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Electrochemiluminescence detection device of pressurized-capillary electrochromatography

A capillary electrochromatography and electrochemistry technology is applied in the field of analytical instruments to achieve the effects of easy popularization, easy operation and high detection sensitivity

Inactive Publication Date: 2012-03-14
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, pCEC-ECL combined technology has not seen relevant patent reports at home and abroad

Method used

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  • Electrochemiluminescence detection device of pressurized-capillary electrochromatography
  • Electrochemiluminescence detection device of pressurized-capillary electrochromatography
  • Electrochemiluminescence detection device of pressurized-capillary electrochromatography

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Embodiment 1

[0029] Using a pressurized capillary electrochromatographic chemiluminescence detection device, the classic electrochemiluminescent substances triethylamine (TEA) and tripropylamine (TPA) were used as detection objects to examine the analytical performance of the device. The results are shown in figure 2 . Tests show that the device can meet the experimental requirements, with good separation effect and high sensitivity. Experimental conditions: the separation column is C 4 Cation Exchange Capillary Monolithic Columns The mobile phase is phosphate (10mmol / L, pH=7.0): acetonitrile=1:1; flow rate: 0.02mL / min; column pressure: 20psi; electrochemical potential: 1.30V; detection cell luminescent reagent: 300μL 5mmol / L ruthenium Bipyridine (containing 50mmol / LpH=7.0 phosphate buffer); PMT negative high voltage: -900V; TEA: 1.0×10 -6 mol / L, TPA: 5.0×10 -6 mol / L.

Embodiment 2

[0031] Using a pressurized capillary electrochromatographic chemiluminescence detection device, lincomycin and clindamycin were analyzed and determined. The analytical objects were quickly separated within 11 minutes, avoiding the peak tailing problem, the peak shape was sharp, and the detection limit was 2× 10 -7 mol / L. Experimental conditions: the separation column is a diamine bonded silica monolithic column; the mobile phase is phosphate buffer (10mmol / L, pH=5.0): acetonitrile=4:6; separation voltage: 12kV; electrochemical potential: 1.14V; Detection cell luminescence reagent: 300 μL 5mmol / L ruthenium bipyridine (containing 50mmol / L pH=7.0 phosphate buffer); PMT negative high voltage: -900V. Lincomycin: 1×10 -5 mol / L; Clindamycin: 1×10 -5 mol / L.

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Abstract

The invention provides an electrochemiluminescence detection device of pressurized-capillary electrochromatography, belonging to the analysis instrument. The device comprises a pressure auxiliary pump, a multichannel microflow controller, a dividing valve, a high voltage power supply, a capillary electrochromatography column, a light-emitting pool, a detection system, a computer and the like. Thepressure auxiliary pump conveys flow phase to a capillary electrochromatography separation column by the multichannel microflow controller, and the positive phase of the high voltage power supply is applied to the sampling end of the capillary electrochromatography column; a signal acquisition device used for acquiring light-emitting signals of the objects to be detected is arranged below the electrochemical light-emitting pool; the oxidation-reduction reaction is controlled by a three-electrode electrochemical system; and the output end of the signal acquisition device and the control port ofthe three-electrode electrochemical system are respectively connected with the computer. The electrochemiluminescence detection device combines the electrochemiluminescence detection technology and the pressurized-capillary electrochromatography separation technology, can realize high-efficient separation and sensitive detection with electroactive substances, and is applicable to the fields of food safety, environmental detection, biochemical analysis and the like.

Description

technical field [0001] The invention relates to an analytical instrument for separating and detecting complex samples, in particular to a combined device for pressurized capillary electrochromatography and electrochemiluminescence detection. Background technique [0002] Pressurized Capillary Electrochromatography (pCEC) is a separation mode in which pump pressure and high-voltage power supply are simultaneously applied to the capillary separation column. Through the double separation effect of pump pressure and separation voltage, pCEC combines the high efficiency of capillary electrophoresis and the high selectivity of high performance liquid chromatography. It has the characteristics of high separation efficiency, small injection volume, low reagent consumption, and easy automation. An emerging microseparation technique. At present, the main detection mode of pCEC is UV-visible spectrum detection, and UV detection has a broad spectrum, but the sensitivity is low. Due to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/62G01N21/76G01N27/403G01N30/20
Inventor 林旭聪谢增鸿吴华强胡晓燕
Owner FUZHOU UNIV