Recombinant bacteria for characterizing genotoxicity and its construction method and application

Abstract

The invention discloses a recombinant bacterium characterizing genotoxicity, its construction method and application. The recombinant bacterium is Acinetobacter sp. ADP1 containing a reporter gene in the recA gene of the chromosome; the reporter gene and the recA gene have a common promoter. The recA gene may also contain a screening marker gene, which is located at the 3' end of the reporter gene; the screening marker gene has a promoter. After the recombinant bacteria are exposed to genotoxic substances or radiation with DNA damage ability, the reporter gene expression produces a reporter product, and the amount of the reporter product has a dose-effect relationship with the genotoxicity, which can be used to evaluate the genotoxicity of the sample and the DNA of the radiation damage intensity.

Description

technical field

[0001] The invention relates to a recombinant bacterium for characterizing genotoxicity and its construction method and application. Background technique

[0002] With the development of modern industry, environmental pollution is becoming more and more serious, and human beings discharge a large amount of toxic and harmful substances into the environment, endangering human health, so the detection of toxic substances is very important. Among them, the emphasis on genotoxic substances and the detection of genotoxicity have been the areas that have attracted much attention in the past ten years. There are usually two methods for the detection of genotoxic substances - physical and chemical methods and biological methods. Physicochemical methods are based on instrumental analysis of sample components, such as GC-MS or HPLC. The composition data of the sample can be obtained by physical and chemical means, and the toxicity of the substance in the sample can be...

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