Method for extracting soy protein, oligosaccharide and isoflavone from soybean whey by two-stage two-segment film

A technology of soybean protein and soybean yellow, which is applied to the preparation methods of oligosaccharides and peptides, chemical instruments and methods, etc., to achieve the effects of reducing environmental pollution, short process and simple process

Active Publication Date: 2009-08-12
黄山佳龙绿色食品有限公司
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Problems solved by technology

[0004] In order to solve the problem of comprehensive extraction and utilization of soybean protein, oligosaccharides and isoflavones in soybean yellow pulp, the present invention provides a sim

Method used

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  • Method for extracting soy protein, oligosaccharide and isoflavone from soybean whey by two-stage two-segment film

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Experimental program
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Embodiment 1

[0027] see figure 1 The method for extracting soybean protein, oligosaccharides and isoflavones from soybean yellow pulp water by secondary two-section membrane comprises the following steps:

[0028] Step 1: Preprocessing

[0029] Collect the soybean yellow slurry water produced in the production process of soybean products to the waste water tank, and enter the disc centrifuge with a speed of more than 10000r / min and a flow rate of 2L / min through a waste water booster pump for centrifugation pretreatment, and the centrifugation temperature is 10- 60°C to remove the bean dregs, particles, suspended matter, etc., to obtain the supernatant A;

[0030] Step 2: Ultrafiltration

[0031] The supernatant A obtained after pretreatment is passed through a one-stage ultrafiltration membrane system with a molecular weight cut-off of 50,000-100,000 Daltons at a temperature of 20-50°C and a pressure of 0.5-1.5Mpa to obtain soybean protein concentrate P1 A , and get ultrafiltration memb...

Embodiment 2

[0038] Step 1: Preprocessing

[0039]Take 1000L of soybean yellow slurry water and add it to a disc centrifuge with a rotation speed of 20000r / min or more and a flow rate of 2L / min for centrifugation pretreatment. The centrifugation temperature is 10-60°C to remove the bean dregs, particles, suspended solids, etc., to obtain 974L supernatant A;

[0040] Step 2: Ultrafiltration

[0041] The supernatant A obtained after pretreatment is sequentially passed through a one-stage ultrafiltration membrane system with a molecular weight cut-off of 50,000-100,000 Daltons at 20-50°C and an operating pressure of 0.5-1.5Mpa to obtain soybean protein concentrate P1 A , and get ultrafiltration membrane permeate B 0 ; Then the ultrafiltration membrane permeate B 0 Soybean protein is separated from soy isoflavones and soybean oligosaccharides through a one-stage two-stage ultrafiltration membrane system with a molecular weight cut-off of 3000 Daltons, and soybean protein concentrate P1 is o...

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Abstract

The invention relates to a method for extracting soy protein, oligosaccharide and isoflavone by a two-stage two-section membrane, which solves the problem of comprehensive extraction and utilization of soybean protein, oligosaccharide and isoflavone in soybean yellow serofluid. The method comprises four operating steps of: preprocessing: centrifuging the soybean yellow serofluid so as to remove bean dregs, suspended matter and the like and obtain supernatant fluid A; hyperfiltration: processing the supernatant fluid A by a two-stage two-section membrane system, realizing separation of soy protein with soy isoflavones and soy oligosaccharide and obtaining soy protein finished product with the content of more than 90 percent; absorption and reverse osmosis: processing permeate liquid by a macroporous absorbent resin column and a reverse osmosis membrane system, then carrying out concentration and spray drying and obtaining the soy oligosaccharide finished product with the content of more than 80 percent; and elution: carrying out dissolution and elution to soy isoflavone absorbed by the macroporous absorbent resin with 80 percent of methanol or alcohol, then carrying out concentration and spray drying and obtaining the soy isoflavone finished product with the content of more than 50 percent. The method has simple technique and convenient operation.

Description

technical field [0001] The invention relates to extracting soybean protein, soybean oligosaccharides and soybean isoflavone functional factor products from soybean yellow slurry water by using membrane separation technology, and belongs to the technical field of soybean deep processing and waste water resource utilization. Background technique [0002] A large amount of wastewater produced in the production process of traditional tofu, dried tofu, tofu skin and soybean protein is called tofu whey wastewater, commonly known as yellow slurry water. According to statistics, every ton (t) of soybeans processed will discharge 2-5 tons (t) of yellow slurry water. Yellow pulp water is rich in widely recognized functional factors such as soybean protein, soybean oligosaccharides, soybean isoflavones and so on. After testing, it was found that the soybean isoflavones contained in the yellow pulp water accounted for 50% of the soybean isoflavones contained in the soybean raw material...

Claims

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Application Information

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IPC IPC(8): C07K14/415C07H3/06C07D311/36C07K1/34
Inventor 洪光明罗建平潘利华徐学玲查学强叶忠东
Owner 黄山佳龙绿色食品有限公司
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