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Process for producing abamectin by feeding glucose fermentation based on metabolizing parameters OUR

A technology of abamectin and sugar supplementation, which is applied in the field of abamectin production process based on the metabolic parameter OUR sugar supplementation fermentation, can solve the problems that affect the enthusiasm for production of abamectin, the cost of abamectin remains high, and the fermentation results Unfavorable consequences and other issues, to achieve the effect of reasonable design, lower production costs, and higher potency

Inactive Publication Date: 2009-10-21
ZHEJIANG SHENGHUA BIOK BIOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, due to the increase in the price of raw materials for production, the cost of producing abamectin remains high, which will inevitably affect the enthusiasm for producing abamectin again.
During the feeding experiment, it is necessary to determine the rate and amount of feeding according to the indicators of the fermentation process. If the feedback indicators are not obvious or there is a conflict with the bacteria according to the feeding time and rate, it will bring serious adverse consequences to the fermentation result.

Method used

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  • Process for producing abamectin by feeding glucose fermentation based on metabolizing parameters OUR
  • Process for producing abamectin by feeding glucose fermentation based on metabolizing parameters OUR
  • Process for producing abamectin by feeding glucose fermentation based on metabolizing parameters OUR

Examples

Experimental program
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Effect test

Embodiment 1

[0033] (1) Use an inoculation needle to scrape the Streptomyces avermitilis lawn on the slant medium, inoculate it into the shake flask seed medium (first-level seed medium), and cultivate it for 35 minutes at a temperature of 28°C and a shaker speed of 250r / min. Hour;

[0034] (2) The seed solution obtained in step (1) was transferred to the secondary seed medium (15L tank, about 8 liters of seed medium after sterilization), the inoculum size was 3-5ml, at a temperature of 28°C, and a speed of 200 rpm Minutes, pressure 0.05MPa, ventilation ratio 0.5vvm, cultivate for 36 hours;

[0035](3) Transplant the secondary seeds obtained in step (2) to a fermenter (50L) for fermentation and cultivation, the amount of transplantation is 8%-10% of the fermentation medium in the fermenter, i.e. a 50L tank, fermented after sterilization The culture medium is about 33L, which is about 36 liters after being transferred. The fermentation temperature is 28°C. During the fermentation process, ...

Embodiment 2

[0038] Glucose solution with a concentration of 30% is used for sugar supplementation, and the speed of sugar supplementation is: 144-168 hours of fermentation: the sugar supplementation rate is 8ml / h; 169-193 hours, the sugar supplementation rate is 13ml / h; The speed is 16ml / h, 219-243 hours, and the speed of sugar supplementation is 12ml / h. Other process conditions and steps are all the same as in Example 1, and the titer of the finally obtained abamectin is 4766u / ml, and the fermented potency without adding sugar is 4056u / ml, and the potency has increased by 17.50%.

Embodiment 3

[0040] Glucose solution with a concentration of 40% is used for sugar supplementation, and the speed of sugar supplementation is: 144-168 hours of fermentation: the sugar supplementation rate is 8ml / h; 169-193 hours, the sugar supplementation rate is 10ml / h; The speed is 12ml / h, 219-243 hours, and the speed of sugar supplementation is 8ml / h. Other process conditions and steps are all the same as in Example 1, and the titer of the finally obtained Abamectin is 4936u / ml, and the fermented titer without adding sugar is 4056u / ml, and the titer has been increased by 21.70%.

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Abstract

The invention relates to a process for producing abamectin by feeding glucose fermentation based on metabolizing parameters OUR, belonging to the technical field of bio-fermentation. The method is characterized by comprising the following processing steps of: (1) sub-culturing streptomyces avermitilis into a 250ml shake flask for primary seed cultivation; (2) sub-culturing seeds obtained from step (1) into a 15L seed tank for secondary seed cultivation; and (3) sub-culturing the seeds obtained from step (2) into a 50L fermentation tank for fermentation cultivation, measuring OUR value of exhaust gas in the fermentation process in real time and conducting glucose feeding according to the OUR value. As the process is reasonably designed, the cost is reduced, the thalli senescence in the fermentation process is effectively delayed, and the titer of abamectin is improved by about 20 percent, thereby obtaining high yield by effectively utilizing raw materials.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a process for producing avermectin by fermenting sugar supplementation based on the metabolic parameter OUR. Background technique [0002] Abamectin is a neurotoxin, and its mechanism is to act on the GABA receptors of insect neuron synapses or neuromuscular synapses, interfering with the information transmission of nerve endings in insects, that is, stimulating nerve endings to release neurotransmission inhibitor γ- Aminobutyric acid (GABA), which promotes the prolonged opening of the GABA-gated chloride ion channel, has an activation effect on the chloride ion channel, and the influx of a large amount of chloride ions causes the hyperpotential of the neural membrane, causing the neural membrane to be in a state of inhibition, thereby blocking the nerve endings The connection with the muscle paralyzes the insect, refuses to eat, and dies. Because of ...

Claims

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Application Information

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IPC IPC(8): C12P17/18C12R1/465
Inventor 储消和梁剑光储炬王永红姚云泉陈中兵黄海涛姚小员倪火荣
Owner ZHEJIANG SHENGHUA BIOK BIOLOGY
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