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Preparation and use of maize yellow powder protein hydrolysate with hypotensive function

A protein hydrolyzate and corn yellow powder technology, applied in the field of plant protein development and utilization, can solve the problems of long process, high production cost, slow hydrolysis rate, etc.

Active Publication Date: 2009-07-22
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Corn yellow powder is insoluble in water, and the traditional enzymatic hydrolysis method has disadvantages such as slow hydrolysis rate, long process, low enzyme utilization rate and high production cost.
The published invention patent "A Method for Improving the Degree of Hydrolysis and Degradation Rate of Corn Protein" (publication number: CN1710091) mentions the use of heating to increase the degree of hydrolysis and degradation rate of corn protein, but does not mention that the product can be used for adjunctive treatment of high blood pressure

Method used

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  • Preparation and use of maize yellow powder protein hydrolysate with hypotensive function
  • Preparation and use of maize yellow powder protein hydrolysate with hypotensive function
  • Preparation and use of maize yellow powder protein hydrolysate with hypotensive function

Examples

Experimental program
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Effect test

Embodiment 1

[0012] Feedstock utilizes supercritical CO 2 Oil removal, extraction pressure 30MPa, extraction temperature 35°C, separation pressure 5MPa, separation temperature 40°C, extraction time 3h. The degreased raw materials are pulverized until they all pass through a 40-mesh sieve. Weigh 50kg of the above-mentioned raw materials, add 250kg of water to prepare and carry out ultrasonic treatment, respectively put 4 concentrated ultrasonic heads with ultrasonic frequency of 20kHz on the circumference of the extraction tank, ultrasonic power 500W, ultrasonic work / intermittent time 1S / 1S, the initial temperature of the feed liquid is 20°C, and the ultrasonic time is 80min. During the process of ultrasonic treatment, the material liquid is continuously stirred to ensure uniform ultrasonic treatment. Add 0.25kg of neutral protease after ultrasonic treatment (protease activity meets the requirements of qualified products in QB / T 1805.3-93), temperature 55°C, pH 6.0, keep temperature and ...

Embodiment 2

[0023] Feedstock utilizes supercritical CO 2 Oil removal, extraction pressure 30MPa, extraction temperature 35°C, separation pressure 5MPa, separation temperature 40°C, extraction time 3h. The degreased raw materials are pulverized until they all pass through a 40-mesh sieve. Weigh 51.2kg of the above raw materials and 700kg of water to prepare and carry out ultrasonic treatment, respectively put 4 concentrated ultrasonic heads with ultrasonic frequency of 20kHz on the circumference of the extraction tank, ultrasonic power 1000W, ultrasonic work / intermittent time 3S / 3S, the initial temperature of the feed liquid is 25°C, and the ultrasonic time is 40min. During the process of ultrasonic treatment, the material liquid is continuously stirred to ensure uniform ultrasonic treatment. Add 0.15 kg of neutral protease after the ultrasonic treatment (protease activity meets the requirements of qualified products in QB / T 1805.3-93), temperature is 55°C, pH is 6.0, and the temperatur...

Embodiment 3

[0027] Feedstock utilizes supercritical CO 2Oil removal, extraction pressure 30MPa, extraction temperature 35°C, separation pressure 5MPa, separation temperature 40°C, extraction time 3h. The degreased raw materials are pulverized until they all pass through a 40-mesh sieve. Weigh 50kg of the above raw materials, 1000kg of water, and then carry out ultrasonic treatment, respectively put 4 concentrated ultrasonic heads with ultrasonic frequency of 20kHz on the circumference of the extraction tank, ultrasonic power 2000W, ultrasonic work / intermittent time 10S / 10S , the initial temperature of the feed liquid is 45°C, and the ultrasonic time is 20min. During the process of ultrasonic treatment, the material liquid is continuously stirred to ensure uniform ultrasonic treatment. Add 0.05kg of neutral protease after ultrasonic treatment (protease activity meets the requirements of qualified products in QB / T 1805.3-93), temperature 55°C, pH 6.0, keep temperature and pH constant duri...

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Abstract

The invention discloses a preparation method of a corn gluten protein hydrolyzate with blood pressure lowering function, which comprises the ordered steps of: (1) corn gluten degreasing, (2) screening, (3) water feeding for mixing, (4) ultrasonic processing, (5) zymohydrolysis, (6) centrifugation, (7) filtering and (8) drying. The preparation method of the corn gluten protein hydrolyzate has the following advantages that as a corn gluten turbid suspension is processed by shaped ultrasonic and zymohydrolysis, the preparation method of the invention raises the ACE inhibition rate of an ultrasonically processed zymohydrolysis product by 30 to 80 percent and the product yield by 30 to 70 percent when compared with normal zymohydrolysis under the same zymohydrolysis conditions. In the zymohydrolysis process, Ca(OH)2 solution is used for pH value adjusting, thus avoiding the introduction of Na ions that can raise the blood pressure of human bodies, simplifying production techniques and lowering cost. The corn gluten protein hydrolyzate that is prepared by the preparation method can be used for preparing blood pressure lowering medicines or health-care food with auxiliary blood pressure regulating function.

Description

technical field [0001] The invention relates to the field of development and utilization of plant protein, in particular to a corn flour protein hydrolyzate with the function of lowering blood pressure and a high-efficiency preparation method thereof. technical background [0002] Corn yellow powder is the leftovers of the starch industry, and its protein content is as high as 60%. Due to poor water solubility, complex composition and rough taste, it is mainly used in the production of feed or natural discharge, causing great waste and environmental pollution. Corn gluten protein contains a high proportion of hydrophobic amino acids such as Leu, Val, Ala and Pro, Glu, etc., and rarely contains basic amino acids such as Lys. This special amino acid composition makes zein contain a large number of active peptide fragments, which can be hydrolyzed to generate active peptides. [0003] At present, there have been reports on the production of active peptides from corn yellow po...

Claims

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Application Information

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IPC IPC(8): C12P21/06A61K38/01A61P9/12A23L1/29A23L33/00
CPCY02P20/54
Inventor 马海乐骆琳何荣海王振斌丁青芝
Owner JIANGSU UNIV
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