Hydrolyzation of pig blood with complex enzyme method for producing blood peptide native

A technology for hemolysis of blood and blood globulin, applied in the biological field, can solve the problems of low hydrolysis degree of single enzyme, long hydrolysis time, destruction of amino acids, etc.

Inactive Publication Date: 2009-08-19
SICHUAN ACAD OF FOOD & FERMENTATION INDS +1
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AI Technical Summary

Problems solved by technology

[0007] After the above process, add compound enzyme method to hydrolyze hemoglobin. At present, most of the hydrolyzed proteins are hydrolyzed by strong acid and strong alkali. The hydrolysis time is long, some amino acids are destroyed, and a large amount of NaCl is produced after neutraliza

Method used

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Embodiment Construction

[0008] (1) Take fresh pig blood and add 0.6%-1.0% trisodium citrate, stir evenly to dissolve it fully, centrifuge at 4000-5000rmp for 20 minutes, collect the precipitate; add 1:1 distilled water to the precipitate to swell the red blood cells, Broken, in order to achieve the full crushing of red blood cells, stir once every 30 minutes at high speed for 10 minutes, and mix the blood with plasma after 4 hours.

[0009] (2), adjust the pH of the above mixed solution to 2.0-3.0 with 6N HCl, add an equal volume of 95% ethanol to the blood after adjusting the pH, stir while adding, add 2.8% (blood volume) after the solution begins to become viscous count) of H 2 o 2 Stir evenly and stand still for 30 minutes. At this time, heme turns into triheme, and the solution turns from bright red to light yellow and forms a colloid. The spherical structure of hemoglobin expands to facilitate the next step of hydrolysis.

[0010] (3) Add 20% sodium hydroxide at a ratio of 3:1 to the solution,...

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Abstract

The production of serum peptide hormone by the hydrolysis of pig blood with a compound enzyme method belongs to the biochemical technology field. The invention relates to the expansion of the space structure of globulin and the breaking and hydrolysis of a molecular chain, the main processes are as follows: red blood cells are extracted from fresh blood in a centrifugal way and then swelled and mixed with plasma; later pH2-3 is adjusted, and 95% of ethanol and 2.8% of H2O2 with equivalent weight are added; next, sodium hydroxide is added with the heat preserved for 2h at 80 DEG C; pH8 is adjusted, and papain, trypsin, alkaline protease and the like are added with 4h of enzymolysis at 37 DEG C and then pH3.0 is adjusted; after pepsin is added with 4h of enzymolysis at 37 DEG C, a serum peptide hormone product is obtained by enzyme destroying and drying. The technology comprises the expansion of internal gene of the globulin, the oxidation of heme, the hydrolysis of compound enzyme and the like, has over 90% of hydrolysis rate, and solves the problems of difficult hydrolysis of blood globulin and complex process in the conventional process; the serum peptide hormone product which is produced by the invention has over 60% of peptide content, simultaneously preserves the AgI immune function factors and the like in blood, enhances the organism immunity function, and is suitable for blood comprehensively processing such as slaughterhouses, etc.

Description

1. Technical field [0001] The method for producing hemopeptin by complex enzymatic hydrolysis of plasma protein proposed by the present invention and its application belong to the scientific and technological invention in the field of biotechnology. 2. Technical background [0002] Since 1993, my country's meat production has been the first in the world, and the pig production is close to half of the world's total production. From the perspective of blood production, the total bleeding volume of cattle is 4.2% of the weight of live cattle, and 3.5% of pigs. Sheep is 3.2%, and the total animal blood production in my country currently exceeds 1.2×10 6 t, but the utilization rate is very low, and the waste of resources is very serious. [0003] Countries in Europe are more advanced in the recovery and utilization of pig blood. Denmark and Sweden produce 10,000-15,000 tons of blood meal per year. The utilization rate of slaughtered blood in Sweden is 100%, and the average utiliz...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 潘红梅孙刚游敬刚陈功伍凯戢长明
Owner SICHUAN ACAD OF FOOD & FERMENTATION INDS
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