gel coluumn chromatography. The content of the s-AMP in a synthetic product is 61 percent, and arecycling ratio is about 20 percent. A preparation process is simple, the cost is low, and a basis is laid for building an industrialized S-AMP synthetic process; monopoly sale on S-AMP by foreign sellers is expected to be broken through, and the S-AMP druggability research cost can be greatly reduced.
Owner:INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
Application of guanosine triphosphate cyclohydrolase 1 and drug
ActiveCN110354257AImprove proliferative abilityReduce the effects of lung radiation damagePeptide/protein ingredientsHydrolasesRadiation injuryTherapeutic effect
The invention relates to application of guanosine triphosphate cyclohydrolase 1 (GCH1) and a drug. It is found that GCH1 overexpression has the prevention and treatment effect on radiation injuries ofhuman lung cells and animal model lung tissues, and can enhance oxidation resistance of the cells and cell proliferation capacity after radiation. A GCH1 overexpression adenovirus or the drug containing the GCH1 overexpression adenovirus can be used for preventing and treating human acute lung radiation injuries, and has the important significance and application value in coping with nuclear andradiation accidents and nuclear emergencies, reducing the lung radiation injury effects on tumor radiation patients and the like.
Owner:SUZHOU UNIV +1
Application of guanosine triphosphate in agriculture
PendingCN112110766APromote germinationPromote growthPlant growth regulatorsBiocideGrowth plantPlant roots
The invention relates to application of guanosine triphosphate in agriculture. The guanosine triphosphate can promote seed germination, plant root growth and plant growth.
Owner:烟台天晟农业科技有限公司
Fermentation culture medium for producing riboflavin and fermentation method
ActiveCN113817654APromote growthEasy to synthesizeBacteriaMicroorganism based processesDipotassium phosphateGlutamine
The invention discloses a fermentation culture medium for producing riboflavin and a fermentation method. The fermentation culture medium comprises the following components: 20-30 g / L of glucose, 10-20 g / L of yeast powder, 10-20 g / L of soybean cake powder, 10-20 g / L of corn steep liquor, 2-5 g / L of sodium citrate, 3-6 g / L of dipotassium phosphate, 1-3 g / L of ammonium sulfate, 0.5-1.5 g / L of sodium nitrate, 100-200 mL of glutamine mother liquor and the balance of water. The fermentation method comprises the following steps: (1) preparing the fermentation culture medium; (2) performing sterilizing; (3) performing inoculating with a bacillus subtilis seed solution; and (4) performing fermentation culture. The fermentation culture medium has the beneficial effects that the sodium citrate is added to inhibit a glycolysis pathway and increase the carbon flux of an HMP pathway; and glutamine is used as a synthesis precursor of guanosine triphosphate (GTP) and also directly promotes riboflavin generation. According to the fermentation method disclosed by the invention, the production level and the conversion rate of vitamin B2 are greatly improved, the fermentation cost is reduced, and the fermentation method disclosed by the invention is simple and easy to implement.
Owner:TONGLIAO MEIHUA BIOLOGICAL SCI TECH CO LTD
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