Hydrolyzation of pig blood with complex enzyme method for producing blood peptide native

Abstract

The production of serum peptide hormone by the hydrolysis of pig blood with a compound enzyme method belongs to the biochemical technology field. The invention relates to the expansion of the space structure of globulin and the breaking and hydrolysis of a molecular chain, the main processes are as follows: red blood cells are extracted from fresh blood in a centrifugal way and then swelled and mixed with plasma; later pH2-3 is adjusted, and 95% of ethanol and 2.8% of H2O2 with equivalent weight are added; next, sodium hydroxide is added with the heat preserved for 2h at 80 DEG C; pH8 is adjusted, and papain, trypsin, alkaline protease and the like are added with 4h of enzymolysis at 37 DEG C and then pH3.0 is adjusted; after pepsin is added with 4h of enzymolysis at 37 DEG C, a serum peptide hormone product is obtained by enzyme destroying and drying. The technology comprises the expansion of internal gene of the globulin, the oxidation of heme, the hydrolysis of compound enzyme and the like, has over 90% of hydrolysis rate, and solves the problems of difficult hydrolysis of blood globulin and complex process in the conventional process; the serum peptide hormone product which is produced by the invention has over 60% of peptide content, simultaneously preserves the AgI immune function factors and the like in blood, enhances the organism immunity function, and is suitable for blood comprehensively processing such as slaughterhouses, etc.

Description

1. Technical field [0001] The method for producing hemopeptin by complex enzymatic hydrolysis of plasma protein proposed by the present invention and its application belong to the scientific and technological invention in the field of biotechnology. 2. Technical background [0002] Since 1993, my country's meat production has been the first in the world, and the pig production is close to half of the world's total production. From the perspective of blood production, the total bleeding volume of cattle is 4.2% of the weight of live cattle, and 3.5% of pigs. Sheep is 3.2%, and the total animal blood production in my country currently exceeds 1.2×10 6 t, but the utilization rate is very low, and the waste of resources is very serious. [0003] Countries in Europe are more advanced in the recovery and utilization of pig blood. Denmark and Sweden produce 10,000-15,000 tons of blood meal per year. The utilization rate of slaughtered blood in Sweden is 100%, and the average utiliz...

AI Technical Summary

Problems solved by technology

[0007] After the above process, add compound enzyme method to hydrolyze hemoglobin. At present, most of the hydrolyzed proteins are hydrolyzed by strong acid and strong alkali. The hydrolysis time is long, some amino acids are destroyed, and a large amount of NaCl is produced after neutralization, which is difficult to completely remove. The present invention uses compound Synergistic hydrolysis with enzymatic method solves the problem of low degree of hydrolysis by single enzyme, and also solves the problem of destruction of amino acids by strong acid and strong base hydrolysis